Overview

  • Product name

  • Description

    Rabbit polyclonal to CD34
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human CD34 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: P28906

  • Positive control

    • Human lung and tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab64480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use 10 mM sodium citrate pH 6.0, boiling for 10 minutes followed by 20 minutes cooling.

Target

  • Function

    Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • Tissue specificity

    Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • Sequence similarities

    Belongs to the CD34 family.
  • Developmental stage

    On early hematopoietic progenitor cells.
  • Post-translational
    modifications

    Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD34 antibody
    • CD34 antigen antibody
    • CD34 molecule antibody
    • CD34_HUMAN antibody
    • Cluster designation 34 antibody
    • cluster of differentiation 34 antibody
    • Hematopoietic progenitor cell antigen CD34 antibody
    • HPCA1 antibody
    • Mucosialin antibody
    • OTTHUMP00000034733 antibody
    • OTTHUMP00000034734 antibody
    see all

Images

  • ab64480, at a 1/100 dilution, staining CD34 in formalin fixed, paraffin embedded human lung tissue by Immunohistochemistry.
  • ab64480 staining CD34 in Human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/50) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Choi G  et al. CD99 Is Strongly Expressed in Basal Cells of the Normal Adult Epidermis and Some Subpopulations of Appendages: Comparison with Developing Fetal Skin. J Pathol Transl Med 50:361-8 (2016). Read more (PubMed: 27498544) »
  • Tokunaga T  et al. FGF-2 Stimulates the Growth of Tenogenic Progenitor Cells to Facilitate the Generation of Tenomodulin-Positive Tenocytes in a Rat Rotator Cuff Healing Model. Am J Sports Med 43:2411-22 (2015). Read more (PubMed: 26311443) »
See all 5 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate Ph 6
Sample
Rat Tissue sections (Lung, Kidneys)
Specification
Lung, Kidneys
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 15 2013

Answer

That might be a better choice than ab8158, insofar as you avoid the issue of reactivity of the anti-rat IgG secondary with your sample. For instance, the anti-rabbit IgG secondary ab96889, conjugated to Dylight 488 (similar to Alexa 488) will recognize ab64480 but not IgG rat IgG in your sample. However, it has not been tested for ICC/IF (cytochemistry), only IHC (histochemistry) on paraffin-embedded tissue, so we do not guarantee it for that application. They are similar, but not identical. Further, the immunogen is from a cytoplasmic domain, so the cells will need to be fixed and permeabilized to allow the antibody access to the cytoplasm. Have you considered Thy-1 as a marker? This paper suggest it may be useful: Haematologica. 1998 Jul;83(7):587-92. http://www.ncbi.nlm.nih.gov/pubmed/9718862?dopt=AbstractPlus We have several antibodies to this target, inculding clone MRC OX-7 which we have unconjgate (ab225) and conjugated to FITC (ab226).

Read More

Answer

Thank you for contacting us. Although we do not currently have any antibodies specifically directed against Rat CD34, we have an antibody that would be predicted to react with this protein. Ab64480 is a rabbit polyclonal antibody against human CD34. The region of the immunogen however shares 100% homology with that of Rat CD34 (SwissProt number B1PLB1). This antibody has also been characterise with IHC-P and therefore may be suitable for your customer. If they were to purchase this antibody for testing in rat samples they would be eligible for a testing discount, more details below: www.abcam.com/collaborationdiscount If your customer would be interested in this offer, please get in contact before purchase as the code needs to be issued prior to purchase. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Specification
Kidney
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric buffer, pH 6
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Sep 13 2010

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