Recombinant
RabMAb

Recombinant Anti-CD35 antibody [SP191] - BSA and Azide free (ab236236)

Overview

  • Product name

    Anti-CD35 antibody [SP191] - BSA and Azide free
    See all CD35 primary antibodies
  • Description

    Rabbit monoclonal [SP191] to CD35 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD35. The exact sequence is proprietary. Near the C-terminus.
    Database link: P17927

  • Positive control

    • IHC-P: Human colon and Human tonsil tissue
  • General notes

    Ab236236 is the carrier-free version of ab227666. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236236 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Applications

Our Abpromise guarantee covers the use of ab236236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling atroom temperature for 20 minutes. Incubate with primary antibody for 30 minutes at room temperature.

Target

  • Function

    Mediates cellular binding of particles and immune complexes that have activated complement.
  • Tissue specificity

    Present on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells.
  • Sequence similarities

    Belongs to the receptors of complement activation (RCA) family.
    Contains 30 Sushi (CCP/SCR) domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C3 binding protein antibody
    • C3b/C4b receptor antibody
    • C3BR antibody
    • C4BR antibody
    • CD 35 antibody
    • CD35 antibody
    • CD35 antigen antibody
    • complement component (3b/4b) receptor 1 (Knops blood group) antibody
    • complement component (3b/4b) receptor 1 including Knops blood group system antibody
    • Complement component receptor 1 antibody
    • Complement receptor 1 antibody
    • Complement receptor type 1 antibody
    • CR 1 antibody
    • CR1 antibody
    • CR1_HUMAN antibody
    • KN antibody
    • Knops blood group antigen antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD35 with ab227666 at 1/100 dilution (2.69 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on germinal center of human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227666 for 30 mins at room temperature. This image was generated using ab227666, the same clone, but with a different buffer formulation.

  • Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD35 using ab227666 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227666).

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for CD35 using ab227666 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227666).

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD35 using ab227666 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227666).

  • Formalin-fixed, paraffin-embedded human colon tissue stained for CD35 using ab227666 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227666).

References

ab236236 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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