Key features and details
- Mouse monoclonal [WM53] to CD33
- Suitable for: Flow Cyt, IP, WB, IHC-Fr, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CD33 antibody [WM53]
See all CD33 primary antibodies
DescriptionMouse monoclonal [WM53] to CD33
Tested applicationsSuitable for: Flow Cyt, IP, WB, IHC-Fr, ICC/IFmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Human AML cells.
- ICC: HL-60 cells Lymphatic tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from tissue culture supernatant.
- Anti-CD33 antibody [WM53] (APC) (ab118967)
- Anti-CD33 antibody [WM53] (PerCP) (ab124531)
- Anti-CD33 antibody [WM53] (APC/Cy7®) (ab206634)
- Anti-CD33 antibody [WM53] - BSA and Azide free (ab213050)
- Anti-CD33 antibody [WM53] (PE/Cy5®) (ab233448)
- Anti-CD33 antibody [WM53] (PE) (ab233577)
- Anti-CD33 antibody [WM53] (FITC) (ab234216)
- Anti-CD33 antibody [WM53] (PE/Cy7®) (ab239310)
- Anti-CD33 antibody [WM53] - BSA and Azide free (ab252263)
Our Abpromise guarantee covers the use of ab30371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 40 kDa. May also detects the smaller precursor protein at 40 kDa.|
|IHC-Fr||1/50 - 1/100.|
|ICC/IF||Use a concentration of 5 µg/ml.
Works in both 4% PFA fixed cells (10mins) and 100% MeOH fixed cells (5mins)
FunctionPutative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
Tissue specificityMonocytic/myeloid lineage cells.
Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
DomainContains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
modificationsPhosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.
Cellular localizationCell membrane.
- Information by UniProt
- CD 33 antibody
- CD33 antibody
- CD33 antigen (gp67) antibody
ab30371 staining CD33 in HL-60 cells. The cells were fixed with 100% MeOH (5min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab30371 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab30371 staining human peripheral blood monocytes by flow cytometry
ab30371 has been referenced in 1 publication.
- Ornatsky O et al. Highly multiparametric analysis by mass cytometry. J Immunol Methods 361:1-20 (2010). Flow Cyt ; Human . PubMed: 20655312