Key features and details
- Mouse monoclonal [WM53] to CD33 (FITC)
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Product nameAnti-CD33 antibody [WM53] (FITC)
See all CD33 primary antibodies
DescriptionMouse monoclonal [WM53] to CD33 (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Non human primates
Tissue, cells or virus corresponding to CD33. (Human AML cells).
- Flow Cytometry: Human peripheral blood.
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.0975% Sodium azide
Concentration information loading...
Purification notesab234216 is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
- Anti-CD33 antibody [WM53] (APC) (ab118967)
- Anti-CD33 antibody [WM53] (PerCP) (ab124531)
- Anti-CD33 antibody [WM53] (APC/Cy7®) (ab206634)
- Anti-CD33 antibody [WM53] - BSA and Azide free (ab213050)
- Anti-CD33 antibody [WM53] (PE/Cy5®) (ab233448)
- Anti-CD33 antibody [WM53] (PE) (ab233577)
- Anti-CD33 antibody [WM53] (PE/Cy7®) (ab239310)
- Anti-CD33 antibody [WM53] - BSA and Azide free (ab252263)
- Anti-CD33 antibody [WM53] (ab30371)
Our Abpromise guarantee covers the use of ab234216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 4µl for 106 cells.
(or 100 μL whole blood)
FunctionPutative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
Tissue specificityMonocytic/myeloid lineage cells.
Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
DomainContains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
modificationsPhosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.
Cellular localizationCell membrane.
- Information by UniProt
- CD 33 antibody
- CD33 antibody
- CD33 antigen (gp67) antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234216 has not yet been referenced specifically in any publications.