Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP926Y] to CD32B (phospho Y292) - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free
See all CD32B primary antibodies
DescriptionRabbit monoclonal [EP926Y] to CD32B (phospho Y292) - BSA and Azide free
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human CD32B. The exact sequence is proprietary.
- IP: Raji whole cell lysate WB: Daudi (Human Burkitt's lymphoma lymphoblast) treated with 10mM pervanadate for 30 minutes whole cell lysate
ab239861 is the carrier-free version of ab68423 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab239861 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab239861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 40,44 kDa (predicted molecular weight: 40,44 kDa).|
|IP||Use at an assay dependent concentration.|
FunctionReceptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.
Tissue specificityIs the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.
Involvement in diseaseNote=A chromosomal aberration involving FCGR2B is found in a follicular lymphoma. Translocation t(1;22)(q22;q11). The translocation leads to the hyperexpression of the receptor. This may play a role in the tumor progression.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
DomainContains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Cellular localizationCell membrane.
- Information by UniProt
- CD32 antibody
- CD32 antigen antibody
- CD32B antibody
ab68423 at 1/20 dilution immunoprecipitating CD32B in Raji (Human Burkitt's lymphoma) whole cell lysates.
Lane 1 (input): Raji (Human Burkitt's lymphoma) treated with 10mM pervanadate for 30 minutes whole cell lysate 10μg
Lane 2 (+): Raji treated with 10mM pervanadate for 30 minutes whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68423 in Raji treated with 10mM pervanadate for 30 minutes whole cell lysate
For western blotting, ab68423 at 1/20 dilution and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.
Expsoure time: 1 second
Observed MW: 40-44 KDa
All lanes : Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423) at 1/100000 dilution
Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) treated with 10mM pervanadate for 30 minutes whole cell lysate
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 40,44 kDa
Exposure time: 5 seconds
Observed MW: 40-44 KDa
Blocking/diluting buffer and concentration: 5% NFDM/TBST
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab239861 has not yet been referenced specifically in any publications.