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    cd33-antibody-6c52-ab11032.pdf

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Immunology Cell Type Markers CD Myeloid Cells
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Anti-CD33 antibody [6C5/2] (ab11032)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (6)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [6C5/2] (ab11032)

    Key features and details

    • Mouse monoclonal [6C5/2] to CD33
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-CD33 antibody [6C5/2]
      See all CD33 primary antibodies
    • Description

      Mouse monoclonal [6C5/2] to CD33
    • Host species

      Mouse
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      CD33-Fc recombinant protein.

    • Positive control

      • IHC-P: Human tonsil FFPE tissue sections
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      6C5/2
    • Myeloma

      Sp2/0-Ag14
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Myeloid Cells
      • Stem Cells
      • Hematopoietic Progenitors
      • Hematopoietic Stem Cells
      • Human Lineage Negative

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab11032 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P (2)
    Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    Notes
    IHC-P
    Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    • Function

      Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
    • Tissue specificity

      Monocytic/myeloid lineage cells.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Domain

      Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • Post-translational
      modifications

      Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P20138 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 945 Human
      • Omim: 159590 Human
      • SwissProt: P20138 Human
      • Unigene: 83731 Human
      • Alternative names

        • CD 33 antibody
        • CD33 antibody
        • CD33 antigen (gp67) antibody
        • CD33 antigen antibody
        • CD33 molecule antibody
        • CD33_HUMAN antibody
        • FLJ00391 antibody
        • gp67 antibody
        • My9 antibody
        • Myeloid cell surface antigen CD33 antibody
        • Myeloid cell surface antigen CD33 precursor antibody
        • Myeloid differentiation antigen CD33 antibody
        • p67 antibody
        • Sialic acid binding Ig like lectin 3 antibody
        • Sialic acid binding immunoglobulin like lectin 3 antibody
        • Sialic acid-binding Ig-like lectin 3 antibody
        • SIGLEC 3 antibody
        • Siglec-3 antibody
        • SIGLEC3 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [6C5/2] (ab11032)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [6C5/2] (ab11032)

        IHC image of CD33 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11032, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (6)

      Publishing research using ab11032? Please let us know so that we can cite the reference in this datasheet.

      ab11032 has been referenced in 6 publications.

      • Souza ACP  et al. Short-Term High-Fat Diet Consumption Reduces Hypothalamic Expression of the Nicotinic Acetylcholine Receptor a7 Subunit (a7nAChR) and Affects the Anti-inflammatory Response in a Mouse Model of Sepsis. Front Immunol 10:565 (2019). PubMed: 30967878
      • Liu J  et al. Tumoral EHF predicts the efficacy of anti-PD1 therapy in pancreatic ductal adenocarcinoma. J Exp Med 216:656-673 (2019). PubMed: 30733283
      • Zhao Y  et al. Myeloid-Derived Suppressor Cells and ?dT17 Cells Contribute to the Development of Gastric MALT Lymphoma in H. felis-Infected Mice. Front Immunol 10:3104 (2019). PubMed: 32063899
      • Calcinotto A  et al. IL-23 secreted by myeloid cells drives castration-resistant prostate cancer. Nature 559:363-369 (2018). PubMed: 29950727
      • Morton JJ  et al. Dual use of hematopoietic and mesenchymal stem cells enhances engraftment and immune cell trafficking in an allogeneic humanized mouse model of head and neck cancer. Mol Carcinog 57:1651-1663 (2018). PubMed: 30129680
      • Zou Z  et al. Siglecs Facilitate HIV-1 Infection of Macrophages through Adhesion with Viral Sialic Acids. PLoS One 6:e24559 (2011). Flow Cyt . PubMed: 21931755

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-3 of 3 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD33 antibody [6C5/2]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (tonsil)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
      Permeabilization
      No
      Specification
      tonsil
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
      Fixative
      10% NBF
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 03 2019

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD33 antibody [6C5/2]

      Good
      Abreviews
      Abreviews
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Skin melanoma)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: High pH
      Permeabilization
      No
      Specification
      Skin melanoma
      Blocking step
      Hydrogen peroxide as blocking agent for 5 minute(s) · Concentration: 5% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 02 2015

      Question

      Do you know if this antibody has been tested with mouse samples? Is there any data?

      Read More

      Abcam community

      Verified customer

      Asked on Oct 26 2012

      Answer

      Thank you very much for your call yesterday and for your patience while I have been in touch with the lab regarding this antibody.

      The lab scientists confirmed that the antibody cross-reacts with mouse samples, but unfortunately we don't have any data or publications to support this. I apologize that this is not available at this time.

      Please let me know if you have further questions or comments, or if there is anything else that we can do for you. Have a nice weekend.

      Read More

      Abcam Scientific Support

      Answered on Oct 26 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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