Recombinant Anti-CD35 antibody [EPR6602] (ab126737)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-CD35 antibody [EPR6602]
See all CD35 primary antibodies -
Description
Rabbit monoclonal [EPR6602] to CD35 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD35 aa 2000-2100 (C terminal). The exact sequence is proprietary.
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Positive control
- TF1 and Human tonsil lysates; Human kidney and tonsil tissues.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6602 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab126737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 223 kDa). | |
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC | 1/250 - 1/500. |
Target
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Function
Mediates cellular binding of particles and immune complexes that have activated complement. -
Tissue specificity
Present on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. -
Sequence similarities
Belongs to the receptors of complement activation (RCA) family.
Contains 30 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1378 Human
- Omim: 120620 Human
- SwissProt: P17927 Human
- Unigene: 334019 Human
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Alternative names
- C3 binding protein antibody
- C3b/C4b receptor antibody
- C3BR antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] (ab126737)](https://www.abcam.com/ps/products/126/ab126737/Images/ab126737-140721-anti-cd35-antibody-epr6602-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] (ab126737)ab126737 at 1/250 dilution, staining CD35 in Formalin fixed Paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] (ab126737)](https://www.abcam.com/ps/products/126/ab126737/Images/ab126737-140722-anti-cd35-antibody-epr6602-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] (ab126737)ab126737 at 1/250 dilution, staining CD35 in Formalin fixed Paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-CD35 antibody [EPR6602] (ab126737)](https://www.abcam.com/ps/products/126/ab126737/Images/ab126737-140723-anti-cd35-antibody-epr6602-western-blot.jpg)
Western blot - Anti-CD35 antibody [EPR6602] (ab126737)All lanes : Anti-CD35 antibody [EPR6602] (ab126737) at 1/1000 dilution
Lane 1 : TF1 lysate
Lane 2 : Human tonsil lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 223 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Willcox A et al. Germinal centre frequency is decreased in pancreatic lymph nodes from individuals with recent-onset type 1 diabetes. Diabetologia 60:1294-1303 (2017). Human . Read more (PubMed: 28213757) »
- Ohe R et al. Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues. Leuk Lymphoma 57:1687-96 (2016). IHC ; Human . Read more (PubMed: 26700650) »
