Overview

  • Product name

    Anti-CD34 antibody [4H11[APG]] (FITC)
    See all CD34 primary antibodies
  • Description

    Mouse monoclonal [4H11[APG]] to CD34 (FITC)
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.

  • Epitope

    Class III epitope on CD34 antigen.
  • Positive control

    • Human blood cells.

Applications

Our Abpromise guarantee covers the use of ab18227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 20 µl reagent / 100 µl sample.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
    • Tissue specificity

      Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
    • Sequence similarities

      Belongs to the CD34 family.
    • Developmental stage

      On early hematopoietic progenitor cells.
    • Post-translational
      modifications

      Highly glycosylated.
      Phosphorylated on serine residues by PKC.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD34 antibody
      • CD34 antigen antibody
      • CD34 molecule antibody
      • CD34_HUMAN antibody
      • Cluster designation 34 antibody
      • cluster of differentiation 34 antibody
      • Hematopoietic progenitor cell antigen CD34 antibody
      • HPCA1 antibody
      • Mucosialin antibody
      • OTTHUMP00000034733 antibody
      • OTTHUMP00000034734 antibody
      see all

    Images

    • Surface staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 (4H11[APG]) FITC (ab18227). Total viable cells were used for analysis.

      ab18227 was used at a recommended dilution of 2 μg/ml.

    References

    This product has been referenced in:

    • Huang N  et al. Neuroprotective effect of tanshinone IIA-incubated mesenchymal stem cells on Aß25-35-induced neuroinflammation. Behav Brain Res 365:48-55 (2019). Read more (PubMed: 30831140) »
    • Wu YT  et al. A novel and feasible way to cultivate and purify endothelial progenitor cells from bone marrow of children with congenital heart diseases. Chin Med J (Engl) 125:1903-7 (2012). Flow Cyt ; Human . Read more (PubMed: 22884051) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for contacting us. The concentration of ab18227, anti-CD34, has been determined and is 50 ug/ml for the lot that you have. So, 20 ul contains 1 ug.

    Abcam also carries other products essential for Flow Cytometry such as isotype controls. If testing live cells we also recommend cell viability controls such as 7-AAD or Propidium Iodide. Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized. We do carry PI and offer a 7-AAD Cell-Mediated Cytotoxicity Assay Kit.

    Propidium Iodide: https://www.abcam.com/Propidium-Iodide-ab14083.html

    7-AAD Kit: https://www.abcam.com/7-AADCFSE-Cytotoxicity-Assay-Kit-ab133073.html

    I hope this information has been of help to you. If you have any further questions, please do not hesitate to ask.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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