Name: Anti-CD34 antibody [EPR2999]
Brand: RabMAb
SKU: ab110643
Rating: 5 (2 reviews)

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Primary

Secondary

Protein

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Primary - Rabbit Anti-CD34 antibody [EPR2999] (ab110643)
WB, IHC-P, IP

Secondary - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC-P, WB, ELISA, IP

Protein - Recombinant Human CD34 protein (ab126924)
SDS-PAGE, HPLC

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Product name

Anti-CD34 antibody [EPR2999]
See all CD34 primary antibodies
Description

Rabbit monoclonal [EPR2999] to CD34
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, IPmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide corresponding to a region within Human CD34.
Positive control
- Human bone marrow tissue lysate TF-1 cell lysate Human kidney tissue and Human tonsil tissue
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR2999
Isotype

IgG
Research areas

Alternative Versions
- Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)
- Anti-CD34 antibody [4H11(APG)] (ab762)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human CD34 protein (ab126924)

Our Abpromise guarantee covers the use of ab110643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/50000 - 1/200000. Detects a band of approximately 120 kDa (predicted molecular weight: 41 kDa).
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP		1/50.

Function

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Tissue specificity

Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
Sequence similarities

Belongs to the CD34 family.
Developmental stage

On early hematopoietic progenitor cells.
Post-translational
modifications

Highly glycosylated.
Phosphorylated on serine residues by PKC.
Cellular localization

Membrane.
Target information above from: UniProt accession P28906 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 947 Human
- Omim: 142230 Human
- SwissProt: P28906 Human
- Unigene: 374990 Human
Alternative names
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
- CD34_HUMAN antibody
- Cluster designation 34 antibody
- cluster of differentiation 34 antibody
- Hematopoietic progenitor cell antigen CD34 antibody
- HPCA1 antibody
- Mucosialin antibody
- OTTHUMP00000034733 antibody
- OTTHUMP00000034734 antibody
see all

Western blot - Anti-CD34 antibody [EPR2999] (ab110643)

All lanes : Anti-CD34 antibody [EPR2999] (ab110643) at 1/50000 dilution

Lane 1 : Human bone marrow tissue lysate
Lane 2 : TF-1 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 41 kDa
Observed band size: 120 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)

ab110643 showing positive staining in Normal stomach vessels tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)

ab110643 showing positive staining in Normal placenta vessels tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)

ab110643 showing positive staining in Normal lung vessels tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)

ab110643 showing positive staining in Normal breast vessels tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)

ab110643, at 1/100, staining CD34 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)

ab110643, at 1/100, staining CD34 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot protocols
Immunoprecipitation protocols
Immunohistochemistry protocols

Datasheet

This product has been referenced in:

Xiang R et al. Different effects of allergic rhinitis on nasal mucosa remodeling in chronic rhinosinusitis with and without nasal polyps. Eur Arch Otorhinolaryngol 276:115-130 (2019). Read more (PubMed: 30446828) »
Linghu D et al. iTRAQ-based quantitative proteomic analysis and bioinformatics study of proteins in pterygia. Proteomics Clin Appl 11:N/A (2017). Read more (PubMed: 28087900) »

See all 5 Publications for this product

Publishing research using ab110643? Please let us know so that we can cite the reference in this datasheet.

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1-2 of 2 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Blocking step

Dako enzyme block and protein block as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 21°C

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate, pH 6

Sample

Human Tissue sections (adipose)

Specification

adipose

Permeabilization

Fixative

Formaldehyde

Dr. Jean-Martin Lapointe

Verified customer

Submitted Sep 12 2014

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0

Sample

Human Tissue sections (ovarian cancer)

Specification

ovarian cancer

Permeabilization

Fixative

Formaldehyde

Dr. C Ricciardelli

Verified customer

Submitted Aug 25 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

Target

Function

Tissue specificity

Sequence similarities

Developmental stage

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD34 antibody [EPR2999]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD34 antibody [EPR2999]

Post-translational
modifications