Overview

  • Product name

    Anti-CD32B antibody [EP888Y]
    See all CD32B primary antibodies
  • Description

    Rabbit monoclonal [EP888Y] to CD32B
  • Host species

    Rabbit
  • Specificity

    This antibody has weak cross-reactivity with CD32A and CD32C. It detects weak signal in CD32A and CD32C over-expressed lysates at the dilution of 1/200 under the exposure time of 3 minutes, but detects strong band in CD32B over-expressed lysates at the dilution of 1:2000 under the exposure time of 15 seconds.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CD32B aa 250-350 (C terminal).

  • Positive control

    • Daudi cell lysate, human lymph node tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab45143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 49-55 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.
    • Tissue specificity

      Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.
    • Involvement in disease

      Note=A chromosomal aberration involving FCGR2B is found in a follicular lymphoma. Translocation t(1;22)(q22;q11). The translocation leads to the hyperexpression of the receptor. This may play a role in the tumor progression.
    • Sequence similarities

      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • Domain

      Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD32 antibody
      • CD32 antigen antibody
      • CD32B antibody
      • CDw32 antibody
      • Fc fragment of IgG, low affinity II, receptor for (CD32) antibody
      • Fc fragment of IgG, low affinity IIb, receptor antibody
      • Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody
      • Fc fragment of IgG, low affinity IIb, receptor for (CD32) antibody
      • Fc fragment of IgG, low affinity IIb, receptor for antibody
      • Fc gamma receptor IIB antibody
      • Fc gamma RII antibody
      • Fc gamma RIIB antibody
      • Fc-gamma RII antibody
      • Fc-gamma RII-b antibody
      • Fc-gamma-RIIb antibody
      • FCG2 antibody
      • FCG2B_HUMAN antibody
      • Fcgr2 antibody
      • FCGR2B antibody
      • FCRII antibody
      • FcRII-b antibody
      • IGFR2 antibody
      • IgG Fc receptor II beta antibody
      • IgG Fc receptor II-b antibody
      • Low affinity immunoglobulin gamma Fc region receptor II antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-b antibody
      • Ly-17 antibody
      • Lym 1 antibody
      • Lymphocyte antigen 17 antibody
      see all

    Images

    • Unpurified ab45143 staining human CD32 (1/100) in human lymph node by immunohistochemistry using paraffin embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • All lanes : Anti-CD32B antibody [EP888Y] (ab45143) at 1/10000 dilution (purified)

      Lane 1 : Human thyroid lysate
      Lane 2 : Daudi whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Observed band size: 40-44 kDa
      why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemical staining of paraffin embedded human spleen with purified ab45143 at a working dilution of 1/1000. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Lanes 1-2 & 5-6 : Anti-CD32B antibody [EP888Y] (ab45143) at 1/200 dilution (unpurified)
      Lanes 3-4 : Anti-CD32B antibody [EP888Y] (ab45143) at 1/2000 dilution (unpurified)

      Lane 1 : Human CD32A overexpressed lysate
      Lanes 2 & 4 & 6 : Non-transfected lysate
      Lane 3 : Human CD32B overexpressed lysate
      Lane 5 : Human CD32C overexpressed lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 39 kDa why is the actual band size different from the predicted?



      Exposure times: Lanes 1 and 2: 3 minutes, Lanes 3 and 4: 15 seconds, Lanes 5 and 6: 3 minutes.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-CD32B antibody [EP888Y] (ab45143) at 1/50000 dilution (unpurified) + Daudi cell lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Observed band size: 49-55 kDa why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Gwon Y  et al. Amelioration of amyloid ß-Fc?RIIb neurotoxicity and tau pathologies by targeting LYN. FASEB J 33:4300-4313 (2019). Read more (PubMed: 30540497) »
    • Figueroa J  et al. Exosomes from Glioma-Associated Mesenchymal Stem Cells Increase the Tumorigenicity of Glioma Stem-like Cells via Transfer of miR-1587. Cancer Res 77:5808-5819 (2017). Read more (PubMed: 28855213) »
    See all 15 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you for your enquiry and your interest in our products. This antibody is very specific to FcgammaR-IIB. It will not detect R-IIA or RIIC. Although this will detect all 3 isoforms for FcgammR-IIB (1, 2 and 3). I hope this will be useful for you.

    Read More

    Question
    Answer

    Thank you for your enquiry.

    I can confirm that ab33515 and ab45143antibodiesare sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

    I can confirm that for tissue culture supernatant, concentration of antibody is known to very between 1 - 3 mg/ml

    I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

    Question
    Answer

    FC gamma RIIB is not predicted to recognize FC gamma RIIA or FC gamma RIIC. Sequence homology for both isoforms is 75% in 4 residues overlapping. However, it is not guaranteed without actual testing.

    Read More

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