Recombinant Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP926Y] to CD32B (phospho Y292)
- Suitable for: WB, IP
- Reacts with: Human
Overview
-
Product name
Anti-CD32B (phospho Y292) antibody [EP926Y]
See all CD32B primary antibodies -
Description
Rabbit monoclonal [EP926Y] to CD32B (phospho Y292) -
Host species
Rabbit -
Specificity
Detects CD32b only when phosphorylated on Tyrosine 292. -
Tested Applications & Species
Application Species IP HumanWB Human -
Immunogen
corresponding to Human CD32B.
-
Positive control
- IP: Raji whole cell lysate WB: Daudi (Human Burkitt's lymphoma lymphoblast) treated with 10mM pervanadate for 30 minutes whole cell lysate
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP926Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
IP |
Human
|
WB |
Human
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/50000 - 1/100000. Detects a band of approximately 40,44 kDa (predicted molecular weight: 40,44 kDa).
|
|
IP |
1/20.
|
Notes |
---|
WB
1/50000 - 1/100000. Detects a band of approximately 40,44 kDa (predicted molecular weight: 40,44 kDa). |
IP
1/20. |
Target
-
Function
Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis. -
Tissue specificity
Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells. -
Involvement in disease
Note=A chromosomal aberration involving FCGR2B is found in a follicular lymphoma. Translocation t(1;22)(q22;q11). The translocation leads to the hyperexpression of the receptor. This may play a role in the tumor progression. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 2213 Human
- Omim: 604590 Human
- SwissProt: P31994 Human
- Unigene: 654395 Human
- Unigene: 713617 Human
-
Alternative names
- CD32 antibody
- CD32 antigen antibody
- CD32B antibody
see all
Images
-
ab68423 at 1/20 dilution immunoprecipitating CD32B in Raji (Human Burkitt's lymphoma) whole cell lysates.
Lane 1 (input): Raji (Human Burkitt's lymphoma) treated with 10mM pervanadate for 30 minutes whole cell lysate 10μg
Lane 2 (+): Raji treated with 10mM pervanadate for 30 minutes whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68423 in Raji treated with 10mM pervanadate for 30 minutes whole cell lysate
For western blotting, ab68423 at 1/20 dilution and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.Expsoure time: 1 second
Observed MW: 40-44 KDa
-
All lanes : Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423) at 1/100000 dilution
Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) treated with 10mM pervanadate for 30 minutes whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 40,44 kDaExposure time: 5 seconds
Observed MW: 40-44 KDa
Blocking/diluting buffer and concentration: 5% NFDM/TBST
Protocols
References (3)
ab68423 has been referenced in 3 publications.
- Rieth N et al. Characterization of SM201, an anti-hFc?RIIB antibody not interfering with ligand binding that mediates immune complex dependent inhibition of B cells. Immunol Lett 160:145-50 (2014). PubMed: 24472604
- Sundgren NC et al. Coupling of fc? receptor I to fc? receptor IIb by SRC kinase mediates C-reactive protein impairment of endothelial function. Circ Res 109:1132-40 (2011). PubMed: 21940940
- Horton HM et al. Antibody-mediated coengagement of Fc?RIIb and B cell receptor complex suppresses humoral immunity in systemic lupus erythematosus. J Immunol 186:4223-33 (2011). PubMed: 21357255