Overview

  • Product name

    Anti-CD35 antibody [EPR6601]
    See all CD35 primary antibodies
  • Description

    Rabbit monoclonal [EPR6601] to CD35
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in the extracellular domain of human CD35. (P17927).

  • Positive control

    • TF-1 cells, Human tonsil, Human kidney tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 223 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Mediates cellular binding of particles and immune complexes that have activated complement.
    • Tissue specificity

      Present on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells.
    • Sequence similarities

      Belongs to the receptors of complement activation (RCA) family.
      Contains 30 Sushi (CCP/SCR) domains.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • C3 binding protein antibody
      • C3b/C4b receptor antibody
      • C3BR antibody
      • C4BR antibody
      • CD 35 antibody
      • CD35 antibody
      • CD35 antigen antibody
      • complement component (3b/4b) receptor 1 (Knops blood group) antibody
      • complement component (3b/4b) receptor 1 including Knops blood group system antibody
      • Complement component receptor 1 antibody
      • Complement receptor 1 antibody
      • Complement receptor type 1 antibody
      • CR 1 antibody
      • CR1 antibody
      • CR1_HUMAN antibody
      • KN antibody
      • Knops blood group antigen antibody
      see all

    Images

    • All lanes : Anti-CD35 antibody [EPR6601] (ab133293) at 1/1000 dilution

      Lane 1 : TF-1 cell lysate
      Lane 2 : human tonsil lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 223 kDa
      Observed band size: 280 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling CD35 with ab133293 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD35 with 1/100 ab133293.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Smolarz M  et al. Proteome Profiling of Exosomes Purified from a Small Amount of Human Serum: The Problem of Co-Purified Serum Components. Proteomes 7:N/A (2019). Read more (PubMed: 31035355) »
    • Pesciotta EN  et al. Dysferlin and other non-red cell proteins accumulate in the red cell membrane of Diamond-Blackfan Anemia patients. PLoS One 9:e85504 (2014). WB ; Human . Read more (PubMed: 24454878) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Question
    Answer

    The Wester blot was performed using reducing conditions.

    Read More

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