Overview

  • Product name

  • Description

    Rabbit polyclonal to CD34
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Pig, Chimpanzee, Macaque monkey, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Human CD34 aa 300 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab140811)

  • Positive control

    • This antibody gave a positive signal in Human Lung and Human Fetal Lung tissue lysates, as well as TF-1 whole cell lysate. IHC-P: Human lung FFPE tissue sections

Properties

Applications

Our Abpromise guarantee covers the use of ab150060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90, 105 kDa (predicted molecular weight: 41 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • Tissue specificity

    Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • Sequence similarities

    Belongs to the CD34 family.
  • Developmental stage

    On early hematopoietic progenitor cells.
  • Post-translational
    modifications

    Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD34 antibody
    • CD34 antigen antibody
    • CD34 molecule antibody
    • CD34_HUMAN antibody
    • Cluster designation 34 antibody
    • Hematopoietic progenitor cell antigen CD34 antibody
    • HPCA1 antibody
    • Mucosialin antibody
    • OTTHUMP00000034733 antibody
    • OTTHUMP00000034734 antibody
    see all

Images

  • Anti-CD34 antibody (ab150060) at 1 µg/ml + TF-1 (Human erythroleukaemic cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 105 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 minutes
  • All lanes : Anti-CD34 antibody (ab150060) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?
    Additional bands at: 41 kDa, 43 kDa, 55 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes
  • IHC image of CD34 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab150060, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Veron AD  et al. Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera. BMC Vet Res 14:17 (2018). Read more (PubMed: 29343270) »
  • Nakamichi M  et al. Basic Fibroblast Growth Factor Induces Angiogenic Properties of Fibrocytes to Stimulate Vascular Formation during Wound Healing. Am J Pathol 186:3203-3216 (2016). Read more (PubMed: 27773739) »
See all 2 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Flow Cytometry
Sample
Dog Cell (Olfactory stem cells)
Permeabilization
Yes - Cold methanol 90%
Gating Strategy
Living cells
Specification
Olfactory stem cells
Preparation
Cell harvesting/tissue preparation method: Cultured stem cells
Sample buffer: 10% FBS in PBS
Fixation
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 12 2017

Application
Flow Cytometry
Sample
Horse Cell (Olfactory stem cells)
Permeabilization
Yes - Cold methanol 90%
Gating Strategy
Living cells
Specification
Olfactory stem cells
Preparation
Cell harvesting/tissue preparation method: Cultured stem cells
Sample buffer: 10% FBS in PBS
Fixation
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 12 2017

Application
Flow Cytometry
Sample
Sheep Cell (Olfactory stem cells)
Permeabilization
Yes - Cold methanol 90%
Gating Strategy
Living cells
Specification
Olfactory stem cells
Preparation
Cell harvesting/tissue preparation method: Cultured stem cells
Sample buffer: 10% FBS in PBS
Fixation
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 12 2017

Application
Flow Cytometry
Sample
Rabbit Cell (Olfactory stem cells)
Permeabilization
Yes - Cold methanol 90%
Gating Strategy
Living cells
Specification
Olfactory stem cells
Preparation
Cell harvesting/tissue preparation method: Cultured stem cells
Sample buffer: 10% FBS in PBS
Fixation
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 31 2017

Application
Flow Cytometry
Sample
Monkey Cell (Olfactory stem cells)
Permeabilization
Yes - Cold Methanol 90%
Gating Strategy
Living cells
Specification
Olfactory stem cells
Preparation
Cell harvesting/tissue preparation method: Cultured stem cells
Sample buffer: 10% FBS in PBS
Fixation
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 28 2017

Application
Flow Cytometry
Sample
Mouse lemur Cell (Olfactory stem cells)
Permeabilization
Yes - Cold methanol 90%
Gating Strategy
Living cells
Specification
Olfactory stem cells
Preparation
Cell harvesting/tissue preparation method: Cultured stem cells
Sample buffer: 10% FBS in PBS
Fixation
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 28 2017

Application
Flow Cytometry
Sample
Rat Cell (Olfactory stem cells)
Permeabilization
Yes - Methanol 90%
Gating Strategy
Living cells
Specification
Olfactory stem cells
Preparation
Cell harvesting/tissue preparation method: Cultured cells
Sample buffer: 10% FBS in PBS
Fixation
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 17 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Cytrate pH6
Permeabilization
No
Specification
Lung
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde

Antoine Veron

Verified customer

Submitted Aug 11 2016

Question
Answer

Thank you for your inquiry and your interest in our products.

We would guarantee ab112120 to work with rat cells since the dye really isn't species-specific.


I am very pleased to hear you would like to use ab150060 in rat, an untested species.

This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

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