Anti-CD34 antibody [QBEND-10] (ab8536)
- Datasheet
- References (31)
- Protocols
Overview
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Product nameAnti-CD34 antibody [QBEND-10]
See all CD34 primary antibodies -
DescriptionMouse monoclonal [QBEND-10] to CD34
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Host speciesMouse
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SpecificityThis antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Ultrastructure studies, using an indirect immuno-gold technique on ultra thin sections of human umbilical artery, have revealed that this antigen is associated to a degree with the basoluminal surfaces of endothelium and shows a slight reaction with an intracytoplasmic vesicular compartment. Predominant staining is on the endothelial cell membrane. QBEND-10 immunoprecipitates a glycoprotein with a relative MW 110kD expressed on haematopoietic cells and on the established myeloid leukaemic cell line KG1A.
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Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IP, IHC-P, WBmore details
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Species reactivityReacts with: Human, Cynomolgus monkey, Rhesus monkey
Does not react with: Rat, Sheep, Cow, Dog -
Immunogen
Other Immunogen Type corresponding to CD34. Human endothelial vesicles
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EpitopeThis antibody's target epitope is distinct from those of other anti-CD34 reactive mabs as demonstrated by its lack of sensitivity to neuraminidase treatment, and fluorescence staining profiles during flow cytometry analysis of target cell populations.
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Positive control
- tonsil, bone marrow
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.1% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberQBEND-10
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Myelomaunknown
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IsotypeIgG1
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab8536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/50. Mmethanol fixed cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | 1/50 - 1/100. Do not perform antigen retrieval. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 40 kDa. |
Target
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FunctionPossible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
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Tissue specificitySelectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
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Sequence similaritiesBelongs to the CD34 family.
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Developmental stageOn early hematopoietic progenitor cells.
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Post-translational
modificationsHighly glycosylated.
Phosphorylated on serine residues by PKC. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 101866926 Cynomolgus monkey
- Entrez Gene: 947 Human
- Entrez Gene: 713858 Rhesus monkey
- Omim: 142230 Human
- SwissProt: P28906 Human
- Unigene: 374990 Human
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Alternative names
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
Images
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![Flow Cytometry - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/CD34-Primary-antibodies-ab8536-8.jpg)
Flow Cytometry - Anti-CD34 antibody [QBEND-10] (ab8536)Overlay histogram showing Jurkat cells stained with ab8536 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8536, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in Jurkat cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
![Western blot - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/CD34-Primary-antibodies-ab8536-6.jpg)
Western blot - Anti-CD34 antibody [QBEND-10] (ab8536)Anti-CD34 antibody [QBEND-10] (ab8536) at 5 µg/ml + Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 100 kDa (possible glycosylated form) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536-219201-ab8536ihcp20x.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labelling CD34 with ab8536 at 1µg/ml (1:1000). Blood vessels show positive staining.
Detection system polymeric HRP. HIER with Pressure cooker pH6.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536-268998-ab8536.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)This image is courtesy of an Abreview by Carl Hobbs.ab8536 staining CD34 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in blocking buffer) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536-219202-ab8536ihcp40x.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labelling CD34 with ab8536 at 1µg/ml (1:1000). Blood vessels show positive staining.
Detection system polymeric HRP. HIER with Pressure cooker pH6.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536-273517-ab8536IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling CD34 with ab8536 at 1ug/ml.
Detection system polymeric HRP. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536-273518-ab853616832.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)This image is courtesy of an abreview submitted by Manoj Kumar Valluru.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human umbilical cord labeling CD34 with ab8536 at 1/1000 dilution. Tissue was fixed in formaldehyde and permeabilized with PBS. Heat mediated antigen retrieval was performed using a citrate pH 6 buffer. Tissue was blocked with 5% serum for 1 hour at room temperature. A biotinylated polyclonal goat anti-mouse secondary antibody was used at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)This image is courtesy of an Abreview submitted by Dr Jose Javier Martin De Llanoab8536 at 1/500 staining human umbilical cord tissue sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with horse serum before incubation with the antibody for 16 hours. A biotinylated goat anti-mouse IgG was used as the secondary.
Protocols
References
This product has been referenced in:
- Barbhuiya MA et al. Identification of spleen tyrosine kinase as a potential therapeutic target for esophageal squamous cell carcinoma using reverse phase protein arrays. Oncotarget 9:18422-18434 (2018). Read more (PubMed: 29719615) »
- Rozza-de-Menezes RE et al. Prevalence and clinicopathological characteristics of lipomatous neurofibromas in neurofibromatosis 1: An investigation of 229 cutaneous neurofibromas and a systematic review of the literature. J Cutan Pathol 45:743-753 (2018). Read more (PubMed: 29959804) »
