Product nameAnti-CD33 antibody [BLR061G]
See all CD33 primary antibodies
DescriptionRabbit monoclonal [BLR061G] to CD33
Tested applicationsSuitable for: IP, WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CD33 aa 314-364 (C terminal). The exact sequence is proprietary.
Database link: P20138
- IHC-P: Acute Myeloid leukemia tissue. WB: HEL 921.7, My-La CD4+ and THP-1 whole cell lysates. IP: HEL921.7 whole cell lysate.
This product is sold under License from Bethyl Laboratories, Inc
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 8.20
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab264414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Use 20 µl/mg lysate.
|WB||1/1000. Predicted molecular weight: 40 kDa.|
|IHC-P||1/100 - 1/500.
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE sections.
FunctionPutative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
Tissue specificityMonocytic/myeloid lineage cells.
Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
DomainContains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
modificationsPhosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.
Cellular localizationCell membrane.
- Information by UniProt
- CD 33 antibody
- CD33 antibody
- CD33 antigen (gp67) antibody
CD33 was immunoprecipitated from HEL 92.1.7 whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264414 at 20 µl/mg lysate. Western blot was performed on the immunoprecipitate using ab264414 at 1/1000 dilution.
Lane 1: ab264414 IP in HEL 92.1.7 cell lysate.
Lane 2: Control IgG IP in HEL 92.1.7 cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Lysates prepared using NETN lysis buffer.
All lanes : Anti-CD33 antibody [BLR061G] (ab264414) at 1/1000 dilution
Lane 1 : HEL 92.1.7 whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : My-La CD4+ whole cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 6 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : HRP-conjugated goat anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 40 kDa
Exposure time: 30 seconds
Formalin-fixed, paraffin-embedded Acute Myeloid Leukemia tissue stained for CD33 using ab264414 at 1/200 dilution in immunohistochemical analysis. The secondary was an HRP-conjugated goat anti-rabbit IgG antibody. Substrate: DAB.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264414 has not yet been referenced specifically in any publications.