Overview

  • Product name

    Anti-CD35 antibody [CR1/802] - BSA and Azide free
    See all CD35 primary antibodies
  • Description

    Mouse monoclonal [CR1/802] to CD35 - BSA and Azide free
  • Host species

    Mouse
  • Specificity

    ab212311 is highly specific to CD35 (CR1) and shows no cross-reaction with CD21 (CR2). ab212311 does not block CD35 (CR1) activity.
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, Monkey, Baboon
  • Immunogen

    Recombinant full length protein corresponding to Human CD35.
    Database link: P17927

  • Positive control

    • Human tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab212311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 2 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.

(in 0.1 ml).

IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(Primary incubation for 30 minutes at RT).

Target

  • Function

    Mediates cellular binding of particles and immune complexes that have activated complement.
  • Tissue specificity

    Present on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells.
  • Sequence similarities

    Belongs to the receptors of complement activation (RCA) family.
    Contains 30 Sushi (CCP/SCR) domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C3 binding protein antibody
    • C3b/C4b receptor antibody
    • C3BR antibody
    • C4BR antibody
    • CD 35 antibody
    • CD35 antibody
    • CD35 antigen antibody
    • complement component (3b/4b) receptor 1 (Knops blood group) antibody
    • complement component (3b/4b) receptor 1 including Knops blood group system antibody
    • Complement component receptor 1 antibody
    • Complement receptor 1 antibody
    • Complement receptor type 1 antibody
    • CR 1 antibody
    • CR1 antibody
    • CR1_HUMAN antibody
    • KN antibody
    • Knops blood group antigen antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD35 with ab212311 at 1 µg/ml.

References

ab212311 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab212311.
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