Anti-CD34 antibody [4H11(APG)] (ab762)
Key features and details
- Mouse monoclonal [4H11(APG)] to CD34
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
-
Product name
Anti-CD34 antibody [4H11(APG)]
See all CD34 primary antibodies -
Description
Mouse monoclonal [4H11(APG)] to CD34 -
Host species
Mouse -
Specificity
This antibody reacts with Class III epitope on CD34 antigen. -
Tested applications
Suitable for: Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD34. Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia
-
Positive control
- IHC-P: Human normal placenta. WB: TF-1 Flow Cytometry: human peripheral whole blood.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
Purified from TCS. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
4H11(APG) -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use a concentration of 2 µg/ml.
|
|
IHC-P |
Use a concentration of 10 µg/ml.
|
|
WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa.
|
Notes |
---|
Flow Cyt
Use a concentration of 2 µg/ml. |
IHC-P
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa. |
Target
-
Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. -
Tissue specificity
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. -
Sequence similarities
Belongs to the CD34 family. -
Developmental stage
On early hematopoietic progenitor cells. -
Post-translational
modificationsHighly glycosylated.
Phosphorylated on serine residues by PKC. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 947 Human
- Omim: 142230 Human
- SwissProt: P28906 Human
- Unigene: 374990 Human
-
Alternative names
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
Images
-
All lanes : Anti-CD34 antibody [4H11(APG)] (ab762) at 2 µg/ml
Lane 1 : TF-1 cell line non-reducing
Lane 2 : HEK293T/17 cell line (CD34 non-expressing cell line; negative control) non reducing
Lane 3 : TF-1 cell line reducing
Lane 4 : HEK293T/17 cell line (CD34 non-expressing cell line; negative control) Reducing
Secondary
All lanes : IRDye800-conjugated anti-mouse IgG1A specific band was detected for CD34 protein at approximately 110 kDa.
-
Flow cytometric surface staining pattern showing CD34 positive stem cells in red stained with 2µg/ml ab762 in human peripheral whole blood.
-
IHC image of CD34 staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab762, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab762 has been referenced in 6 publications.
- Shi F et al. Relationship between the expression of ARHGAP25 and RhoA in non-small cell lung cancer and vasculogenic mimicry. BMC Pulm Med 22:377 (2022). PubMed: 36207695
- Yang R et al. Low PRRX1 expression and high ZEB1 expression are significantly correlated with epithelial-mesenchymal transition and tumor angiogenesis in non-small cell lung cancer. Medicine (Baltimore) 100:e24472 (2021). PubMed: 33530259
- Cheng L et al. FOXM 1 induces Vasculogenic mimicry in esophageal cancer through ß-catenin /Tcf4 signaling. Diagn Pathol 15:14 (2020). PubMed: 32035486
- Nies C et al. A Microcavity Array-Based 4D Cell Culture Platform. Bioengineering (Basel) 6:N/A (2019). PubMed: 31159244
- Cheng L et al. Aberrant expression of MYH9 and E-cadherin in esophageal squamous cell carcinoma and their relationship to vasculogenic mimicry. Int J Clin Exp Pathol 12:2205-2214 (2019). PubMed: 31934043
- Mattheij NJ et al. Dual mechanism of integrin aIIbß3 closure in procoagulant platelets. J Biol Chem 288:13325-36 (2013). PubMed: 23519467