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    cd32b--cd32a-antibody-at10-bsa-free-ab41899.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-CD32B + CD32A antibody [AT10] - BSA free (ab41899)

  • Datasheet
  • SDS
Submit a review Q&A (5)References (12)

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Flow Cytometry - Anti-CD32B + CD32A antibody [AT10] - BSA free (ab41899)

    Key features and details

    • Mouse monoclonal [AT10] to CD32B + CD32A - BSA free
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-CD32B + CD32A antibody [AT10] - BSA free
    • Description

      Mouse monoclonal [AT10] to CD32B + CD32A - BSA free
    • Host species

      Mouse
    • Tested Applications & Species

      Application Species
      Flow Cyt
      Human
      See all applications and species data
    • Immunogen

      corresponding to CD32A.

    • General notes

      This antibody clone is manufactured by Abcam.

      If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 6.97% L-Arginine
    • Carrier free

      Yes
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Monoclonal
    • Clone number

      AT10
    • Myeloma

      NS1
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Immunology
      • Adaptive Immunity
      • B Cells
      • CD
      • Immunology
      • Immunoglobulins
      • Receptors
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Neutrophil Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Mast Cell Lineage
      • Immunology
      • Innate Immunity
      • Fc Receptors

    Associated products

    • Alternative Versions

      • Anti-CD32A+CD32B antibody [AT10] (ab23336)
      • Biotin Anti-CD32B + CD32A antibody [AT10] (ab30355)
      • FITC Anti-CD32B + CD32A antibody [AT10] (ab30356)
      • PE Anti-CD32B + CD32A antibody [AT10] (ab30357)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
      • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
      • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    • Recombinant Protein

      • Recombinant human CD32A protein (ab207140)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab41899 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    Flow Cyt
    Human
    All applications
    Dog
    Application Abreviews Notes
    Flow Cyt
    Use 1µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    Notes
    Flow Cyt
    Use 1µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    Target

    • Cellular localization

      CD32B: Cell membrane. CD32A: Cell membrane.
    • Database links

      • Entrez Gene: 2212 Human
      • Entrez Gene: 2213 Human
      • Omim: 146790 Human
      • Omim: 604590 Human
      • SwissProt: P12318 Human
      • SwissProt: P31994 Human
      • Unigene: 352642 Human
      • Unigene: 654395 Human
      • Unigene: 713617 Human
      see all

    Images

    • Flow Cytometry - Anti-CD32B + CD32A antibody [AT10] - BSA free (ab41899)
      Flow Cytometry - Anti-CD32B + CD32A antibody [AT10] - BSA free (ab41899)
      Overlay histogram showing K562 cells stained with ab41899 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41899, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed K562 cells used under the same conditions.

      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (12)

    Publishing research using ab41899? Please let us know so that we can cite the reference in this datasheet.

    ab41899 has been referenced in 12 publications.

    • Zhang L  et al. C-reactive protein exacerbates epithelial-mesenchymal transition through Wnt/ß-catenin and ERK signaling in streptozocin-induced diabetic nephropathy. FASEB J 33:6551-6563 (2019). PubMed: 30794428
    • Karagiannis P  et al. IgG4 subclass antibodies impair antitumor immunity in melanoma. J Clin Invest 123:1457-74 (2013). IHC-Fr ; Human . PubMed: 23454746
    • Municio C  et al. The response of human macrophages to ß-glucans depends on the inflammatory milieu. PLoS One 8:e62016 (2013). IP ; Human . PubMed: 23637950
    • Morello A  et al. Enhancing production and cytotoxic activity of polymeric soluble FasL-based chimeric proteins by concomitant expression of soluble FasL. PLoS One 8:e73375 (2013). PubMed: 23991192
    • An JH  et al. Human SAP is a novel peptidoglycan recognition protein that induces complement-independent phagocytosis of Staphylococcus aureus. J Immunol 191:3319-27 (2013). PubMed: 23966633
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Question

    - Could you provide the IP protocol to be used with this antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 23 2012

    Answer

    Thank you for contacting us.

    The IP protcol could be found at the following link;

    https://www.abcam.com/index.html?pageconfig=resource&rid=11385

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Aug 23 2012

    Question

    Please could you advise as to whether the antibody below blocks the IgG Fc binding site when bound to the Fc receptor (CD32). I would like to use this product as a capture antibody in a sandwich ELISA using the following method. Anti-CD32 plate coating then+ CD32 then+ herceptin conjugated to detection reagent.



    Anti-CD32 antibody [AT10] - Azide free (ab41899)

    Read More

    Abcam community

    Verified customer

    Asked on May 10 2012

    Answer

    Thank you very much for your interest in ab41899 Anti-CD32 antibody [AT10] - Azide free.

    According to Bunk S et al., this antibody completly blocksFcγRII (CD32) in human PBMCs.Please see the attachment.

    To our knowledge, ab41899 has not been tested in ELISA. Therefore, I can offer a discount off a future purchase if you buy ab41899 now, test it in ELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab41899 in ELISA. I will then send a discount code. This code must be issued before purchasing ab41899 so please wait for my reply before ordering.

    2. Purchase ab41899 either by phone, fax, or online (www.abcam.com).

    3. Test it in ELISA.


    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab41899 turns out to be unsuitable for ELISA, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on May 10 2012

    Question

    Dear Sir or Madam,   I am looking for a CD32 antibody for WB which is against human CD32. Is there an antibody that detects all Isoforms at the same time or do I have to buy different antibodies for CD32A, B, C?   Additionally, I would like to know, if the antibody ab41899 is against one specific isoform or if it binds to every isoform.   Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Aug 15 2011

    Answer

    Thank you very much for your interest in our products. ab89191 (Anti-CD32 antibody [MM0270- 3L1]) from our catalogue was raised using CD32b extra cellular domain and reacts with both CD32a and CD32b. Unfortunately, it was not specifically tested if it reacts with CD32c. But since the CD32c and CD32b extra cellular domains are identical, we assume that it should also recognize CD32c. This antibody is tested and guaranteed to work in WB with human samples by our Abpromise guarantee. Please follow this link to review the datasheet of ab89191: Click here (or use the following: https://www.abcam.com/CD32-antibody-MM0270-3L1-ab89191.html). I am also happy to confirm that ab41899 (Anti-CD32 antibody [AT10]) binds to the extracellular region of CD32 and reacts with all isoforms of CD32, as described in Sandilands, G.P. et al. (2007) Immunol. 91: 204-211. Furthermore, clone AT10 has been shown to block CD32 and displace any antigen (IgG) that is bound to the receptor. This antibody is not tested and guaranteed for WB. If you would like to test this antibody in WB you might be eligible for our Abreview testing discount program. I hope this information was helpful. Please do not hesitate to contact me again with any further questions or if you would like to test ab41899 in WB.

    Read More

    Abcam Scientific Support

    Answered on Aug 15 2011

    Question

    Thank you for your quick reply. How can I be sure that the Fc portion of the whole IgG antibody molecules do not actually stimulate the Fc receptors that I intend to block?Christine.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 04 2007

    Answer

    Thank you for your enquiry. I can confirm the following details which I have obtained from the laboratories: ab41899 CD32 antibody: Unfortunately, we do not have a specific protocol for the use of this product in blocking experiments, this application is supported based upon the literature. There are a number of articles which may be helpful to you, I have listed some examples below: Larsson M, Berge J, Johansson AG, Forsum U. Human dendritic cells handling of binding, uptake and degradation of free and IgG-immune complexed dinitrophenylated human serum albumin in vitro. Immunology. 1997 Jan;90(1):138-46. PMID: 9038724 Bredius RG, Fijen CA, De Haas M, Kuijper EJ, Weening RS, Van de Winkel JG, Out TA. Role of neutrophil Fc gamma RIIa (CD32) and Fc gamma RIIIb (CD16) polymorphic forms in phagocytosis of human IgG1- and IgG3-opsonized bacteria and erythrocytes. Immunology. 1994 Dec;83(4):624-30. PMID: 7875742 Pawar P, Shin PK, Mousa SA, Ross JM, Konstantopoulos K. Fluid shear regulates the kinetics and receptor specificity of Staphylococcus aureus binding to activated platelets. J Immunol. 2004 Jul 15;173(2):1258-65. PMID: 15240718 ab46679 CD16 antibody This antibody can be used for blocking IgG binding to FcγRIII. Further details can be found in the following references: HLDA IV - PART 4: NK13.3 section - NK cell structural studies: Localization of the epitopes of hu CD16 MAbs on the molecular model of CD16, Tamm, A., et al., p. 324-326. Tamm A, Schmidt RE.: The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding. J Immunol. 1996 Aug 15;157(4):1576-81. PMID: 8759741 I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    Answered on Jul 04 2007

    Question

    Has the epitope been mapped?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 09 2007

    Answer

    As far as I am aware, there has not been a study formally identifying the minimal epitoperecognised by this antibody. However, there is some information on the epitope recognised in the original paper describing the antibody: Greenman J, Tutt AL, George AJ, Pulford KA, Stevenson GT, Glennie MJ. Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors. Mol Immunol. 1991 Nov;28(11):1243-54. Metes D, Ernst LK, Chambers WH, Sulica A, Herberman RB, Morel PA. Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene. Blood. 1998 Apr 1;91(7):2369-80. I hope that is of some help to you,

    Read More

    Abcam Scientific Support

    Answered on Feb 12 2007

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