Key features and details
- Rabbit polyclonal to CD32B (phospho Y292)
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-CD32B (phospho Y292) antibody
See all CD32B primary antibodies
DescriptionRabbit polyclonal to CD32B (phospho Y292)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- 293T cells; Rat liver tissue; Human colon carcinoma tissue.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/1000. Predicted molecular weight: 34 kDa.|
FunctionReceptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.
Tissue specificityIs the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.
Involvement in diseaseNote=A chromosomal aberration involving FCGR2B is found in a follicular lymphoma. Translocation t(1;22)(q22;q11). The translocation leads to the hyperexpression of the receptor. This may play a role in the tumor progression.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
DomainContains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Cellular localizationCell membrane.
- Information by UniProt
- CD32 antibody
- CD32 antigen antibody
- CD32B antibody
Anti-CD32B (phospho Y292) antibody (ab203877) at 1/300 dilution + 293T cell lysate
Predicted band size: 34 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling CD32B (phospho Y292) with ab203877 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat liver tissue labeling CD32B (phospho Y292) with ab203877 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
ab203877 has not yet been referenced specifically in any publications.