Overview

  • Product name
    Anti-CD34 antibody [MEC 14.7] (Phycoerythrin)
    See all CD34 primary antibodies
  • Description
    Rat monoclonal [MEC 14.7] to CD34 (Phycoerythrin)
  • Host species
    Rat
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    Recognises the murine CD34 cell surface glycoprotein. Murine CD34 is expressed by haematopoietic progenitor cells in the bone marrow, and also by endothelial cells.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Cells transfected with recombinant full length protein (Mouse).

Associated products

Applications

Our Abpromise guarantee covers the use of ab23830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 19662677
IHC-Fr Use at an assay dependent concentration. PubMed: 20445540
Flow Cyt Use 10µl for 106 cells.

ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • Tissue specificity
    Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • Sequence similarities
    Belongs to the CD34 family.
  • Developmental stage
    On early hematopoietic progenitor cells.
  • Post-translational
    modifications
    Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD34 antibody
    • CD34 antigen antibody
    • CD34 molecule antibody
    • CD34_HUMAN antibody
    • Cluster designation 34 antibody
    • Hematopoietic progenitor cell antigen CD34 antibody
    • HPCA1 antibody
    • Mucosialin antibody
    • OTTHUMP00000034733 antibody
    • OTTHUMP00000034734 antibody
    see all

References

This product has been referenced in:
  • Ramirez-Garcia-Luna JL  et al. Defective bone repair in diclofenac treated C57Bl6 mice with and without lipopolysaccharide induced systemic inflammation. J Cell Physiol 234:3078-3087 (2019). Read more (PubMed: 30191966) »
  • Ramirez-GarciaLuna JL  et al. Defective bone repair in mast cell-deficient Cpa3Cre/+ mice. PLoS One 12:e0174396 (2017). Read more (PubMed: 28350850) »
See all 13 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for your enquiry. Unfortunately we are not able to accurately measure the concentration of PE-conjugated antibodies but I can give you an approximate concentration for ab23830, which is 0.1mg/ml. I hope this information will be helpful. Should you require any other information, please do not hesitate to contact me.

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Application
Flow Cytometry
Sample
Mouse Cell (bone marrow)
Specification
bone marrow
Permeabilization
No

Dr. francesco del galdo

Verified customer

Submitted Oct 17 2006

Question
Answer

Thank you for your enquiry. The protein concentration of this preparation is not measured following conjugation to R-PE as the PE interferes with the estimation of ab concentration, hence the preparation is measured in "tests" as assessed by QC. However as a guide, the approximate concentration of this antibody is 0.1mg/ml. The IgG was purified on Protein G prior to conjugation. If you have any further questions then please do not hesitate to get back in touch with me.

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Question
Answer

The concentration of this antibody is 0.1mg/ml. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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