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    cd35-antibody-e11-bsa-and-azide-free-ab25.pdf

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Immunology Cell Type Markers CD Myeloid Cells
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Anti-CD35 antibody [E11] - BSA and Azide free (ab25)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (6)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 [E11] antibody - BSA and Azide free (ab25)

    Key features and details

    • Mouse monoclonal [E11] to CD35 - BSA and Azide free
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-CD35 antibody [E11] - BSA and Azide free
      See all CD35 primary antibodies
    • Description

      Mouse monoclonal [E11] to CD35 - BSA and Azide free
    • Host species

      Mouse
    • Specificity

      Does not block complement receptor 1 (CR1) activity. No cross reaction with CR2.
    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data
    • Immunogen

      Tissue, cells or virus corresponding to CD35. human acute monocytic leukaemia cells.
      Database link: P17927

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Constituent: PBS
    • Carrier free

      Yes
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      E11
    • Myeloma

      P3-NS1/1-Ag4-1
    • Isotype

      IgG
    • Light chain type

      unknown
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Myeloid Cells
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Dendritic Cell Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Neutrophil Lineage

    Associated products

    • Alternative Versions

      • PE Anti-CD35 antibody [E11] (ab178451)
      • APC Anti-CD35 antibody [E11] (ab243085)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab25 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    Application Abreviews Notes
    IHC-P
    Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    Notes
    IHC-P
    Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    • Function

      Mediates cellular binding of particles and immune complexes that have activated complement.
    • Tissue specificity

      Present on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells.
    • Sequence similarities

      Belongs to the receptors of complement activation (RCA) family.
      Contains 30 Sushi (CCP/SCR) domains.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P17927 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1378 Human
      • Omim: 120620 Human
      • SwissProt: P17927 Human
      • Unigene: 334019 Human
      • Alternative names

        • C3 binding protein antibody
        • C3b/C4b receptor antibody
        • C3BR antibody
        • C4BR antibody
        • CD 35 antibody
        • CD35 antibody
        • CD35 antigen antibody
        • complement component (3b/4b) receptor 1 (Knops blood group) antibody
        • complement component (3b/4b) receptor 1 including Knops blood group system antibody
        • Complement component receptor 1 antibody
        • Complement receptor 1 antibody
        • Complement receptor type 1 antibody
        • CR 1 antibody
        • CR1 antibody
        • CR1_HUMAN antibody
        • KN antibody
        • Knops blood group antigen antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 [E11] antibody - BSA and Azide free (ab25)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 [E11] antibody - BSA and Azide free (ab25)
        ab25 (1µg/ml) staining CD35 in human spleen.
        Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (6)

      Publishing research using ab25? Please let us know so that we can cite the reference in this datasheet.

      ab25 has been referenced in 6 publications.

      • Yuan K  et al. Complement C3 overexpression activates JAK2/STAT3 pathway and correlates with gastric cancer progression. J Exp Clin Cancer Res 39:9 (2020). PubMed: 31928530
      • Fonseca MI  et al. Analysis of the Putative Role of CR1 in Alzheimer's Disease: Genetic Association, Expression and Function. PLoS One 11:e0149792 (2016). PubMed: 26914463
      • Fetica B  et al. High prevalence of viral hepatitis in a series of splenic marginal zone lymphomas from Romania. Blood Cancer J 6:e498 (2016). PubMed: 27834940
      • Park HJ  et al. Using mutagenesis and structural biology to map the binding site for the Plasmodium falciparum merozoite protein PfRh4 on the human immune adherence receptor. J Biol Chem 289:450-63 (2014). ELISA . PubMed: 24214979
      • LaPan P  et al. Optimization of total protein and activity assays for the detection of MMP-12 in induced human sputum. BMC Pulm Med 10:40 (2010). ELISA ; Human . PubMed: 20678199
      • Hogg N  et al. Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1). Eur J Immunol 14:236-43 (1984). PubMed: 6368248

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Question

      I have recently purchased (ab188288 – Cy5 Fast Conjugation Kit) to label an Antibody for a flowcytometry. But the storage buffer of the antibody contains 1XPBS, 1%BSA, 20% Glycerol (pH7). And 0.01% Thimerosal was added as a preservative, would this has an effect on the conjugation. Should I do dialysis for the antibody before labelling?

      Read More

      Abcam community

      Verified customer

      Asked on Mar 18 2015

      Answer

      Thank you for contacting us.

      Only the BSA seems to be a problem, its concentration should be 0.1%-0.5%. The ideal way is to remove BSA, we have kit for this https://www.abcam.com/BSA-Removal-Kit-ab173231.html. Alternatively you can give it a go however it might affect the efficiency of the kit. Please check the information on booklet;

      10.2 Common non-buffering salts (e.g. sodium chloride), chelating agents (e.g.EDTA), and sugars have no effect on conjugation efficiency. Azide (0.02 to 0.1%) and BSA (0.1 to 0.5%) have little or no effect. Glycerol up to 50% has no effect.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      -----------------------------------------------------------------------------------------------

      Can’t find the antibody you need? Try our Custom RabMAb® services.

      Discover More at https://www.abcam.com/tag/custom%20services

      Read More

      Padamjeet Singh

      Abcam Scientific Support

      Answered on Mar 18 2015

      Question

      To whom it may concern,



      We are interested in purchasing your MPO kit (ab111749) but have a few technical questions prior to a decision:



      1. Is the kit compatible with samples in buffers containing triton X-100, Tween-20 and EDTA?

      2. Is the kit compatible with samples derived from organ/tissue homogenates containing protease inhibitors?





      Read More

      Abcam community

      Verified customer

      Asked on Mar 08 2012

      Answer

      Thank you for contacting us. Samples for this kit have to be prepared as follows: tissues or cells can be homogenized in 4 volumes of Assay Buffer and centrifuged (13,000 x g, 10 min) to remove insoluble material. Serum samples can be directly diluted in the Assay Buffer. Prepare test samples of up to 50 μl/well with Assay Buffer in a 96-well plate. We suggest testing several doses of your sample to make sure the readings are within the standard curve range.”We recommend that the lysates are prepared in the assay buffer of the kit as it is most compatible. Theoretically though I do no not foresee any issues in theyour sample type and sample buffer, however this will have to be optimized by the end user.

      Read More

      Abcam Scientific Support

      Answered on Mar 08 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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