Recombinant
RabMAb

Recombinant Anti-CD32B antibody [EP888Y] - BSA and Azide free (ab206217)

Overview

  • Product name

    Anti-CD32B antibody [EP888Y] - BSA and Azide free
    See all CD32B primary antibodies
  • Description

    Rabbit monoclonal [EP888Y] to CD32B - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CD32B (C terminal).

  • Positive control

    • Daudi cell lysate, human lymph node tissue.
  • General notes

    ab206217 is the carrier-free version of ab45143 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab206217 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab206217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 34 kDa.

Please check the parent abID, ab45143, for more information on dilutions.

IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Target

  • Function

    Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.
  • Tissue specificity

    Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.
  • Involvement in disease

    Note=A chromosomal aberration involving FCGR2B is found in a follicular lymphoma. Translocation t(1;22)(q22;q11). The translocation leads to the hyperexpression of the receptor. This may play a role in the tumor progression.
  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain

    Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD32 antibody
    • CD32 antigen antibody
    • CD32B antibody
    • CDw32 antibody
    • Fc fragment of IgG, low affinity II, receptor for (CD32) antibody
    • Fc fragment of IgG, low affinity IIb, receptor antibody
    • Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody
    • Fc fragment of IgG, low affinity IIb, receptor for (CD32) antibody
    • Fc fragment of IgG, low affinity IIb, receptor for antibody
    • Fc gamma receptor IIB antibody
    • Fc gamma RII antibody
    • Fc gamma RIIB antibody
    • Fc-gamma RII antibody
    • Fc-gamma RII-b antibody
    • Fc-gamma-RIIb antibody
    • FCG2 antibody
    • FCG2B_HUMAN antibody
    • Fcgr2 antibody
    • FCGR2B antibody
    • FCRII antibody
    • FcRII-b antibody
    • IGFR2 antibody
    • IgG Fc receptor II beta antibody
    • IgG Fc receptor II-b antibody
    • Low affinity immunoglobulin gamma Fc region receptor II antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-b antibody
    • Ly-17 antibody
    • Lym 1 antibody
    • Lymphocyte antigen 17 antibody
    see all

Images

  • Immunohistochemical staining of paraffin embedded human spleen with purified ab45143 at a working dilution of 1/1000. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45143).

  • Unpurified ab45143 staining human CD32 (1/100) in human lymph node by immunohistochemistry using paraffin embedded tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45143).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-CD32B antibody [EP888Y] - BSA and Azide free (ab206217) + Daudi (human Burkitt's lymphoma) whole cell lysates at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Predicted band size: 34 kDa


    Exposure time: 8 seconds


    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM/TBST

References

ab206217 has not yet been referenced specifically in any publications.

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