Anti-CD33 antibody (ab203253)
Key features and details
- Rabbit polyclonal to CD33
- Suitable for: IHC-P, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD33 antibody
See all CD33 primary antibodies -
Description
Rabbit polyclonal to CD33 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD33 aa 250-350 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P20138 -
Positive control
- Human gastric carcinoma tissue. Flow Cyt (Intra): U937 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/500.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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Notes |
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IHC-P
1/100 - 1/500. When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
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Function
Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). -
Tissue specificity
Monocytic/myeloid lineage cells. -
Sequence similarities
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Post-translational
modificationsPhosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 945 Human
- Omim: 159590 Human
- SwissProt: P20138 Human
- Unigene: 83731 Human
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Alternative names
- CD 33 antibody
- CD33 antibody
- CD33 antigen (gp67) antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human gastric carcinoma tissue sections labeling CD33 with ab203253 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Intracellular Flow Cytometryanalysis of human U937 cells labeling CD33 with ab203253 (green).Control cells (blue), secondary only (light blue) and isotype control (orange).
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab203253 has been referenced in 2 publications.
- Zhang X et al. BATF2 prevents glioblastoma multiforme progression by inhibiting recruitment of myeloid-derived suppressor cells. Oncogene 40:1516-1530 (2021). PubMed: 33452462
- Chen S et al. Human telomerase reverse transcriptase recruits the ß-catenin/TCF-4 complex to transactivate chemokine (C-C motif) ligand 2 expression in colorectal cancer. Biomed Pharmacother 112:108700 (2019). PubMed: 30970512