Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP888Y] to CD32B
- Suitable for: WB, IHC-P
- Reacts with: Human
Product nameAnti-CD32B antibody [EP888Y]
See all CD32B primary antibodies
DescriptionRabbit monoclonal [EP888Y] to CD32B
SpecificityThis antibody has weak cross-reactivity with CD32A and CD32C. It detects weak signal in CD32A and CD32C over-expressed lysates at the dilution of 1/200 under the exposure time of 3 minutes, but detects strong band in CD32B over-expressed lysates at the dilution of 1:2000 under the exposure time of 15 seconds.
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human CD32B aa 250-350 (C terminal).
- Daudi cell lysate, human lymph node tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab45143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 49-55 kDa.|
|IHC-P||1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionReceptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.
Tissue specificityIs the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.
Involvement in diseaseNote=A chromosomal aberration involving FCGR2B is found in a follicular lymphoma. Translocation t(1;22)(q22;q11). The translocation leads to the hyperexpression of the receptor. This may play a role in the tumor progression.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
DomainContains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Cellular localizationCell membrane.
- Information by UniProt
- CD32 antibody
- CD32 antigen antibody
- CD32B antibody
Unpurified ab45143 staining human CD32 (1/100) in human lymph node by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
All lanes : Anti-CD32B antibody [EP888Y] (ab45143) at 1/10000 dilution (purified)
Lane 1 : Human thyroid lysate
Lane 2 : Daudi whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Observed band size: 40-44 kDa why is the actual band size different from the predicted?
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemical staining of paraffin embedded human spleen with purified ab45143 at a working dilution of 1/1000. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Lanes 1-2 & 5-6 : Anti-CD32B antibody [EP888Y] (ab45143) at 1/200 dilution (unpurified)
Lanes 3-4 : Anti-CD32B antibody [EP888Y] (ab45143) at 1/2000 dilution (unpurified)
Lane 1 : Human CD32A overexpressed lysate
Lanes 2 & 4 & 6 : Non-transfected lysate
Lane 3 : Human CD32B overexpressed lysate
Lane 5 : Human CD32C overexpressed lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 39 kDa why is the actual band size different from the predicted?
Exposure times: Lanes 1 and 2: 3 minutes, Lanes 3 and 4: 15 seconds, Lanes 5 and 6: 3 minutes.
Blocking and dilution buffer: 5% NFDM/TBST.
Anti-CD32B antibody [EP888Y] (ab45143) at 1/50000 dilution (unpurified) + Daudi cell lysate at 10 µg
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Observed band size: 49-55 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab45143 has been referenced in 17 publications.
- Gwon Y et al. Amelioration of amyloid ß-Fc?RIIb neurotoxicity and tau pathologies by targeting LYN. FASEB J 33:4300-4313 (2019). PubMed: 30540497
- Shang L et al. Vaccine-Associated Maintenance of Epithelial Integrity Correlated With Protection Against Virus Entry. J Infect Dis 218:1272-1283 (2018). PubMed: 29401315
- Arthur SE et al. Genome-wide discovery of somatic regulatory variants in diffuse large B-cell lymphoma. Nat Commun 9:4001 (2018). PubMed: 30275490
- Shang L et al. Vaccine-modified NF-kB and GR signaling in cervicovaginal epithelium correlates with protection. Mucosal Immunol N/A:N/A (2017). PubMed: 28792003
- Figueroa J et al. Exosomes from Glioma-Associated Mesenchymal Stem Cells Increase the Tumorigenicity of Glioma Stem-like Cells via Transfer of miR-1587. Cancer Res 77:5808-5819 (2017). PubMed: 28855213