• Product name
    Anti-CD34 antibody [4H11[APG]] (FITC)
    See all CD34 primary antibodies
  • Description
    Mouse monoclonal [4H11[APG]] to CD34 (FITC)
  • Host species
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    The antibody 4H11[APG] completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.

  • Epitope
    Class III epitope on CD34 antigen.
  • Positive control
    • Human blood cells.


Our Abpromise guarantee covers the use of ab18227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 20 µl reagent / 100 µl sample.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
    • Tissue specificity
      Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
    • Sequence similarities
      Belongs to the CD34 family.
    • Developmental stage
      On early hematopoietic progenitor cells.
    • Post-translational
      Highly glycosylated.
      Phosphorylated on serine residues by PKC.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • CD34 antibody
      • CD34 antigen antibody
      • CD34 molecule antibody
      • CD34_HUMAN antibody
      • Cluster designation 34 antibody
      • Hematopoietic progenitor cell antigen CD34 antibody
      • HPCA1 antibody
      • Mucosialin antibody
      • OTTHUMP00000034733 antibody
      • OTTHUMP00000034734 antibody
      see all


    • Surface staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 (4H11[APG]) FITC (ab18227). Total viable cells were used for analysis.

      ab18227 was used at a recommended dilution of 2 μg/ml.


    This product has been referenced in:
    • Wu YT  et al. A novel and feasible way to cultivate and purify endothelial progenitor cells from bone marrow of children with congenital heart diseases. Chin Med J (Engl) 125:1903-7 (2012). Flow Cyt ; Human . Read more (PubMed: 22884051) »
    • Sampat SR  et al. Growth factor priming of synovium-derived stem cells for cartilage tissue engineering. Tissue Eng Part A 17:2259-65 (2011). Flow Cyt ; Cow . Read more (PubMed: 21542714) »
    See all 3 Publications for this product

    Customer reviews and Q&As


    Thank you for contacting us. The concentration of ab18227, anti-CD34, has been determined and is 50 ug/ml for the lot that you have. So, 20 ul contains 1 ug.

    Abcam also carries other products essential for Flow Cytometry such as isotype controls. If testing live cells we also recommend cell viability controls such as 7-AAD or Propidium Iodide. Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized. We do carry PI and offer a 7-AAD Cell-Mediated Cytotoxicity Assay Kit.

    Propidium Iodide: https://www.abcam.com/Propidium-Iodide-ab14083.html

    7-AAD Kit: https://www.abcam.com/7-AADCFSE-Cytotoxicity-Assay-Kit-ab133073.html

    I hope this information has been of help to you. If you have any further questions, please do not hesitate to ask.

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