Application
Blocking
Sample
Mouse Cell (mouse macrophage cell line(raw264.7 cell))
Specification
mouse macrophage cell line(raw264.7 cell)
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: DMEM
Dilution
1/10
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
We tested this Ab for blocking CD36 with mouse sample. Datasheet of this Ab shows that this have reactivity with rat and human, but we have a reference that it can have cross-reactivity between mouse and rat. So we treated palmitic acid (CD36 ligand) on RAW264.7 cell with and without this CD36 blocking Ab and checked TNF-a by ELISA. But we coundn't find that this FA6-152 Ab have cross-reactivity with mouse, at least for blocking CD36.
Abcam response
Thank you for your valuable feedback. ab17044 has not been previously tested in mouse sample. It is possible that this antibody may not be suitable for use in this species. We welcome other Abreviews to determine this.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Nov 27 2014