Overview

  • Product name
    Anti-CD35 antibody [EPR6602]
    See all CD35 primary antibodies
  • Description
    Rabbit monoclonal [EPR6602] to CD35
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD35 aa 2000-2100 (C terminal). The exact sequence is proprietary.

  • Positive control
    • TF1 and Human tonsil lysates; Human kidney and tonsil tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 223 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function
      Mediates cellular binding of particles and immune complexes that have activated complement.
    • Tissue specificity
      Present on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells.
    • Sequence similarities
      Belongs to the receptors of complement activation (RCA) family.
      Contains 30 Sushi (CCP/SCR) domains.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
      • Alternative names
        • C3 binding protein antibody
        • C3b/C4b receptor antibody
        • C3BR antibody
        • C4BR antibody
        • CD 35 antibody
        • CD35 antibody
        • CD35 antigen antibody
        • complement component (3b/4b) receptor 1 (Knops blood group) antibody
        • complement component (3b/4b) receptor 1 including Knops blood group system antibody
        • Complement component receptor 1 antibody
        • Complement receptor 1 antibody
        • Complement receptor type 1 antibody
        • CR 1 antibody
        • CR1 antibody
        • CR1_HUMAN antibody
        • KN antibody
        • Knops blood group antigen antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] (ab126737)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] (ab126737)
        ab126737 at 1/250 dilution, staining CD35 in Formalin fixed Paraffin-embedded Human tonsil tissue by Immunohistochemistry.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] (ab126737)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] (ab126737)
        ab126737 at 1/250 dilution, staining CD35 in Formalin fixed Paraffin-embedded Human kidney tissue by Immunohistochemistry.
      • Western blot - Anti-CD35 antibody [EPR6602] (ab126737)
        Western blot - Anti-CD35 antibody [EPR6602] (ab126737)
        All lanes : Anti-CD35 antibody [EPR6602] (ab126737) at 1/1000 dilution

        Lane 1 : TF1 lysate
        Lane 2 : Human tonsil lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 223 kDa

      References

      This product has been referenced in:
      • Willcox A  et al. Germinal centre frequency is decreased in pancreatic lymph nodes from individuals with recent-onset type 1 diabetes. Diabetologia 60:1294-1303 (2017). Human . Read more (PubMed: 28213757) »
      • Ohe R  et al. Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues. Leuk Lymphoma 57:1687-96 (2016). IHC ; Human . Read more (PubMed: 26700650) »

      See all 2 Publications for this product

      Publishing research using ab126737? Please let us know so that we can cite the reference in this datasheet.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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