Anti-CD32B + CD32A antibody [AT10] (ab23336)
![Flow Cytometry - Anti-CD32B + CD32A antibody [AT10] (ab23336)](https://www.abcam.com/ps/products/23/ab23336/Images/ab23336-32852-anti-cd32-antibody-at10-flow-cytometry.jpg "Flow Cytometry - Anti-CD32B + CD32A antibody [AT10] (ab23336)")
Key features and details
- Mouse monoclonal [AT10] to CD32B + CD32A
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD32B + CD32A antibody [AT10] -
Description
Mouse monoclonal [AT10] to CD32B + CD32A -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Dog, Rhesus monkey
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Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in K562 cells in Flow Cytometry.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
AT10 -
Myeloma
NS1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Cellular localization
CD32B: Cell membrane. CD32A: Cell membrane. -
Database links
- Entrez Gene: 2212 Human
- Entrez Gene: 2213 Human
- Omim: 146790 Human
- Omim: 604590 Human
- SwissProt: P12318 Human
- SwissProt: P31994 Human
- Unigene: 352642 Human
- Unigene: 654395 Human
see all
Images
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Flow Cytometry - Anti-CD32B + CD32A antibody [AT10] (ab23336)Overlay histogram showing K562 cells stained with ab23336 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23336, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed K562 cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab23336 has been referenced in 12 publications.
- Bogie JFJ et al. Stearoyl-CoA desaturase-1 impairs the reparative properties of macrophages and microglia in the brain. J Exp Med 217:N/A (2020). PubMed: 32097464
- Lande R et al. CXCL4 assembles DNA into liquid crystalline complexes to amplify TLR9-mediated interferon-a production in systemic sclerosis. Nat Commun 10:1731 (2019). PubMed: 31043596
- de Stoppelaar SF et al. Toll-Like Receptor Signalling Is Not Involved in Platelet Response to Streptococcus pneumoniae In Vitro or In Vivo. PLoS One 11:e0156977 (2016). PubMed: 27253707
- Bakema JE et al. Antibody-opsonized bacteria evoke an inflammatory dendritic cell phenotype and polyfunctional Th cells by cross-talk between TLRs and FcRs. J Immunol 194:1856-66 (2015). PubMed: 25582855
- Cox N et al. Distinct Fc? receptors mediate the effect of serum amyloid p on neutrophil adhesion and fibrocyte differentiation. J Immunol 193:1701-8 (2014). PubMed: 25024390