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    cd36-antibody-ab124515.pdf

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Cardiovascular Blood Platelets
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Anti-CD36 antibody (ab124515)

  • Datasheet
Reviews (2) Submit a question References (3)

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Western blot - Anti-CD36 antibody (ab124515)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody (ab124515)
  • Western blot - Anti-CD36 antibody (ab124515)

Key features and details

  • Rabbit polyclonal to CD36
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human CD36 protein (His tag) (ab167735)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CD36 antibody
    See all CD36 primary antibodies
  • Description

    Rabbit polyclonal to CD36
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse CD36 aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab150445)

  • Positive control

    • This antibody gave a positive signal within WB in the following Mouse tissue lysates: Heart; P7 Adipose; Brown Adipose as well as Mouse spleen formalin fixed paraffin embedded tissue section within IHC-P.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Immunology
    • Cell Type Markers
    • CD
    • Platelets
    • Microbiology
    • Protein
    • Human Protein
    • Defensin
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Cardiovascular
    • Atherosclerosis
    • Lipid transport
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Heart disease
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human CD36 protein (His tag) (ab167735)

Applications

Our Abpromise guarantee covers the use of ab124515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 88 kDa (predicted molecular weight: 53 kDa).

Target

  • Function

    Multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity) (PubMed:18353783, PubMed:21610069). In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity) (PubMed:18753675). Involved in oral fat perception and preferences (PubMed:22240721, PubMed:25822988). Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellulare calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity) (PubMed:20037584). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity) (PubMed:16880211).
    (Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling.
  • Involvement in disease

    Platelet glycoprotein IV deficiency
    Coronary heart disease 7
  • Sequence similarities

    Belongs to the CD36 family.
  • Post-translational
    modifications

    N-glycosylated and O-glycosylated with a ratio of 2:1.
    Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome (PubMed:21610069, PubMed:18353783). Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids (PubMed:18353783).
  • Cellular localization

    Cell membrane. Membrane raft. Golgi apparatus. Apical cell membrane. Upon ligand-binding, internalized through dynamin-dependent endocytosis.
  • Target information above from: UniProt accession P16671 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 12491 Mouse
    • SwissProt: Q08857 Mouse
    • Unigene: 18628 Mouse
    • Unigene: 406799 Mouse
    • Alternative names

      • Adipocyte membrane protein antibody
      • BDPLT10 antibody
      • CD36 antibody
      • CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody
      • CD36 antigen antibody
      • CD36 molecule (thrombospondin receptor) antibody
      • CD36 molecule antibody
      • CD36_HUMAN antibody
      • CHDS7 antibody
      • Cluster determinant 36 antibody
      • Collagen receptor, platelet antibody
      • FAT antibody
      • Fatty acid translocase antibody
      • Fatty acid transport protein antibody
      • Glycoprotein IIIb antibody
      • GP IIIb antibody
      • GP3B antibody
      • GP4 antibody
      • GPIIIB antibody
      • GPIV antibody
      • Leukocyte differentiation antigen CD36 antibody
      • MGC108510 antibody
      • MGC91634 antibody
      • PAS 4 protein antibody
      • PAS IV antibody
      • PAS-4 antibody
      • PASIV antibody
      • Platelet collagen receptor antibody
      • Platelet glycoprotein 4 antibody
      • Platelet glycoprotein IV antibody
      • scarb3 antibody
      • Scavenger receptor class B member 3 antibody
      • SR-B3 antibody
      • SRB3 antibody
      • Thrombospondin receptor antibody
      see all

    Images

    • Western blot - Anti-CD36 antibody (ab124515)
      Western blot - Anti-CD36 antibody (ab124515)
      All lanes : Anti-CD36 antibody (ab124515) at 1/5000 dilution

      Lane 1 : Mouse platelet tissue lysate with 5% NFDM/TBST
      Lane 2 : Mouse adipose tissue lysate with 5% NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 53 kDa
      Observed band size: 75 kDa
      why is the actual band size different from the predicted?


      Exposure time: 90 seconds
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody (ab124515)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody (ab124515)
      IHC image of ab124515 staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab124515 ,1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
    • Western blot - Anti-CD36 antibody (ab124515)
      Western blot - Anti-CD36 antibody (ab124515)
      All lanes : Anti-CD36 antibody (ab124515) at 1 µg/ml

      Lane 1 : Heart (Mouse) Tissue Lysate
      Lane 2 : P7 Adipose (Mouse) Tissue Lysate
      Lane 3 : Brown Adipose (Mouse) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 53 kDa
      Observed band size: 88 kDa why is the actual band size different from the predicted?
      Additional bands at: 150 kDa, 300 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 4 minutes


      CD36 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124515 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab124515? Please let us know so that we can cite the reference in this datasheet.

    ab124515 has been referenced in 3 publications.

    • Reichenbach N  et al. Inhibition of Stat3-mediated astrogliosis ameliorates pathology in an Alzheimer's disease model. EMBO Mol Med 11:N/A (2019). PubMed: 30617153
    • Sabol RA  et al. Leptin produced by obesity-altered adipose stem cells promotes metastasis but not tumorigenesis of triple-negative breast cancer in orthotopic xenograft and patient-derived xenograft models. Breast Cancer Res 21:67 (2019). PubMed: 31118047
    • Aryal B  et al. ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression. Nat Commun 7:12313 (2016). WB . PubMed: 27460411

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-CD36 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (mammary tumor)
    Permeabilization
    No
    Specification
    mammary tumor
    Blocking step
    ab64261 kit as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 12 2017

    Western blot abreview for Anti-CD36 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris Gel)
    Sample
    Mouse Tissue lysate - whole (Heart)
    Specification
    Heart
    Treatment
    Day 0 and day 7 post-MI
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Dr. Merry Lindsey

    Verified customer

    Submitted Sep 20 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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