Overview

  • Product name

    Anti-CD32B antibody - C-terminal
    See all CD32B primary antibodies
  • Description

    Rabbit polyclonal to CD32B - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 248-310 of Human CD32B (Uniprot: P31994).

  • Positive control

    • THP1 whole cell lysate; A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab151497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 34 kDa.
ICC/IF 1/100 - 1/1000.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.
  • Tissue specificity

    Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.
  • Involvement in disease

    Note=A chromosomal aberration involving FCGR2B is found in a follicular lymphoma. Translocation t(1;22)(q22;q11). The translocation leads to the hyperexpression of the receptor. This may play a role in the tumor progression.
  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain

    Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD32 antibody
    • CD32 antigen antibody
    • CD32B antibody
    • CDw32 antibody
    • Fc fragment of IgG, low affinity II, receptor for (CD32) antibody
    • Fc fragment of IgG, low affinity IIb, receptor antibody
    • Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody
    • Fc fragment of IgG, low affinity IIb, receptor for (CD32) antibody
    • Fc fragment of IgG, low affinity IIb, receptor for antibody
    • Fc gamma receptor IIB antibody
    • Fc gamma RII antibody
    • Fc gamma RIIB antibody
    • Fc-gamma RII antibody
    • Fc-gamma RII-b antibody
    • Fc-gamma-RIIb antibody
    • FCG2 antibody
    • FCG2B_HUMAN antibody
    • Fcgr2 antibody
    • FCGR2B antibody
    • FCRII antibody
    • FcRII-b antibody
    • IGFR2 antibody
    • IgG Fc receptor II beta antibody
    • IgG Fc receptor II-b antibody
    • Low affinity immunoglobulin gamma Fc region receptor II antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-b antibody
    • Ly-17 antibody
    • Lym 1 antibody
    • Lymphocyte antigen 17 antibody
    see all

Images

  • ab151497 staining CD32B in Human basophil cells by Flow Cytometry. The sample was incubated with the primary antibody (10µg/ml in MACS) for 30 minutes at 4°C. An undiluted phycoerythrin-conjugated Donkey anti-rabbit IgG was used as the secondary antibody.

    See Abreview

  • Anti-CD32B antibody - C-terminal (ab151497) at 1/1000 dilution + THP1 whole cell lysate at 30 µg

    Predicted band size: 34 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed A431 cells, labeling CD32B with ab151497 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

References

This product has been referenced in:

  • Burton OT  et al. Tissue-Specific Expression of the Low-Affinity IgG Receptor, Fc?RIIb, on Human Mast Cells. Front Immunol 9:1244 (2018). Read more (PubMed: 29928276) »
  • Kozicky LK  et al. IVIg and LPS Co-stimulation Induces IL-10 Production by Human Monocytes, Which Is Compromised by an Fc?RIIA Disease-Associated Gene Variant. Front Immunol 9:2676 (2018). Read more (PubMed: 30515163) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate, pH 6
Permeabilization
Yes - 0.1% Triton X-100
Specification
Spleen
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Oliver Burton

Verified customer

Submitted Aug 01 2016

Application
Flow Cytometry
Sample
Human Cell (Peripheral blood)
Permeabilization
Yes - BD Permeabilization buffer
Gating Strategy
CCR3+CRTH2+FceRIa+IgE+, CD19+, or CD3+
Specification
Peripheral blood
Preparation
Cell harvesting/tissue preparation method: Whole Blood
Sample buffer: FACS
Fixation
BD PhosFlow Lyse/Fix

Oliver Burton

Verified customer

Submitted Jul 29 2016

Answer

ab131051 cross reacts with CD32 a, b, and c; hence, the title of the antibody states "anti-CD32."

ab151497 is tested and guaranteed to be specific for CD32b, and is also guaranteed to work in detecting human CD32b via flow cytometry for 12 months after purchase.

Abcam also carries other products essential for Flow Cytometry such as isotype controls. To confirm that the primary antibody binding is specific and not a results of non-specific Fc receptor binding or other protein interactions. The isotype control should match the species, isotype, clonality and conjugation of the primary antibody. In this case ab37415:

https://www.abcam.com/rabbit-igg-chip-grade-ab37415.html

If testing live cells we also recommend cell viability controls such as 7-AAD or Propidium Iodide. Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized. We do carry PI and offer a 7-AAD Cell-Mediated Cytotoxicity Assay Kit.

Propidium Iodide: https://www.abcam.com/Propidium-Iodide-ab14083.html

7-AAD Kit: https://www.abcam.com/7-AADCFSE-Cytotoxicity-Assay-Kit-ab133073.html

Read More
Abreviews
Application
Flow Cytometry
Fixation
Methanol
Permeabilization
No
Sample
Human Cell (Basophils)
Specification
Basophils
Gating Strategy
Basophil
Preparation
Cell harvesting/tissue preparation method: Ficoll gradient
Sample buffer: MACS

Mr. Jack Heath

Verified customer

Submitted May 30 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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