• Product name
    Anti-CD35 antibody [E11]
    See all CD35 primary antibodies
  • Description
    Mouse monoclonal [E11] to CD35
  • Host species
  • Specificity
    Does not block complement receptor 1 (CR1) activity. No cross reaction with CR2.
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-P, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Human, Baboon, Cynomolgus monkey, Rhesus monkey
  • Immunogen

    Tissue, cells or virus corresponding to CD35. human acute monocytic leukaemia cells.
    Database link: P17927



Our Abpromise guarantee covers the use of ab25 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 223 kDa.
Flow Cyt Use at an assay dependent concentration.

Use 10μl of the diluted antibody to label 106 cells or 100μl of whole blood





ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

acetone fixation


  • Function
    Mediates cellular binding of particles and immune complexes that have activated complement.
  • Tissue specificity
    Present on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells.
  • Sequence similarities
    Belongs to the receptors of complement activation (RCA) family.
    Contains 30 Sushi (CCP/SCR) domains.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • C3 binding protein antibody
    • C3b/C4b receptor antibody
    • C3BR antibody
    • C4BR antibody
    • CD 35 antibody
    • CD35 antibody
    • CD35 antigen antibody
    • complement component (3b/4b) receptor 1 (Knops blood group) antibody
    • complement component (3b/4b) receptor 1 including Knops blood group system antibody
    • Complement component receptor 1 antibody
    • Complement receptor 1 antibody
    • Complement receptor type 1 antibody
    • CR 1 antibody
    • CR1 antibody
    • CR1_HUMAN antibody
    • KN antibody
    • Knops blood group antigen antibody
    see all


  • ab25 (1µg/ml) staining CD35 in human spleen.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.


This product has been referenced in:
  • Fonseca MI  et al. Analysis of the Putative Role of CR1 in Alzheimer's Disease: Genetic Association, Expression and Function. PLoS One 11:e0149792 (2016). Read more (PubMed: 26914463) »
  • Park HJ  et al. Using mutagenesis and structural biology to map the binding site for the Plasmodium falciparum merozoite protein PfRh4 on the human immune adherence receptor. J Biol Chem 289:450-63 (2014). ELISA . Read more (PubMed: 24214979) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for contacting us.

Only the BSA seems to be a problem, its concentration should be 0.1%-0.5%. The ideal way is to remove BSA, we have kit for this https://www.abcam.com/BSA-Removal-Kit-ab173231.html. Alternatively you can give it a go however it might affect the efficiency of the kit. Please check the information on booklet;

10.2 Common non-buffering salts (e.g. sodium chloride), chelating agents (e.g.EDTA), and sugars have no effect on conjugation efficiency. Azide (0.02 to 0.1%) and BSA (0.1 to 0.5%) have little or no effect. Glycerol up to 50% has no effect.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.


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Thank you for contacting us. Samples for this kit have to be prepared as follows: tissues or cells can be homogenized in 4 volumes of Assay Buffer and centrifuged (13,000 x g, 10 min) to remove insoluble material. Serum samples can be directly diluted in the Assay Buffer. Prepare test samples of up to 50 μl/well with Assay Buffer in a 96-well plate. We suggest testing several doses of your sample to make sure the readings are within the standard curve range.”We recommend that the lysates are prepared in the assay buffer of the kit as it is most compatible. Theoretically though I do no not foresee any issues in theyour sample type and sample buffer, however this will have to be optimized by the end user.

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