Anti-CD33 antibody [6C5/2] (ab11032)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-CD33 antibody [6C5/2]
See all CD33 primary antibodies -
DescriptionMouse monoclonal [6C5/2] to CD33
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Host speciesMouse
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Tested applicationsSuitable for: IHC-P, Flow Cyt, ELISAmore details
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Species reactivityReacts with: Human
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Immunogen
CD33-Fc recombinant protein.
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Positive control
- IHC-P: Human tonsil FFPE tissue sections
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferConstituent: PBS
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Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone number6C5/2
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MyelomaSp2/0-Ag14
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IsotypeIgG1
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab11032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| Flow Cyt | Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| ELISA | Use at an assay dependent concentration. |
Target
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FunctionPutative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
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Tissue specificityMonocytic/myeloid lineage cells.
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Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
DomainContains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
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Post-translational
modificationsPhosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. -
Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 945 Human
- Omim: 159590 Human
- SwissProt: P20138 Human
- Unigene: 83731 Human
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Alternative names
- CD 33 antibody
- CD33 antibody
- CD33 antigen (gp67) antibody
see all
Images
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![Flow Cytometry - Anti-CD33 antibody [6C5/2] (ab11032)](https://www.abcam.com/ps/products/11/ab11032/Images/ab11032-277435-ab1103221931755.png)
Flow Cytometry - Anti-CD33 antibody [6C5/2] (ab11032)Image from Zou, Zhongcheng et al. PLoS ONE 6.9 (2011): e24559. Fig 3A.Flow Cytometry analysis of untransfected (open histogram) or Siglec-transfected CHO cells (grey histogram) were incubated with gp120 after being blocked with BSA and murine IgG, and then stained with PE-conjugated streptavidin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [6C5/2] (ab11032)](https://www.abcam.com/ps/products/11/ab11032/Images/ab11032-172917-ab11032tonsil5ugmlx20ed.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [6C5/2] (ab11032)IHC image of CD33 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11032, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Flow Cytometry - Anti-CD33 antibody [6C5/2] (ab11032)](https://www.abcam.com/ps/products/11/ab11032/Images/ab11032-277436-ab11032219317553b.png)
Flow Cytometry - Anti-CD33 antibody [6C5/2] (ab11032)Image from Zou, Zhongcheng et al. PLoS ONE 6.9 (2011): e24559. Fig 3A.Flow Cytometry analysis of untransfected (open histogram) or Siglec-transfected CHO cells (grey histogram), pretreated with neuraminidase (NA) were incubated with gp120 after being blocked with BSA and murine IgG, and then stained with PE-conjugated streptavidin.
References
This product has been referenced in:
- Zou Z et al. Siglecs Facilitate HIV-1 Infection of Macrophages through Adhesion with Viral Sialic Acids. PLoS One 6:e24559 (2011). Flow Cyt . Read more (PubMed: 21931755) »
