Anti-CD33 antibody [WM53] - BSA and Azide free (ab213050)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-CD33 antibody [WM53] - BSA and Azide free
See all CD33 primary antibodies -
Description
Mouse monoclonal [WM53] to CD33 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cyt, Functional Studiesmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD33. (Human AML cells).
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Positive control
- Human dendritic and mast cells; Human lymph nodes and tonsils.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
WM53 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- Anti-CD33 antibody [WM53] (Allophycocyanin) (ab118967)
- Anti-CD33 antibody [WM53] (PerCP) (ab124531)
- Anti-CD33 antibody [WM53] (Allophycocyanin/Cy7 ®) (ab206634)
- Anti-CD33 antibody [WM53] (PE/Cy5®) (ab233448)
- Anti-CD33 antibody [WM53] (Phycoerythrin) (ab233577)
- Anti-CD33 antibody [WM53] (FITC) (ab234216)
- Anti-CD33 antibody [WM53] (PE/Cy7 ®) (ab239310)
- Anti-CD33 antibody [WM53] - BSA and Azide free (ab252263)
- Anti-CD33 antibody [WM53] (ab30371)
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab213050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 2 µg/ml. | |
| Flow Cyt | Use 0.5-1µg for 106 cells. (in 0.1 ml). |
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| Functional Studies | Use at an assay dependent concentration. |
Target
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Function
Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). -
Tissue specificity
Monocytic/myeloid lineage cells. -
Sequence similarities
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Post-translational
modificationsPhosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 945 Human
- Omim: 159590 Human
- SwissProt: P20138 Human
- Unigene: 83731 Human
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Alternative names
- CD 33 antibody
- CD33 antibody
- CD33 antigen (gp67) antibody
see all
Datasheets and documents
References
This product has been referenced in:
- Yan G et al. A RIPK3-PGE2 Circuit Mediates Myeloid-Derived Suppressor Cell-Potentiated Colorectal Carcinogenesis. Cancer Res 78:5586-5599 (2018). Read more (PubMed: 30012671) »
