RecombinantRabMAb

Recombinant Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)

Submit a review Q&A (3)References (3)
Immunoprecipitation - Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)
  • Western blot - Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)
  • Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP926Y] to CD32B (phospho Y292)
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CD32B (phospho Y292) antibody [EP926Y]
    See all CD32B primary antibodies

  • Description

    Rabbit monoclonal [EP926Y] to CD32B (phospho Y292)

  • Host species

    Rabbit

  • Specificity

    Detects CD32b only when phosphorylated on Tyrosine 292.

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    corresponding to Human CD32B.

  • Positive control

    • IP: Raji whole cell lysate WB: Daudi (Human Burkitt's lymphoma lymphoblast) treated with 10mM pervanadate for 30 minutes whole cell lysate

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab68423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
Application Abreviews Notes
WB
1/50000 - 1/100000. Detects a band of approximately 40,44 kDa (predicted molecular weight: 40,44 kDa).
IP
1/20.
Notes
WB
1/50000 - 1/100000. Detects a band of approximately 40,44 kDa (predicted molecular weight: 40,44 kDa).
IP
1/20.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • Target

    • Function

      Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.

    • Tissue specificity

      Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.

    • Involvement in disease

      Note=A chromosomal aberration involving FCGR2B is found in a follicular lymphoma. Translocation t(1;22)(q22;q11). The translocation leads to the hyperexpression of the receptor. This may play a role in the tumor progression.

    • Sequence similarities

      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

    • Domain

      Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD32 antibody
        • CD32 antigen antibody
        • CD32B antibody
        • CDw32 antibody
        • Fc fragment of IgG, low affinity II, receptor for (CD32) antibody
        • Fc fragment of IgG, low affinity IIb, receptor antibody
        • Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody
        • Fc fragment of IgG, low affinity IIb, receptor for (CD32) antibody
        • Fc fragment of IgG, low affinity IIb, receptor for antibody
        • Fc gamma receptor IIB antibody
        • Fc gamma RII antibody
        • Fc gamma RIIB antibody
        • Fc-gamma RII antibody
        • Fc-gamma RII-b antibody
        • Fc-gamma-RIIb antibody
        • FCG2 antibody
        • FCG2B_HUMAN antibody
        • Fcgr2 antibody
        • FCGR2B antibody
        • FCRII antibody
        • FcRII-b antibody
        • IGFR2 antibody
        • IgG Fc receptor II beta antibody
        • IgG Fc receptor II-b antibody
        • Low affinity immunoglobulin gamma Fc region receptor II antibody
        • Low affinity immunoglobulin gamma Fc region receptor II-b antibody
        • Ly-17 antibody
        • Lym 1 antibody
        • Lymphocyte antigen 17 antibody
        see all

      Images

      • Immunoprecipitation - Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)
        Immunoprecipitation - Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)

        ab68423 at 1/20 dilution immunoprecipitating CD32B  in Raji (Human Burkitt's lymphoma) whole cell lysates.
        Lane 1 (input): Raji (Human Burkitt's lymphoma) treated with 10mM pervanadate for 30 minutes whole cell lysate 10μg
        Lane 2 (+): Raji treated with 10mM pervanadate for 30 minutes whole cell lysate
        Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68423 in Raji treated with 10mM pervanadate for 30 minutes whole cell lysate
        For western blotting, ab68423  at 1/20 dilution and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.

        Expsoure time: 1 second

        Observed MW: 40-44 KDa

      • Western blot - Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)
        Western blot - Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)
        All lanes : Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423) at 1/100000 dilution

        Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
        Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) treated with 10mM pervanadate for 30 minutes whole cell lysate

        Lysates/proteins at 15 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 40,44 kDa



        Exposure time: 5 seconds

        Observed MW: 40-44 KDa

        Blocking/diluting buffer and concentration: 5% NFDM/TBST

      • Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)
        Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)

      References (3)

      Publishing research using ab68423? Please let us know so that we can cite the reference in this datasheet.

      ab68423 has been referenced in 3 publications.

      • Rieth N  et al. Characterization of SM201, an anti-hFc?RIIB antibody not interfering with ligand binding that mediates immune complex dependent inhibition of B cells. Immunol Lett 160:145-50 (2014). PubMed: 24472604
      • Sundgren NC  et al. Coupling of fc? receptor I to fc? receptor IIb by SRC kinase mediates C-reactive protein impairment of endothelial function. Circ Res 109:1132-40 (2011). PubMed: 21940940
      • Horton HM  et al. Antibody-mediated coengagement of Fc?RIIb and B cell receptor complex suppresses humoral immunity in systemic lupus erythematosus. J Immunol 186:4223-33 (2011). PubMed: 21357255

      Customer reviews and Q&As

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      1-3 of 3 Abreviews or Q&A

      Question

      Sind die AKs specifisch für die verschiedenen Isotypen IIa oder b oder c?

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      Answer

      Vielen Dank für Ihren Anruf.

      Ich kann Ihnen nun bestätigen, dass beide Antikörper spezifisch die IIb Isoform (und alle Unterformen von dieser Isoform: IIb1, IIb2, IIb3) binden.

      Beide Immunogen sind Peptide die die folgende Sequenz einschließt: TITYpSLL.

      Die Antikörper wurden nicht experimentell mit Isoform IIa und IIc getestet. Aber diese beiden Isoformen enthalten die Immunogensequenz TITYpSLL nicht.

      Ich hoffe, diese Information ist hilfreich und wünsche Ihnen viel Erfolg mit Ihrer Forschung.

      Read More

      Question

      Does ab68423 detect CD32A, CD32B, or both?

      Read More
      Answer

      ab68423 detects CD32b and is not expected to cross-react with CD32a. However, this has not been specifically tested in the laboratory as far as I am aware.

      Read More

      Question

      Inquiry: I noticed that the Epitomics data sheet for this antibody (clone EP926Y) states that it cross reacts with mouse CD32b (phospho Y292), as determined by western blot. Abcam states that it does not cross react with mouse or rat. I'm wondering which is true, or why there is this descrepancy. This is in reference to Abcam # ab68423 or Epitomics # 2308-1. Thanks.

      Read More
      Answer

      Thank you very much for contacting us with your question.

      I apologize for the confusing discrepancy on the Abcam and Epitomics datasheets. I've gotten in touch with the Epitomics lab, and they did test the antibody once on mouse samples (brain, heart, kidney and spleen) at a 1:100,000 primary dilution but did not observe a signal. They still recommend the product for mouse reactivity based on the fact that the immunogen sequence has 100% homology with the mouse protein.

      Though Epitomics is now an Abcam company, we are still working to merge all of our policies. Abcam only lists a species as reactive if it has actually been tested and shown positive results. If the results were negative then we say the antibody does not react with that species.

      Due to the above information, I hesitate to recommend this antibody for use with mouse samples. There may be tweaks to the protocol that would produce a signal with mouse lysates (such as increasing the concentration, 1:100,000 is quite high) but we can not guarantee this antibody to work with mouse.

      I hope that this information will be useful, but please let me know if you have any further questions and I'll be happy to help. Have a nice day.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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