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    cd35-antibody-epr6602-ab126737.pdf

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Immunology Cell Type Markers CD Myeloid Cells
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RecombinantRabMAb

Recombinant Anti-CD35 antibody [EPR6602] (ab126737)

  • Datasheet
Submit a review Submit a question References (3)

Product price, shipping and contact information

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] (ab126737)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] (ab126737)
  • Western blot - Anti-CD35 antibody [EPR6602] (ab126737)
  • Anti-CD35 antibody [EPR6602] (ab126737)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6602] to CD35
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

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Overview

  • Product name

    Anti-CD35 antibody [EPR6602]
    See all CD35 primary antibodies
  • Description

    Rabbit monoclonal [EPR6602] to CD35
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human CD35 aa 2000-2100 (C terminal). The exact sequence is proprietary.

  • Positive control

    • TF1 and Human tonsil lysates; Human kidney and tonsil tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6602
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Myeloid Cells
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage

Associated products

  • Alternative Versions

    • Anti-CD35 antibody [EPR6602] - BSA and Azide free (ab248156)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 223 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 223 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Mediates cellular binding of particles and immune complexes that have activated complement.
    • Tissue specificity

      Present on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells.
    • Sequence similarities

      Belongs to the receptors of complement activation (RCA) family.
      Contains 30 Sushi (CCP/SCR) domains.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P17927 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1378 Human
      • Omim: 120620 Human
      • SwissProt: P17927 Human
      • Unigene: 334019 Human
      • Alternative names

        • C3 binding protein antibody
        • C3b/C4b receptor antibody
        • C3BR antibody
        • C4BR antibody
        • CD 35 antibody
        • CD35 antibody
        • CD35 antigen antibody
        • complement component (3b/4b) receptor 1 (Knops blood group) antibody
        • complement component (3b/4b) receptor 1 including Knops blood group system antibody
        • Complement component receptor 1 antibody
        • Complement receptor 1 antibody
        • Complement receptor type 1 antibody
        • CR 1 antibody
        • CR1 antibody
        • CR1_HUMAN antibody
        • KN antibody
        • Knops blood group antigen antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] (ab126737)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] (ab126737)

        ab126737 at 1/250 dilution, staining CD35 in Formalin fixed Paraffin-embedded Human tonsil tissue by Immunohistochemistry.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] (ab126737)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] (ab126737)

        ab126737 at 1/250 dilution, staining CD35 in Formalin fixed Paraffin-embedded Human kidney tissue by Immunohistochemistry.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-CD35 antibody [EPR6602] (ab126737)
        Western blot - Anti-CD35 antibody [EPR6602] (ab126737)
        All lanes : Anti-CD35 antibody [EPR6602] (ab126737) at 1/1000 dilution

        Lane 1 : TF1 lysate
        Lane 2 : Human tonsil lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 223 kDa

      • Anti-CD35 antibody [EPR6602] (ab126737)
        Anti-CD35 antibody [EPR6602] (ab126737)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (3)

      Publishing research using ab126737? Please let us know so that we can cite the reference in this datasheet.

      ab126737 has been referenced in 3 publications.

      • Jackson HM  et al. A novel mouse model expressing human forms for complement receptors CR1 and CR2. BMC Genet 21:101 (2020). PubMed: 32907542
      • Willcox A  et al. Germinal centre frequency is decreased in pancreatic lymph nodes from individuals with recent-onset type 1 diabetes. Diabetologia 60:1294-1303 (2017). Human . PubMed: 28213757
      • Ohe R  et al. Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues. Leuk Lymphoma 57:1687-96 (2016). IHC ; Human . PubMed: 26700650

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab126737.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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