Overview

  • Product name

    Anti-CD34 antibody [ICO-115]
    See all CD34 primary antibodies
  • Description

    Mouse monoclonal [ICO-115] to CD34
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, IP, ICC/IF, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Tissue, cells or virus corresponding to Human CD34. Blast cells of a chronic myeloid leukemia patient.
    Database link: P28906

  • Positive control

    • KG-1 cells, tonsil, or angiosarcoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab187282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5-1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IP Use at 2-4 µg/mg of lysate.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 40 kDa.
IHC-Fr Use a concentration of 0.25 - 0.5 µg/ml.

30 minutes at room temperature

Target

  • Function

    Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • Tissue specificity

    Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • Sequence similarities

    Belongs to the CD34 family.
  • Developmental stage

    On early hematopoietic progenitor cells.
  • Post-translational
    modifications

    Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD34 antibody
    • CD34 antigen antibody
    • CD34 molecule antibody
    • CD34_HUMAN antibody
    • Cluster designation 34 antibody
    • cluster of differentiation 34 antibody
    • Hematopoietic progenitor cell antigen CD34 antibody
    • HPCA1 antibody
    • Mucosialin antibody
    • OTTHUMP00000034733 antibody
    • OTTHUMP00000034734 antibody
    see all

Images

  • Surface flow cytometric analysis of CD34 on KG-1 cells using ab187282 at 1.0 µg for 106 cells (red), and isotype control (green).

References

This product has been referenced in:

  • Qin J  et al. Antitumor effects of brucine immuno-nanoparticles on hepatocellular carcinoma in vivo. Oncol Lett 15:6137-6146 (2018). Read more (PubMed: 29731843) »
  • Wang Z  et al. Intraglandular transplantation of adipose-derived stem cells combined with platelet-rich fibrin extract for the treatment of irradiation-induced salivary gland damage. Exp Ther Med 15:795-805 (2018). Read more (PubMed: 29434684) »
See all 4 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab187282.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up