Anti-CD33 antibody [HIM3-4] (PE/Cy5®) (ab169823)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-CD33 antibody [HIM3-4] (PE/Cy5®)
See all CD33 primary antibodies -
DescriptionMouse monoclonal [HIM3-4] to CD33 (PE/Cy5®)
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Host speciesMouse
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ConjugationPE/Cy5®. Ex: 496nm, Em: 670nm
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Tested applicationsSuitable for: Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Human normal human peripheral blood cells
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.09% Sodium azide
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Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone numberHIM3-4
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab169823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 5µl for 106 cells. ab67435 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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FunctionPutative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
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Tissue specificityMonocytic/myeloid lineage cells.
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Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
DomainContains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
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Post-translational
modificationsPhosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. -
Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 945 Human
- Omim: 159590 Human
- SwissProt: P20138 Human
- Unigene: 83731 Human
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Alternative names
- CD 33 antibody
- CD33 antibody
- CD33 antigen (gp67) antibody
see all
Images
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![Flow Cytometry - Anti-CD33 antibody [HIM3-4] (PE/Cy5®) (ab169823)](https://www.abcam.com/ps/datasheet/images/169/ab169823/CD33-Primary-antibodies-ab169823-2.jpg)
Flow Cytometry - Anti-CD33 antibody [HIM3-4] (PE/Cy5®) (ab169823)Flow cytometric analysis of normal human peripheral blood cells labeling CD33 with ab169823 at 5µl/105-106 cells (filled histogram) or PE-Cy5 Mouse IgG1 K Isotype Control (open histogram). Cells in the monocyte gate were used for analysis.
Protocols
Datasheets and documents
References
ab169823 has not yet been referenced specifically in any publications.
