Anti-CD35 antibody [E11] - BSA and Azide free (ab25)
Key features and details
- Mouse monoclonal [E11] to CD35 - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD35 antibody [E11] - BSA and Azide free
See all CD35 primary antibodies -
Description
Mouse monoclonal [E11] to CD35 - BSA and Azide free -
Host species
Mouse -
Specificity
Does not block complement receptor 1 (CR1) activity. No cross reaction with CR2. -
Tested Applications & Species
Application Species IHC-P Human -
Immunogen
Tissue, cells or virus corresponding to CD35. human acute monocytic leukaemia cells.
Database link: P17927
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E11 -
Myeloma
P3-NS1/1-Ag4-1 -
Isotype
IgG -
Light chain type
unknown -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
IHC-P |
Human
|
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Mediates cellular binding of particles and immune complexes that have activated complement. -
Tissue specificity
Present on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. -
Sequence similarities
Belongs to the receptors of complement activation (RCA) family.
Contains 30 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1378 Human
- Omim: 120620 Human
- SwissProt: P17927 Human
- Unigene: 334019 Human
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Alternative names
- C3 binding protein antibody
- C3b/C4b receptor antibody
- C3BR antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 [E11] antibody - BSA and Azide free (ab25)ab25 (1µg/ml) staining CD35 in human spleen.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References (6)
ab25 has been referenced in 6 publications.
- Yuan K et al. Complement C3 overexpression activates JAK2/STAT3 pathway and correlates with gastric cancer progression. J Exp Clin Cancer Res 39:9 (2020). PubMed: 31928530
- Fonseca MI et al. Analysis of the Putative Role of CR1 in Alzheimer's Disease: Genetic Association, Expression and Function. PLoS One 11:e0149792 (2016). PubMed: 26914463
- Fetica B et al. High prevalence of viral hepatitis in a series of splenic marginal zone lymphomas from Romania. Blood Cancer J 6:e498 (2016). PubMed: 27834940
- Park HJ et al. Using mutagenesis and structural biology to map the binding site for the Plasmodium falciparum merozoite protein PfRh4 on the human immune adherence receptor. J Biol Chem 289:450-63 (2014). ELISA . PubMed: 24214979
- LaPan P et al. Optimization of total protein and activity assays for the detection of MMP-12 in induced human sputum. BMC Pulm Med 10:40 (2010). ELISA ; Human . PubMed: 20678199
- Hogg N et al. Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1). Eur J Immunol 14:236-43 (1984). PubMed: 6368248