Recombinant Anti-CD36 antibody [EPR22509-40] (ab252922)

Product name

Anti-CD36 antibody [EPR22509-40]
See all CD36 primary antibodies
Description

Rabbit monoclonal [EPR22509-40] to CD36
Host species

Rabbit
Specificity

CD36 expression is low in the normal brain (PMID: 27646002).
Tested applications

Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: U937 whole cell lysate; rat spleen and fat tissue lysates; human lung and placenta tissue lysates, mouse white fat, mouse brown fat, mouse spleen, mouse brain. IHC-P: Human spleen and hepatocellular carcinoma tissue; rat stomach tissue. Flow cyt: THP-1 cells. ICC/IF: U937
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22509-40
Isotype

IgG
Research areas

Alternative Versions
- Anti-CD36 antibody [EPR22509-40] - BSA and Azide free (ab255331)
Compatible Secondaries
Immunohistochemistry reagents
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human CD36 protein (His tag) (ab167735)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab252922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Detects a band of approximately 70-110 kDa (predicted molecular weight: 53 kDa).
IHC-P		1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC is recommend only for human and rat. Unsuitable for mouse.
ICC/IF		1/50. Unsuitable for mouse
Flow Cyt		1/50. Unsuitable for mouse.

Notes
WB 1/1000. Detects a band of approximately 70-110 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC is recommend only for human and rat. Unsuitable for mouse.
ICC/IF 1/50. Unsuitable for mouse
Flow Cyt 1/50. Unsuitable for mouse.

Application notes

Is unsuitable for IP.

Function

Multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity) (PubMed:18353783, PubMed:21610069). In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity) (PubMed:18753675). Involved in oral fat perception and preferences (PubMed:22240721, PubMed:25822988). Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellulare calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity) (PubMed:20037584). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity) (PubMed:16880211).
(Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling.
Involvement in disease

Platelet glycoprotein IV deficiency
Coronary heart disease 7
Sequence similarities

Belongs to the CD36 family.
Post-translational
modifications

N-glycosylated and O-glycosylated with a ratio of 2:1.
Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome (PubMed:21610069, PubMed:18353783). Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids (PubMed:18353783).
Cellular localization

Cell membrane. Membrane raft. Golgi apparatus. Apical cell membrane. Upon ligand-binding, internalized through dynamin-dependent endocytosis.
Target information above from: UniProt accession P16671 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 948 Human
- Entrez Gene: 12491 Mouse
- Entrez Gene: 29184 Rat
- Omim: 173510 Human
- SwissProt: P16671 Human
- SwissProt: Q08857 Mouse
- SwissProt: Q07969 Rat
- Unigene: 120949 Human
- Unigene: 18628 Mouse
- Unigene: 406799 Mouse
- Unigene: 102418 Rat
- Unigene: 203220 Rat
see all
Alternative names
- Adipocyte membrane protein antibody
- BDPLT10 antibody
- CD36 antibody
- CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody
- CD36 antigen antibody
- CD36 molecule (thrombospondin receptor) antibody
- CD36 molecule antibody
- CD36_HUMAN antibody
- CHDS7 antibody
- Cluster determinant 36 antibody
- Collagen receptor, platelet antibody
- FAT antibody
- Fatty acid translocase antibody
- Fatty acid transport protein antibody
- Glycoprotein IIIb antibody
- GP IIIb antibody
- GP3B antibody
- GP4 antibody
- GPIIIB antibody
- GPIV antibody
- Leukocyte differentiation antigen CD36 antibody
- MGC108510 antibody
- MGC91634 antibody
- PAS 4 protein antibody
- PAS IV antibody
- PAS-4 antibody
- PASIV antibody
- Platelet collagen receptor antibody
- Platelet glycoprotein 4 antibody
- Platelet glycoprotein IV antibody
- scarb3 antibody
- Scavenger receptor class B member 3 antibody
- SR-B3 antibody
- SRB3 antibody
- Thrombospondin receptor antibody
see all

Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)

All lanes : Anti-CD36 antibody [EPR22509-40] (ab252922) at 1/1000 dilution

Lane 1 : Mouse white fat tissue lysate
Lane 2 : Mouse brown fat tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Mouse brain tissue lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 53 kDa
Observed band size: 100,70 kDa why is the actual band size different from the predicted?

Exposure time: 81 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The molecular weight observed is due to glycosylation and consistent with what has been described in the literature (PMID:8798390, 15677505, 20637247, 8631821).
Low expression: brain (PMID: 27646002 )
This blot was developed using a high sensitivity ECL substrate.
Immunocytochemistry/ Immunofluorescence - Anti-CD36 antibody [EPR22509-40] (ab252922)

Immunofluorescent analysis of 80% Methanol fixed 0.1% TritonX-100 permeabilised U937 (Human histiocytic lymphoma monocyte) cells labelling CD36 with ab252922 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution. Confocal image showing membranous staining in U937 cells (Human histiocytic lymphoma monocyte) is observed. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain Alpha Tubulin at 1/200 dilution.The Nuclear counterstain is DAPI .

Secondary antibody only control: Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution.
Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)

All lanes : Anti-CD36 antibody [EPR22509-40] (ab252922) at 1/1000 dilution

Lane 1 : Rat spleen lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Predicted band size: 53 kDa
Observed band size: 70-110 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking buffer: 5% NFDM/TBST

Dilution buffer: 5% NFDM /TBST

CD36 expression is low in the normal brain (PMID:27646002)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (ab252922)

Immunohistochemical analysis of [paraffin-embedded human spleen tissue labeling CD36 with ab252922 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of human spleen (PMID:30047927,22777768) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Flow Cytometry - Anti-CD36 antibody [EPR22509-40] (ab252922)

Flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cells labeling CD36 with ab252922 at 1/50 dilution (red) compared with a Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

Goat Anti-Rabbit IgG Fc (Alexa Fluo^r® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)

All lanes : Anti-CD36 antibody [EPR22509-40] (ab252922) at 1000 cells

Lane 1 : Human placenta tissue lysate
Lane 2 : Human lung tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

Predicted band size: 53 kDa

Exposure time: 3 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

The molecular weight observed is due to glycosylation and consistent with what has been described in the literature (PMID:8798390,15677505,20637247).
Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)

All lanes : Anti-CD36 antibody [EPR22509-40] (ab252922) at 1/1000 dilution

Lane 1 : U937 (human histiocytic lymphoma cell line) whole cell lysate
Lane 2 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : Rat spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 53 kDa
Observed band size: 70-110 kDa why is the actual band size different from the predicted?

Exposure time: 8 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

Negative control: Ramos, Jurkat (PMID: 27956597,10744776)

The molecular weight observed is due to glycosylation and consistent with what has been described in the literature (PMID:8798390,15677505,20637247, 8631821).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (ab252922)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling CD36 with ab252922 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of rat stomach. (PMID:30047927,22777768) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (ab252922)

Immunohistochemical analysis of paraffin-embedded human hepatocellular tissue labeling CD36 with ab252922 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of human hepatocellular carcinoma (PMID:30047927,22777768) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Flow Cytometry - Anti-CD36 antibody [EPR22509-40] (ab252922)

Flow cytometric analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cell line labelling CD36 with ab252922 at 1/50 dilution (red) compared with Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.

Negative control: Ramos (PMID: 10744776).Gated on viable cells.
Flow Cytometry - Anti-CD36 antibody [EPR22509-40] (ab252922)

Flow cytometric analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling CD36 with ab252922 at 1/50 dilution (red) compared with a Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

Negative control: Ramos (PMID: 10744776).
Anti-CD36 antibody [EPR22509-40] (ab252922)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

Download

Publishing research using ab252922? Please let us know so that we can cite the reference in this datasheet.

ab252922 has been referenced in 1 publication.

Supruniuk E et al. Pyrroloquinoline Quinone Modifies Lipid Profile, but Not Insulin Sensitivity, of Palmitic Acid-Treated L6 Myotubes. Int J Mol Sci 21:N/A (2020). PubMed: 33171690

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Immunohistochemistry reagents

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (1)

Customer reviews and Q&As

Post-translational
modifications