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RecombinantRabMAb

Recombinant Anti-CD36 antibody [EPR6573] (ab133625)

  • Datasheet
  • SDS
Reviews (8) Submit a question References (67)

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Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR6573] (ab133625)
  • Immunoprecipitation - Anti-CD36 antibody [EPR6573] (ab133625)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR6573] (ab133625)
  • Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
  • Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
  • Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
  • Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
  • Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
  • Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
  • Immunoprecipitation - Anti-CD36 antibody [EPR6573] (ab133625)
  • Anti-CD36 antibody [EPR6573] (ab133625)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6573] to CD36
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-CD36 antibody [EPR6573]
    See all CD36 primary antibodies
  • Description

    Rabbit monoclonal [EPR6573] to CD36
  • Host species

    Rabbit
  • Specificity

    The immunogen used for this product shares 57% homology with SCARB1. Cross-reactivity with this protein has not been confirmed experimentally. Expression levels of the target protein vary with sample type and some optimisation may be required.

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Guinea pig
  • Immunogen

    Synthetic peptide within Human CD36 aa 350-450. The exact sequence is proprietary.
    Database link: P16671
    (Peptide available as ab190596)

  • Positive control

    • WB: HepG2, 3T3-L1 and NIH 3T3 cell lysates; human adipose tissue and platelet lysates. IP: 3T3-L1 cell lysate. IHC-P: FFPE Mouse small intestine tissue; human cardiac muscle and hepatocellular cancer tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6573
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Immunology
    • Cell Type Markers
    • CD
    • Platelets
    • Microbiology
    • Protein
    • Human Protein
    • Defensin
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Cardiovascular
    • Atherosclerosis
    • Lipid transport
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Heart disease
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Alternative Versions

    • Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • CD36 peptide (ab190596)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human CD36 protein (His tag) (ab167735)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (6)
1/1000 - 1/10000. Detects a band of approximately 78-88 kDa (predicted molecular weight: 53 kDa).Can be blocked with CD36 peptide (ab190596).

For unpurified use at 1/100 - 1/1000. DS: For Lysate preparation protocol, please refer to the protocol book in the protocol section.

Product runs at 75-85 kDa due to glycosylation.

IP (1)
1/50.

For unpurified use at 1/5.

IHC-P (1)
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

We do not guarantee IHC-P for mouse species and did not test IHC-P on guinea pig tissues.

Notes
WB
1/1000 - 1/10000. Detects a band of approximately 78-88 kDa (predicted molecular weight: 53 kDa).Can be blocked with CD36 peptide (ab190596).

For unpurified use at 1/100 - 1/1000. DS: For Lysate preparation protocol, please refer to the protocol book in the protocol section.

Product runs at 75-85 kDa due to glycosylation.

IP
1/50.

For unpurified use at 1/5.

IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

We do not guarantee IHC-P for mouse species and did not test IHC-P on guinea pig tissues.

Target

  • Function

    Multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity) (PubMed:18353783, PubMed:21610069). In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity) (PubMed:18753675). Involved in oral fat perception and preferences (PubMed:22240721, PubMed:25822988). Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellulare calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity) (PubMed:20037584). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity) (PubMed:16880211).
    (Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling.
  • Involvement in disease

    Platelet glycoprotein IV deficiency
    Coronary heart disease 7
  • Sequence similarities

    Belongs to the CD36 family.
  • Post-translational
    modifications

    N-glycosylated and O-glycosylated with a ratio of 2:1.
    Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome (PubMed:21610069, PubMed:18353783). Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids (PubMed:18353783).
  • Cellular localization

    Cell membrane. Membrane raft. Golgi apparatus. Apical cell membrane. Upon ligand-binding, internalized through dynamin-dependent endocytosis.
  • Target information above from: UniProt accession P16671 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 948 Human
    • Entrez Gene: 12491 Mouse
    • Omim: 173510 Human
    • SwissProt: P16671 Human
    • SwissProt: Q08857 Mouse
    • Unigene: 120949 Human
    • Unigene: 18628 Mouse
    • Unigene: 406799 Mouse
    • Alternative names

      • Adipocyte membrane protein antibody
      • BDPLT10 antibody
      • CD36 antibody
      • CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody
      • CD36 antigen antibody
      • CD36 molecule (thrombospondin receptor) antibody
      • CD36 molecule antibody
      • CD36_HUMAN antibody
      • CHDS7 antibody
      • Cluster determinant 36 antibody
      • Collagen receptor, platelet antibody
      • FAT antibody
      • Fatty acid translocase antibody
      • Fatty acid transport protein antibody
      • Glycoprotein IIIb antibody
      • GP IIIb antibody
      • GP3B antibody
      • GP4 antibody
      • GPIIIB antibody
      • GPIV antibody
      • Leukocyte differentiation antigen CD36 antibody
      • MGC108510 antibody
      • MGC91634 antibody
      • PAS 4 protein antibody
      • PAS IV antibody
      • PAS-4 antibody
      • PASIV antibody
      • Platelet collagen receptor antibody
      • Platelet glycoprotein 4 antibody
      • Platelet glycoprotein IV antibody
      • scarb3 antibody
      • Scavenger receptor class B member 3 antibody
      • SR-B3 antibody
      • SRB3 antibody
      • Thrombospondin receptor antibody
      see all

    Images

    • Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
      Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
      All lanes : Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution

      Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates with 5% NFDM/TBST
      Lane 2 : THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml PMA (Phorbol-12-myristate-13-acetate) for 72 hours whole cell lysates with 5% NFDM/TBST
      Lane 3 : Human adipose lysates with 5% NFDM/TBST
      Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 5 : 3T3-L1 (Mouse embryonic fibroblast) whole cell lysates with 5% NFDM/TBST
      Lane 6 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates with 5% NFDM/TBST
      Lane 7 : Mouse liver lysates with 5% NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 53 kDa
      Observed band size: 78 kDa why is the actual band size different from the predicted?


      Exposure time: 50 seconds


      The expression level of CD36 varies in different samples, and it could be upregulated by treatments such as PMA and Porphyromonas gingivalis (PMID: 8576181 and 27234131).

      RAW 264.7 and mouse liver are reported to be positive for CD36 by PMID: 26187465 and 26186589, but this antibody failed to detect clear signal in normal conditions.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR6573] (ab133625)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR6573] (ab133625)

      Ab133625 staining CD36 in paraffin embedded Human Hepatocellular cancer tissue sections by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). Tissue was counterstained with hematoxylin and heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/10,000 dilution (0.17µg/ml). A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Positive staining on endothelial cells in human hepatocellular cancer.

    • Immunoprecipitation - Anti-CD36 antibody [EPR6573] (ab133625)
      Immunoprecipitation - Anti-CD36 antibody [EPR6573] (ab133625)

      ab133625 (unpurified) at 1/5 immunoprecipitating CD36 in 3T3-L1 cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR6573] (ab133625)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR6573] (ab133625)

      Ab133625 staining CD36 in paraffin embedded Human cardiac muscle tissue sections by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). Tissue was counterstained with hematoxylin and heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/10,000 dilution (0.17µg/ml). A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Positive staining mainly on endothelial cells in human cardiac muscle.

    • Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
      Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
      All lanes : Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution (unpurified)

      Lane 1 : 3T3-L1 cell lysate
      Lane 2 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 53 kDa
      Observed band size: 78-88 kDa why is the actual band size different from the predicted?



      The lysate in this image is prepared by 1%SDS Hot Lysate buffer. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

    • Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
      Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
      All lanes : Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution

      Lane 1 : 3T3-L1 (Mouse embryonic fibroblast) whole cell lysates prepared in RIPA lysis method with 5% NFDM/TBST
      Lane 2 : 3T3-L1 (Mouse embryonic fibroblast) whole cell lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 53 kDa
      Observed band size: 78 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds
    • Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
      Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
      Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution + HEK293 (human embryonic kidney epithelial cell) transfected with His-tagged human CD36 (30aa-439aa) expression vector, whole cell lysate at 20 µg with 5% NFDM/TBST

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 53 kDa
      Observed band size: 74 kDa why is the actual band size different from the predicted?


      Exposure time: 3 seconds
    • Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
      Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
      All lanes : Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution

      Lane 1 : Human Heart Tissue Lysate
      Lane 2 : Human Adipose Tissue Lysate
      Lane 3 : Mouse Adipose Tissue Lysate
      Lane 4 : Human Platelet Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 53 kDa
      Observed band size: 88 kDa why is the actual band size different from the predicted?


      Exposure time: 2 minutes


      This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab133625 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    • Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
      Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
      Anti-CD36 antibody [EPR6573] (ab133625) at 1/10000 dilution (purified) + NIH/3T3 cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 53 kDa
      Observed band size: 78-88 kDa why is the actual band size different from the predicted?



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

      The lysate in this image is prepared by 1%SDS Hot lysis method.

      For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

    • Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
      Western blot - Anti-CD36 antibody [EPR6573] (ab133625)
      Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution (unpurified) + NIH/3T3 at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 53 kDa
      Observed band size: 78-88 kDa why is the actual band size different from the predicted?



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

      The lysate in this image is prepared by 1%SDS Hot Lysate buffer. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

    • Immunoprecipitation - Anti-CD36 antibody [EPR6573] (ab133625)
      Immunoprecipitation - Anti-CD36 antibody [EPR6573] (ab133625)

      ab133625 (purified) at 1/50 immunoprecipitating CD36 in 3T3-L1 cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-CD36 antibody [EPR6573] (ab133625)
      Anti-CD36 antibody [EPR6573] (ab133625)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (67)

    Publishing research using ab133625? Please let us know so that we can cite the reference in this datasheet.

    ab133625 has been referenced in 67 publications.

    • Meda C  et al. Hepatic ERa accounts for sex differences in the ability to cope with an excess of dietary lipids. Mol Metab 32:97-108 (2020). PubMed: 32029233
    • Ren Z  et al. Anti-glycolipid disorder effect of epigallocatechin-3-gallate on high-fat diet and STZ-induced T2DM in mice. Mol Med Rep 21:2475-2483 (2020). PubMed: 32236613
    • Zhang F  et al. Inhibition of USP14 suppresses the formation of foam cell by promoting CD36 degradation. J Cell Mol Med 24:3292-3302 (2020). PubMed: 31970862
    • Ou M  et al. Long non-coding RNA CDKN2B-AS1 contributes to atherosclerotic plaque formation by forming RNA-DNA triplex in the CDKN2B promoter. EBioMedicine 55:102694 (2020). PubMed: 32335370
    • Wang S  et al. Effects of High-Glucose and High-Fat Condition on Estrogen Receptor- and Sexual Precocity-Related Genes in GT1-7 Cells. Med Sci Monit 26:e922860 (2020). PubMed: 32451371
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD36 antibody [EPR6573]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 1.5% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: antigen retrieval
    Sample
    Mouse Tissue sections (Heart at day 0 and day 7 post-MI)
    Specification
    Heart at day 0 and day 7 post-MI
    Permeabilization
    No
    Fixative
    10% zinc formalin
    Read More

    Dr. Merry Lindsey

    Verified customer

    Submitted Oct 17 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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