Anti-CD34 antibody [QBEND-10] (ab8536)
![Flow Cytometry - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536-14252-anti-cd34-antibody-qbend-10-flow-cytometry.jpg "Flow Cytometry - Anti-CD34 antibody [QBEND-10] (ab8536)")
Key features and details
- Mouse monoclonal [QBEND-10] to CD34
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD34 antibody [QBEND-10]
See all CD34 primary antibodies -
Description
Mouse monoclonal [QBEND-10] to CD34 -
Host species
Mouse -
Specificity
This antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Ultrastructure studies, using an indirect immuno-gold technique on ultra thin sections of human umbilical artery, have revealed that this antigen is associated to a degree with the basoluminal surfaces of endothelium and shows a slight reaction with an intracytoplasmic vesicular compartment. Predominant staining is on the endothelial cell membrane. QBEND-10 immunoprecipitates a glycoprotein with a relative MW 110kD expressed on haematopoietic cells and on the established myeloid leukaemic cell line KG1A. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
-
Immunogen
Other Immunogen Type corresponding to CD34. Human endothelial vesicles
Database link: P28906 -
Epitope
This antibody's target epitope is distinct from those of other anti-CD34 reactive mabs as demonstrated by its lack of sensitivity to neuraminidase treatment, and fluorescence staining profiles during flow cytometry analysis of target cell populations. -
Positive control
- tonsil, bone marrow
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
QBEND-10 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/50.
Mmethanol fixed cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| IHC-P | (4) |
1/50 - 1/100. Do not perform antigen retrieval.
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
|
| Notes |
|---|
|
Flow Cyt
1/50. Mmethanol fixed cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-P
1/50 - 1/100. Do not perform antigen retrieval. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa. |
Target
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Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. -
Tissue specificity
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. -
Sequence similarities
Belongs to the CD34 family. -
Developmental stage
On early hematopoietic progenitor cells. -
Post-translational
modificationsHighly glycosylated.
Phosphorylated on serine residues by PKC. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 947 Human
- Omim: 142230 Human
- SwissProt: P28906 Human
- Unigene: 374990 Human
-
Alternative names
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
Images
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Flow Cytometry - Anti-CD34 antibody [QBEND-10] (ab8536)Overlay histogram showing Jurkat cells stained with ab8536 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8536, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in Jurkat cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
![Western blot - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536-14253-anti-cd34-antibody-qbend-10-western-blot.jpg)
Western blot - Anti-CD34 antibody [QBEND-10] (ab8536)Anti-CD34 antibody [QBEND-10] (ab8536) at 5 µg/ml + Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 100 kDa (possible glycosylated form) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536-219201-ab8536ihcp20x.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labelling CD34 with ab8536 at 1µg/ml (1:1000). Blood vessels show positive staining.
Detection system polymeric HRP. HIER with Pressure cooker pH6.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536-268998-ab8536.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)This image is courtesy of an Abreview by Carl Hobbs.ab8536 staining CD34 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in blocking buffer) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536-219202-ab8536ihcp40x.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labelling CD34 with ab8536 at 1µg/ml (1:1000). Blood vessels show positive staining.
Detection system polymeric HRP. HIER with Pressure cooker pH6.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536-273517-ab8536IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling CD34 with ab8536 at 1ug/ml.
Detection system polymeric HRP. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536-273518-ab853616832.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)This image is courtesy of an abreview submitted by Manoj Kumar Valluru.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human umbilical cord labeling CD34 with ab8536 at 1/1000 dilution. Tissue was fixed in formaldehyde and permeabilized with PBS. Heat mediated antigen retrieval was performed using a citrate pH 6 buffer. Tissue was blocked with 5% serum for 1 hour at room temperature. A biotinylated polyclonal goat anti-mouse secondary antibody was used at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)This image is courtesy of an Abreview submitted by Dr Jose Javier Martin De Llanoab8536 at 1/500 staining human umbilical cord tissue sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with horse serum before incubation with the antibody for 16 hours. A biotinylated goat anti-mouse IgG was used as the secondary.
Protocols
References (43)
ab8536 has been referenced in 43 publications.
- Liang Y et al. Exosomal microRNA-144 from bone marrow-derived mesenchymal stem cells inhibits the progression of non-small cell lung cancer by targeting CCNE1 and CCNE2. Stem Cell Res Ther 11:87 (2020). PubMed: 32102682
- Venhuizen JH et al. Differential expression of p120-catenin 1 and 3 isoforms in epithelial tissues. Sci Rep 9:90 (2019). PubMed: 30643202
- Kim HS et al. Morphological characteristics of vasculogenic mimicry and its correlation with EphA2 expression in gastric adenocarcinoma. Sci Rep 9:3414 (2019). PubMed: 30833656
- Lu M et al. Enhanced wound healing promotion by immune response-free monkey autologous iPSCs and exosomes vs. their allogeneic counterparts. EBioMedicine 42:443-457 (2019). PubMed: 30926422
- Grahn A et al. Volumetric imaging: a potential tool to stage upper tract urothelial carcinoma. World J Urol N/A:N/A (2019). PubMed: 30820651
