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RecombinantRabMAb

Recombinant Anti-CD34 antibody [EP373Y] (ab81289)

  • Datasheet
  • SDS
Reviews (47)Q&A (10)References (285)

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Flow Cytometry - Anti-CD34 antibody [EP373Y] (ab81289)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EP373Y] (ab81289)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)
  • Immunohistochemistry (Frozen sections) - Anti-CD34 antibody [EP373Y] (ab81289)
  • Immunohistochemistry (Frozen sections) - Anti-CD34 antibody [EP373Y] (ab81289)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)
  • Western blot - Anti-CD34 antibody [EP373Y] (ab81289)
  • Immunoprecipitation - Anti-CD34 antibody [EP373Y] (ab81289)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EP373Y] (ab81289)
  • OI-RD Scanning - Anti-CD34 antibody [EP373Y] (ab81289)
  • Anti-CD34 antibody [EP373Y] (ab81289)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP373Y] to CD34
  • Suitable for: WB, IHC-P, ICC/IF, IP, IHC-Fr, Flow Cyt
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-CD34 antibody [EP373Y]
    See all CD34 primary antibodies
  • Description

    Rabbit monoclonal [EP373Y] to CD34
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-Fr
    Rat
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human CD34 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P28906

  • Positive control

    • Flow Cyt: TF-1 cells. WB: TF-1 cell lysate. IHC-P: Human kidney tissue; Mouse normal brain, prostate and kidney tissues; Rat kidney tissue. ICC/IF: HUVEC cells; Human embryonic stem cell-derived endothelial cells. IHC-Fr: Mouse and rat lung tissue. Rat kidney. IP: TF-1 cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 1.15 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP373Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Immunology
    • Cell Type Markers
    • CD
    • Myeloid Cells
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Stem Cells
    • Mesenchymal Stem Cells
    • Negative Markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Stem cells
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • HSC markers
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Developmental Biology
    • Lineage specification
    • Mesoderm
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-CD34 antibody [EP373Y] (ab195013)
    • HRP Anti-CD34 antibody [EP373Y] (ab195017)
    • Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
    • PE Anti-CD34 antibody [EP373Y] (ab223930)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CD34 protein (ab126924)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-CD31 antibody [P2B1] (ab24590)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab81289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-Fr
Rat
IHC-P
Mouse
Rat
Human
IP
Human
WB
Human
All applications
Sheep
Dog
Pig
African bush elephant
Application Abreviews Notes
WB (2)
1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 41 kDa).

For unpurified use at 1/10000 - 1/100000.

ab81289 is not suitable for rat and mouse species in WB application. 

IHC-P (28)
1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at  1/100 - 1/250.

ICC/IF (4)
1/100 - 1/250.

 

IP
1/30.
IHC-Fr (5)
1/50.
Flow Cyt (2)
1/50.
Notes
WB
1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 41 kDa).

For unpurified use at 1/10000 - 1/100000.

ab81289 is not suitable for rat and mouse species in WB application. 

IHC-P
1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at  1/100 - 1/250.

ICC/IF
1/100 - 1/250.

 

IP
1/30.
IHC-Fr
1/50.
Flow Cyt
1/50.

Target

  • Function

    Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • Tissue specificity

    Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • Sequence similarities

    Belongs to the CD34 family.
  • Developmental stage

    On early hematopoietic progenitor cells.
  • Post-translational
    modifications

    Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P28906 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 947 Human
    • Entrez Gene: 12490 Mouse
    • Entrez Gene: 305081 Rat
    • Omim: 142230 Human
    • SwissProt: P28906 Human
    • SwissProt: Q64314 Mouse
    • Unigene: 374990 Human
    • Unigene: 29798 Mouse
    • Unigene: 219720 Rat
    see all
  • Alternative names

    • CD34 antibody
    • CD34 antigen antibody
    • CD34 molecule antibody
    • CD34_HUMAN antibody
    • Cluster designation 34 antibody
    • cluster of differentiation 34 antibody
    • Hematopoietic progenitor cell antigen CD34 antibody
    • HPCA1 antibody
    • Mucosialin antibody
    • OTTHUMP00000034733 antibody
    • OTTHUMP00000034734 antibody
    see all

Images

  • Flow Cytometry - Anti-CD34 antibody [EP373Y] (ab81289)
    Flow Cytometry - Anti-CD34 antibody [EP373Y] (ab81289)

    Flow Cytometry analysis of TF-1 (human erythroleukemia) cells labeling CD34 with purified ab81289 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CD34 with purified ab81289 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1/500. Negative control using PBS instead of primary antibody. Counter stained with Hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EP373Y] (ab81289)
    Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EP373Y] (ab81289)

    Immunocytochemistry/Immunofluorescence analysis of HUVEC (Human umbilical vein endothelial cell line) cells labelling CD34 with purified ab81289 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counter stain.

    Control: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling CD34 with purified ab81289 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1/500. Negative control using PBS instead of primary antibody. Counter stained with Hematoxylin.

  • Immunohistochemistry (Frozen sections) - Anti-CD34 antibody [EP373Y] (ab81289)
    Immunohistochemistry (Frozen sections) - Anti-CD34 antibody [EP373Y] (ab81289)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat lung tissue labeling CD34 with ab81289 at 1/50 (11.04 µg/mL) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat lung. is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-CD34 antibody [EP373Y] (ab81289)
    Immunohistochemistry (Frozen sections) - Anti-CD34 antibody [EP373Y] (ab81289)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney tissue labeling CD34 with ab81289 at 1/50 (11.04 µg/mL) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat kidney. is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)This image is courtesy of an Abreview submitted by Mr. Rudolf Jung.
    Paraformaldehyde-fixed, paraffin-embedded mouse kidney tissue stained for CD34 using ab81289 at 1/200 dilution in immunohistochemical analysis.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)

    IHC image of CD34 staining in Mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with unpurified ab81289, 1/250 dilution, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-CD34 antibody [EP373Y] (ab81289)
    Western blot - Anti-CD34 antibody [EP373Y] (ab81289)
    purified at 1/10000 dilution + TF-1 (Human bone marrow erythroleukemia cells) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

  • Immunoprecipitation - Anti-CD34 antibody [EP373Y] (ab81289)
    Immunoprecipitation - Anti-CD34 antibody [EP373Y] (ab81289)

    CD34 was immunoprecipitated from TF-1 (Human bone marrow erythroleukemia cells) cells using purified ab81289 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab81289. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as secondary antibody at 1/1000 dilution. Blocking and dilution buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)This image is courtesy of an anonymous Abreview.
    Paraformaldehyde-fixed, paraffin-embedded mouse prostate tissue stained for CD34 using ab81289 at 1/150 dilution in immunohistochemical analysis.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EP373Y] (ab81289)
    Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EP373Y] (ab81289)Image from Földes G et al, PLoS One. 2010 May 5;5(5):e10501, Fig 3.

    Immunocytochemistry/Immunofluorescence analysis of human embryonic stem cell-derived endothelial cells labeling CD34 with unpurified ab81289 at 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.2% aTriton X-100. An Alexa Fluor® 647-conjugated secondary antibody was used at a 1/400 dilution. DAPI (blue) was used as the nuclear counter stain.

  • OI-RD Scanning - Anti-CD34 antibody [EP373Y] (ab81289)
    OI-RD Scanning - Anti-CD34 antibody [EP373Y] (ab81289)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CD34 antibody [EP373Y] (ab81289)
    Anti-CD34 antibody [EP373Y] (ab81289)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (285)

    Publishing research using ab81289? Please let us know so that we can cite the reference in this datasheet.

    ab81289 has been referenced in 285 publications.

    • Feng Y  et al. REDD1 overexpression in oral squamous cell carcinoma may predict poor prognosis and correlates with high microvessel density. Oncol Lett 19:431-441 (2020). PubMed: 31897156
    • Sasaki T  et al. Octacalcium phosphate collagen composite (OCP/Col) enhance bone regeneration in a rat model of skull defect with dural defect. Heliyon 6:e03347 (2020). PubMed: 32072051
    • Liu Q  et al. Novel Expression of EGFL7 in Osteosarcoma and Sensitivity to Cisplatin. Front Oncol 10:74 (2020). PubMed: 32117731
    • Tang Z  et al. Enhanced monoacylglycerol lipolysis by ABHD6 promotes NSCLC pathogenesis. EBioMedicine 53:102696 (2020). PubMed: 32143183
    • Sun LL  et al. LncRNA GUSBP5-AS promotes EPC migration and angiogenesis and deep vein thrombosis resolution by regulating FGF2 and MMP2/9 through the miR-223-3p/FOXO1/Akt pathway. Aging (Albany NY) 12:4506-4526 (2020). PubMed: 32156832
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 57 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD34 antibody [EP373Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Pig Cell (brain endothelia)
    Permeabilization
    Yes - 0,1% triton-x 100
    Specification
    brain endothelia
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Mrs. Annemette Boe Marnow

    Verified customer

    Submitted May 20 2020

    Western blot abreview for Anti-CD34 antibody [EP373Y]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Cell lysate - other (brain)
    Gel Running Conditions
    Non-reduced Denaturing (4-12% Genscript gel)
    Loading amount
    6 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Mrs. Annemette Boe Marnow

    Verified customer

    Submitted May 15 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CD34 antibody [EP373Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Monkey Tissue sections (brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - triton 1%
    Specification
    brain
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Mrs. Francoise Geffroy

    Verified customer

    Submitted May 04 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CD34 antibody [EP373Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - triton 1%
    Specification
    brain
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Mrs. Francoise Geffroy

    Verified customer

    Submitted May 04 2020

    Immunocytochemistry abreview for Anti-CD34 antibody [EP373Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (Human hematopoietic stem/progenitor cells)
    Permeabilization
    Yes - 0.2% Triton, 30min RT
    Specification
    Human hematopoietic stem/progenitor cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Queralt Vallmajó

    Verified customer

    Submitted Mar 18 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CD34 antibody [EP373Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 11 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CD34 antibody [EP373Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Liver
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 11 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD34 antibody [EP373Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 11 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD34 antibody [EP373Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 11 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD34 antibody [EP373Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Kidney H9 Organoids)
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney H9 Organoids
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
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    Submitted Mar 11 2020

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