The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells.
ab154457 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
Belongs to the CD34 family.
On early hematopoietic progenitor cells.
Highly glycosylated. Phosphorylated on serine residues by PKC.
Flow cytometric analysis of normal Human peripheral blood cells with Anti-Human CD45 FITC and Mouse IgG1 kappa Isotype Control PE-Cy7 (left) or ab155358 (right). Cells in the lymphocyte gate were used for analysis.