Recombinant
Anti-CD34 antibody [EPR2999] (ab110643)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-CD34 antibody [EPR2999]
See all CD34 primary antibodies -
DescriptionRabbit monoclonal [EPR2999] to CD34
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, IPmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide corresponding to a region within Human CD34.
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Positive control
- Human bone marrow tissue lysate TF-1 cell lysate Human kidney tissue and Human tonsil tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR2999
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab110643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/50000 - 1/200000. Detects a band of approximately 120 kDa (predicted molecular weight: 41 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IP | 1/50. |
Target
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FunctionPossible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
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Tissue specificitySelectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
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Sequence similaritiesBelongs to the CD34 family.
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Developmental stageOn early hematopoietic progenitor cells.
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Post-translational
modificationsHighly glycosylated.
Phosphorylated on serine residues by PKC. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 947 Human
- Omim: 142230 Human
- SwissProt: P28906 Human
- Unigene: 374990 Human
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Alternative names
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
Images
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![Western blot - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/CD34-Primary-antibodies-ab110643-1.JPG)
Western blot - Anti-CD34 antibody [EPR2999] (ab110643)All lanes : Anti-CD34 antibody [EPR2999] (ab110643) at 1/50000 dilution
Lane 1 : Human bone marrow tissue lysate
Lane 2 : TF-1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 120 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/CD34-Primary-antibodies-ab110643-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)ab110643 showing positive staining in Normal stomach vessels tissue. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/CD34-Primary-antibodies-ab110643-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)ab110643 showing positive staining in Normal placenta vessels tissue. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/CD34-Primary-antibodies-ab110643-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)ab110643 showing positive staining in Normal lung vessels tissue. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/CD34-Primary-antibodies-ab110643-7.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)ab110643 showing positive staining in Normal breast vessels tissue. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/CD34-Primary-antibodies-ab110643-2.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)ab110643, at 1/100, staining CD34 in paraffin-embedded Human kidney tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/CD34-Primary-antibodies-ab110643-3.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)ab110643, at 1/100, staining CD34 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
References
This product has been referenced in:
- Wang TB et al. Sclerosing angiomatoid nodular transformation of the spleen: A case report and literature review. Oncol Lett 12:928-932 (2016). IHC ; Human . Read more (PubMed: 27446372) »
- Wuchter P et al. Microcavity arrays as an in vitro model system of the bone marrow niche for hematopoietic stem cells. Cell Tissue Res 364:573-84 (2016). Read more (PubMed: 26829941) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"