Recombinant Anti-CD35 antibody [SP191] (ab227666)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP191] to CD35
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD35 antibody [SP191]
See all CD35 primary antibodies -
Description
Rabbit monoclonal [SP191] to CD35 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tonsil, kidney, colon and stomach adenocarcinoma tissue.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP191 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100.
Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling atroom temperature for 20 minutes. Incubate with primary antibody for 30 minutes at room temperature. |
Notes |
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IHC-P
1/100. Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling atroom temperature for 20 minutes. Incubate with primary antibody for 30 minutes at room temperature. |
Target
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Function
Mediates cellular binding of particles and immune complexes that have activated complement. -
Tissue specificity
Present on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. -
Sequence similarities
Belongs to the receptors of complement activation (RCA) family.
Contains 30 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1378 Human
- Omim: 120620 Human
- SwissProt: P17927 Human
- Unigene: 334019 Human
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Alternative names
- C3 binding protein antibody
- C3b/C4b receptor antibody
- C3BR antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD35 with ab227666 at 1100 dilution (2.69 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on germinal center of human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227666 for 30 mins at room temperature. -
Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD35 using ab227666 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon tissue stained for CD35 using ab227666 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for CD35 using ab227666 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD35 using ab227666 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227666 has not yet been referenced specifically in any publications.