PE Anti-CD35 antibody [E11] (ab178451)
Key features and details
- PE Mouse monoclonal [E11] to CD35
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
PE Anti-CD35 antibody [E11]
See all CD35 primary antibodies -
Description
PE Mouse monoclonal [E11] to CD35 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
This antibody recognizes CD35, a type I glycoprotein expressed on granulocytes, monocytes, B cells, folicular dendritic cells, erythrocytes, NK and T cell subsets, as well as e.g. on glomerulal podocytes. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates -
Immunogen
Tissue, cells or virus corresponding to Human CD35. Acute monocytic leukemia cells and normal blood monocytes
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Positive control
- Flow Cyt: Human blood.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA
BSA is high-grade and protease free. -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
E11 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
Target
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Function
Mediates cellular binding of particles and immune complexes that have activated complement. -
Tissue specificity
Present on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. -
Sequence similarities
Belongs to the receptors of complement activation (RCA) family.
Contains 30 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1378 Human
- Omim: 120620 Human
- SwissProt: P17927 Human
- Unigene: 334019 Human
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Alternative names
- C3 binding protein antibody
- C3b/C4b receptor antibody
- C3BR antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab178451 has been referenced in 1 publication.
- Byrnes C et al. Identification of a cross-reacting, monoclonal anti-human CD233 antibody for identification and sorting of rhesus macaque erythrocytes. Cytometry A 81:165-8 (2012). PubMed: 22170815