Product nameAnti-CD35 antibody [EPR6602]
See all CD35 primary antibodies
DescriptionRabbit monoclonal [EPR6602] to CD35
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide within Human CD35 aa 2000-2100 (C terminal). The exact sequence is proprietary.
- TF1 and Human tonsil lysates; Human kidney and tonsil tissues.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab126737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 223 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||1/250 - 1/500.|
FunctionMediates cellular binding of particles and immune complexes that have activated complement.
Tissue specificityPresent on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells.
Sequence similaritiesBelongs to the receptors of complement activation (RCA) family.
Contains 30 Sushi (CCP/SCR) domains.
- Information by UniProt
- C3 binding protein antibody
- C3b/C4b receptor antibody
- C3BR antibody
ab126737 at 1/250 dilution, staining CD35 in Formalin fixed Paraffin-embedded Human tonsil tissue by Immunohistochemistry.
ab126737 at 1/250 dilution, staining CD35 in Formalin fixed Paraffin-embedded Human kidney tissue by Immunohistochemistry.
All lanes : Anti-CD35 antibody [EPR6602] (ab126737) at 1/1000 dilution
Lane 1 : TF1 lysate
Lane 2 : Human tonsil lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 223 kDa
This product has been referenced in:
- Willcox A et al. Germinal centre frequency is decreased in pancreatic lymph nodes from individuals with recent-onset type 1 diabetes. Diabetologia 60:1294-1303 (2017). Human . Read more (PubMed: 28213757) »
- Ohe R et al. Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues. Leuk Lymphoma 57:1687-96 (2016). IHC ; Human . Read more (PubMed: 26700650) »