Anti-CD35 antibody [E11] - BSA and Azide free (ab25)

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Only the BSA seems to be a problem, its concentration should be 0.1%-0.5%. The ideal way is to remove BSA, we have kit for this https://www.abcam.com/BSA-Removal-Kit-ab173231.html. Alternatively you can give it a go however it might affect the efficiency of the kit. Please check the information on booklet;

10.2 Common non-buffering salts (e.g. sodium chloride), chelating agents (e.g.EDTA), and sugars have no effect on conjugation efficiency. Azide (0.02 to 0.1%) and BSA (0.1 to 0.5%) have little or no effect. Glycerol up to 50% has no effect.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting us. Samples for this kit have to be prepared as follows: tissues or cells can be homogenized in 4 volumes of Assay Buffer and centrifuged (13,000 x g, 10 min) to remove insoluble material. Serum samples can be directly diluted in the Assay Buffer. Prepare test samples of up to 50 μl/well with Assay Buffer in a 96-well plate. We suggest testing several doses of your sample to make sure the readings are within the standard curve range.”We recommend that the lysates are prepared in the assay buffer of the kit as it is most compatible. Theoretically though I do no not foresee any issues in theyour sample type and sample buffer, however this will have to be optimized by the end user.