Overview

  • Product name
    Anti-CD34 antibody [MEC 14.7] (Phycoerythrin)
    See all CD34 primary antibodies
  • Description
    Rat monoclonal [MEC 14.7] to CD34 (Phycoerythrin)
  • Host species
    Rat
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    Recognises the murine CD34 cell surface glycoprotein. Murine CD34 is expressed by haematopoietic progenitor cells in the bone marrow, and also by endothelial cells.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Cells transfected with recombinant full length protein (Mouse).

Applications

Our Abpromise guarantee covers the use of ab23830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 19662677
IHC-Fr Use at an assay dependent concentration. PubMed: 20445540
Flow Cyt Use 10µl for 106 cells.

ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • Tissue specificity
    Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • Sequence similarities
    Belongs to the CD34 family.
  • Developmental stage
    On early hematopoietic progenitor cells.
  • Post-translational
    modifications
    Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD34 antibody
    • CD34 antigen antibody
    • CD34 molecule antibody
    • CD34_HUMAN antibody
    • Cluster designation 34 antibody
    • Hematopoietic progenitor cell antigen CD34 antibody
    • HPCA1 antibody
    • Mucosialin antibody
    • OTTHUMP00000034733 antibody
    • OTTHUMP00000034734 antibody
    see all

References

This product has been referenced in:
See all 10 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

Thank you for contacting us.

Your credit note ID is x.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

To receive a refund or to confirm how you would like to use the credit note please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

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Answer

I am very pleased to hear you would like to accept our offer and test ab23830 in Flow Cytometry on guinnea pig samples. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for Flow Cytometry on guinnea pig sample and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your inquiry. In this case, a rat IgG-PE isotype control would be ok. However, I suggest that it would be preferable and more appropriate to use a rat IgG2a-PE conjugated isotype control if possible Unfortunately, I am sorry to confirm we do not have any rat IgG2a-PE isotype controls available in our catalogue. I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are: www.biocompare.com http://www.biocompare.com/ProductCategories/2045/Antibodies.html I hope this information is helpful to you. Please do not hesitate to contact me if you have any further questions.

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Answer

Thank you for your enquiry. Unfortunately we are not able to accurately measure the concentration of PE-conjugated antibodies but I can give you an approximate concentration for ab23830, which is 0.1mg/ml. I hope this information will be helpful. Should you require any other information, please do not hesitate to contact me.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (bone marrow)
Specification
bone marrow
Permeabilization
No

Dr. francesco del galdo

Verified customer

Submitted Oct 17 2006

Question
Answer

Thank you for your enquiry. The protein concentration of this preparation is not measured following conjugation to R-PE as the PE interferes with the estimation of ab concentration, hence the preparation is measured in "tests" as assessed by QC. However as a guide, the approximate concentration of this antibody is 0.1mg/ml. The IgG was purified on Protein G prior to conjugation. If you have any further questions then please do not hesitate to get back in touch with me.

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Question
Answer

The concentration of this antibody is 0.1mg/ml. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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