Recombinant Anti-CD36 antibody [EPR6573] (ab133625)

The expression level of CD36 varies in different samples, and it could be upregulated by treatments such as PMA and Porphyromonas gingivalis (PMID: 8576181 and 27234131).

RAW 264.7 and mouse liver are reported to be positive for CD36 by PMID: 26187465 and 26186589, but this antibody failed to detect clear signal in normal conditions.

Ab133625 staining CD36 in paraffin embedded Human Hepatocellular cancer tissue sections by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). Tissue was counterstained with hematoxylin and heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/10,000 dilution (0.17µg/ml). A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Positive staining on endothelial cells in human hepatocellular cancer.

ab133625 (unpurified) at 1/5 immunoprecipitating CD36 in 3T3-L1 cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.

Ab133625 staining CD36 in paraffin embedded Human cardiac muscle tissue sections by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). Tissue was counterstained with hematoxylin and heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/10,000 dilution (0.17µg/ml). A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Positive staining mainly on endothelial cells in human cardiac muscle.

The lysate in this image is prepared by 1%SDS Hot Lysate buffer. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab133625 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.

The lysate in this image is prepared by 1%SDS Hot lysis method.

For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.

The lysate in this image is prepared by 1%SDS Hot Lysate buffer. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

ab133625 (purified) at 1/50 immunoprecipitating CD36 in 3T3-L1 cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.