Recombinant Anti-CD34 antibody [EPR2999] (ab110643)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/ab110643-123952-anti-cd34-antibody-epr2999-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2999] to CD34
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-CD34 antibody [EPR2999]
See all CD34 primary antibodies -
Description
Rabbit monoclonal [EPR2999] to CD34 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to a region within Human CD34.
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Positive control
- Human bone marrow tissue lysate TF-1 cell lysate Human kidney tissue and Human tonsil tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2999 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/50000 - 1/200000. Detects a band of approximately 120 kDa (predicted molecular weight: 41 kDa).
|
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| IHC-P | (2) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| IP |
1/50.
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| Notes |
|---|
|
WB
1/50000 - 1/200000. Detects a band of approximately 120 kDa (predicted molecular weight: 41 kDa). |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
1/50. |
Target
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Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. -
Tissue specificity
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. -
Sequence similarities
Belongs to the CD34 family. -
Developmental stage
On early hematopoietic progenitor cells. -
Post-translational
modificationsHighly glycosylated.
Phosphorylated on serine residues by PKC. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 947 Human
- Omim: 142230 Human
- SwissProt: P28906 Human
- Unigene: 374990 Human
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Alternative names
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)
ab110643 showing positive staining in Normal stomach vessels tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/ab110643-123955-anti-cd34-antibody-epr2999-western-blot.jpg)
Western blot - Anti-CD34 antibody [EPR2999] (ab110643)All lanes : Anti-CD34 antibody [EPR2999] (ab110643) at 1/50000 dilution
Lane 1 : Human bone marrow tissue lysate
Lane 2 : TF-1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/ab110643-123951-anti-cd34-antibody-epr2999-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)ab110643 showing positive staining in Normal placenta vessels tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/ab110643-123950-anti-cd34-antibody-epr2999-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)ab110643 showing positive staining in Normal lung vessels tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/ab110643-123949-anti-cd34-antibody-epr2999-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)ab110643 showing positive staining in Normal breast vessels tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/ab110643-123954-anti-cd34-antibody-epr2999-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)ab110643, at 1/100, staining CD34 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/ab110643-123953-anti-cd34-antibody-epr2999-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)ab110643, at 1/100, staining CD34 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/ab110643-12-benefits-of-recombinant-antibodies.png)
Anti-CD34 antibody [EPR2999] (ab110643)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab110643 has been referenced in 5 publications.
- Xiang R et al. Different effects of allergic rhinitis on nasal mucosa remodeling in chronic rhinosinusitis with and without nasal polyps. Eur Arch Otorhinolaryngol 276:115-130 (2019). PubMed: 30446828
- Linghu D et al. iTRAQ-based quantitative proteomic analysis and bioinformatics study of proteins in pterygia. Proteomics Clin Appl 11:N/A (2017). PubMed: 28087900
- Inoue A et al. Exercise restores muscle stem cell mobilization, regenerative capacity and muscle metabolic alterations via adiponectin/AdipoR1 activation in SAMP10 mice. J Cachexia Sarcopenia Muscle 8:370-385 (2017). PubMed: 27897419
- Wang TB et al. Sclerosing angiomatoid nodular transformation of the spleen: A case report and literature review. Oncol Lett 12:928-932 (2016). IHC ; Human . PubMed: 27446372
- Wuchter P et al. Microcavity arrays as an in vitro model system of the bone marrow niche for hematopoietic stem cells. Cell Tissue Res 364:573-84 (2016). PubMed: 26829941