Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP191] to CD35
- Suitable for: IHC-P
- Reacts with: Human
Product nameAnti-CD35 antibody [SP191]
See all CD35 primary antibodies
DescriptionRabbit monoclonal [SP191] to CD35
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CD35 aa 2000 to the C-terminus. The exact sequence is proprietary. Near the C-terminus.
Database link: P17927
- IHC-P: Human tonsil, kidney, colon and stomach adenocarcinoma tissue.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS by protein A/G.
Our Abpromise guarantee covers the use of ab227666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling atroom temperature for 20 minutes.
Incubate with primary antibody for 30 minutes at room temperature.
FunctionMediates cellular binding of particles and immune complexes that have activated complement.
Tissue specificityPresent on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells.
Sequence similaritiesBelongs to the receptors of complement activation (RCA) family.
Contains 30 Sushi (CCP/SCR) domains.
- Information by UniProt
- C3 binding protein antibody
- C3b/C4b receptor antibody
- C3BR antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD35 with ab227666 at 1100 dilution (2.69 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on germinal center of human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227666 for 30 mins at room temperature.
Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD35 using ab227666 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human colon tissue stained for CD35 using ab227666 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human kidney tissue stained for CD35 using ab227666 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD35 using ab227666 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227666 has not yet been referenced specifically in any publications.