Product nameAnti-CD35 antibody [EPR6601]
See all CD35 primary antibodies
DescriptionRabbit monoclonal [EPR6601] to CD35
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Human
A synthetic peptide corresponding to residues in the extracellular domain of human CD35. (P17927).
- TF-1 cells, Human tonsil, Human kidney tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C.
Dissociation constant (KD)KD = 5.70 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 223 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMediates cellular binding of particles and immune complexes that have activated complement.
Tissue specificityPresent on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells.
Sequence similaritiesBelongs to the receptors of complement activation (RCA) family.
Contains 30 Sushi (CCP/SCR) domains.
- Information by UniProt
- C3 binding protein antibody
- C3b/C4b receptor antibody
- C3BR antibody
All lanes : Anti-CD35 antibody [EPR6601] (ab133293) at 1/1000 dilution
Lane 1 : TF-1 cell lysate
Lane 2 : human tonsil lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 223 kDa
Observed band size: 280 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling CD35 with ab133293 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD35 with 1/100 ab133293.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Smolarz M et al. Proteome Profiling of Exosomes Purified from a Small Amount of Human Serum: The Problem of Co-Purified Serum Components. Proteomes 7:N/A (2019). Read more (PubMed: 31035355) »
- Pesciotta EN et al. Dysferlin and other non-red cell proteins accumulate in the red cell membrane of Diamond-Blackfan Anemia patients. PLoS One 9:e85504 (2014). WB ; Human . Read more (PubMed: 24454878) »