Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% ddH20
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200.
|WB||1/100 - 1/1000. Predicted molecular weight: 40 kDa.|
|Flow Cyt||Use at an assay dependent concentration.|
FunctionPutative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
Tissue specificityMonocytic/myeloid lineage cells.
Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
DomainContains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
modificationsPhosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.
Cellular localizationCell membrane.
- Information by UniProt
- CD 33 antibody
- CD33 antibody
- CD33 antigen (gp67) antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human gastric carcinoma tissue sections labeling CD33 with ab203253 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Flow Cytometry analysis of human U937 cells labeling CD33 with ab203253 (green). Control cells (blue), secondary only (light blue) and isotype control (orange).
This product has been referenced in:
- Chen S et al. Human telomerase reverse transcriptase recruits the ß-catenin/TCF-4 complex to transactivate chemokine (C-C motif) ligand 2 expression in colorectal cancer. Biomed Pharmacother 112:108700 (2019). Read more (PubMed: 30970512) »