Overview

  • Product name
    Anti-CD34 antibody [QBEND-10]
    See all CD34 primary antibodies
  • Description
    Mouse monoclonal [QBEND-10] to CD34
  • Host species
    Mouse
  • Specificity
    This antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Ultrastructure studies, using an indirect immuno-gold technique on ultra thin sections of human umbilical artery, have revealed that this antigen is associated to a degree with the basoluminal surfaces of endothelium and shows a slight reaction with an intracytoplasmic vesicular compartment. Predominant staining is on the endothelial cell membrane. QBEND-10 immunoprecipitates a glycoprotein with a relative MW 110kD expressed on haematopoietic cells and on the established myeloid leukaemic cell line KG1A.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human, Cynomolgus monkey, Rhesus monkey
    Does not react with: Rat, Sheep, Cow, Dog
  • Immunogen

    Other Immunogen Type corresponding to CD34. Human endothelial vesicles

  • Epitope
    This antibody's target epitope is distinct from those of other anti-CD34 reactive mabs as demonstrated by its lack of sensitivity to neuraminidase treatment, and fluorescence staining profiles during flow cytometry analysis of target cell populations.
  • Positive control
    • tonsil, bone marrow

Properties

Applications

Our Abpromise guarantee covers the use of ab8536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50.

Mmethanol fixed cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P 1/50 - 1/100. Do not perform antigen retrieval.
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.

Target

  • Function
    Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • Tissue specificity
    Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • Sequence similarities
    Belongs to the CD34 family.
  • Developmental stage
    On early hematopoietic progenitor cells.
  • Post-translational
    modifications
    Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD34 antibody
    • CD34 antigen antibody
    • CD34 molecule antibody
    • CD34_HUMAN antibody
    • Cluster designation 34 antibody
    • Hematopoietic progenitor cell antigen CD34 antibody
    • HPCA1 antibody
    • Mucosialin antibody
    • OTTHUMP00000034733 antibody
    • OTTHUMP00000034734 antibody
    see all

Images

  • Overlay histogram showing Jurkat cells stained with ab8536 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8536, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in Jurkat cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Anti-CD34 antibody [QBEND-10] (ab8536) at 5 µg/ml + Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa
    Additional bands at: 100 kDa (possible glycosylated form)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labelling CD34 with ab8536 at 1µg/ml (1:1000). Blood vessels show positive staining.

    Detection system polymeric HRP. HIER with Pressure cooker pH6.

  • ab8536 staining CD34 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in blocking buffer) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labelling CD34 with ab8536 at 1µg/ml (1:1000). Blood vessels show positive staining.

    Detection system polymeric HRP. HIER with Pressure cooker pH6.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling CD34 with ab8536 at 1ug/ml.
    Detection system polymeric HRP.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human umbilical cord labeling CD34 with ab8536 at 1/1000 dilution. Tissue was fixed in formaldehyde and permeabilized with PBS. Heat mediated antigen retrieval was performed using a citrate pH 6 buffer. Tissue was blocked with 5% serum for 1 hour at room temperature. A biotinylated polyclonal goat anti-mouse secondary antibody was used at 1/100 dilution.

    See Abreview

  • ab8536 at 1/500 staining human umbilical cord tissue sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with horse serum before incubation with the antibody for 16 hours. A biotinylated goat anti-mouse IgG was used as the secondary.

    See Abreview

References

This product has been referenced in:
  • Venhuizen JH  et al. Differential expression of p120-catenin 1 and 3 isoforms in epithelial tissues. Sci Rep 9:90 (2019). Read more (PubMed: 30643202) »
  • Kim HS  et al. Morphological characteristics of vasculogenic mimicry and its correlation with EphA2 expression in gastric adenocarcinoma. Sci Rep 9:3414 (2019). Read more (PubMed: 30833656) »
See all 37 Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Sep 23 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Umbilical cord)
Specification
Umbilical cord
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization
Yes - PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Mr. Manoj Kumar Valluru

Verified customer

Submitted Dec 04 2009

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain tumor)
Specification
brain tumor
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Perkin-Elmer TNB Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 30 2009

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Umbilical cord)
Specification
Umbilical cord
Fixative
Paraformaldehyde
Antigen retrieval step
None
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2%

Dr. Jose Javier Martin De Llano

Verified customer

Submitted May 15 2007

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