Overview

  • Product name
    Anti-CD36 antibody [JC63.1]
    See all CD36 primary antibodies
  • Description
    Mouse monoclonal [JC63.1] to CD36
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, Blocking, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Mouse CD36.
    Database link: Q08857

  • Positive control
    • Cells from adipose tissue, heart tissue, platelets and macrophages. Flow cytometry: RAW264.7 cells. ICC/IF: 3T3-L1 adipocytes.

Applications

Our Abpromise guarantee covers the use of ab23680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 0.5 µg/ml.

ab37322 - Mouse monoclonal IgA, is suitable for use as an isotype control with this antibody.

 

Blocking Use a concentration of 2 µg/ml. (or 1/500).Note: ab23680 inhibits CD36 binding of ox-LDL (macrophages) and rod puter segments (retinal pigment epithelial cells) and prevents foam cell formation of macrophages.
ICC/IF Use at an assay dependent concentration. PubMed: 22205027

Target

  • Function
    Multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity) (PubMed:18353783, PubMed:21610069). In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity) (PubMed:18753675). Involved in oral fat perception and preferences (PubMed:22240721, PubMed:25822988). Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellulare calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity) (PubMed:20037584). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity) (PubMed:16880211).
    (Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling.
  • Involvement in disease
    Platelet glycoprotein IV deficiency
    Coronary heart disease 7
  • Sequence similarities
    Belongs to the CD36 family.
  • Post-translational
    modifications
    N-glycosylated and O-glycosylated with a ratio of 2:1.
    Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome (PubMed:21610069, PubMed:18353783). Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids (PubMed:18353783).
  • Cellular localization
    Cell membrane. Membrane raft. Golgi apparatus. Apical cell membrane. Upon ligand-binding, internalized through dynamin-dependent endocytosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adipocyte membrane protein antibody
    • BDPLT10 antibody
    • CD36 antibody
    • CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody
    • CD36 antigen antibody
    • CD36 molecule (thrombospondin receptor) antibody
    • CD36 molecule antibody
    • CD36_HUMAN antibody
    • CHDS7 antibody
    • Cluster determinant 36 antibody
    • Collagen receptor, platelet antibody
    • FAT antibody
    • Fatty acid translocase antibody
    • Fatty acid transport protein antibody
    • Glycoprotein IIIb antibody
    • GP IIIb antibody
    • GP3B antibody
    • GP4 antibody
    • GPIIIB antibody
    • GPIV antibody
    • Leukocyte differentiation antigen CD36 antibody
    • MGC108510 antibody
    • MGC91634 antibody
    • PAS 4 protein antibody
    • PAS IV antibody
    • PAS-4 antibody
    • PASIV antibody
    • Platelet collagen receptor antibody
    • Platelet glycoprotein 4 antibody
    • Platelet glycoprotein IV antibody
    • scarb3 antibody
    • Scavenger receptor class B member 3 antibody
    • Thrombospondin receptor antibody
    see all

Images

  • ab23680 staining the CD36 in RAW 264.7 cells by Flow Cytometry. 

  • Immunofluorescence analysis of 3T3-L1 adipocytes, staining CD36 with ab23680.

    Cells were fixed with paraformaldehyde, permeabilized with 0.01% saponin and blocked with 1% BSA. Samples were incubated with primary antibody overnight at 4°C.

References

This product has been referenced in:
  • Luo Y  et al. Macrophagic CD146 promotes foam cell formation and retention during atherosclerosis. Cell Res 27:352-372 (2017). Read more (PubMed: 28084332) »
  • Wang SY  et al. Effects of initiating time and dosage ofPanax notoginsengon mucosal microvascular injury in experimental colitis. World J Gastroenterol 23:8308-8320 (2017). Read more (PubMed: 29307991) »
See all 15 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (RAW264.7)
Specification
RAW264.7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 19 2014

Question
Answer

Thank you for your inquiry.

The lab said that the suggested concentration is 2 ug/ml but that the incubation times will need to be determined by the individual investigator depending on the biomarkers they are measuring.

I performed a literature search and I have copied links to publications that have used this antibody for in vitro experiments. We would suggest you perform a further literature search to find publications for your experimental model.

http://www.sciencedirect.com/science/article/pii/S0165247811000381
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2785357/
I apologize that this information could not be more helpful, but I hope this is a good starting point for you. Please contact us with any other questions.

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Answer

Thank you for your inquiry To our knowledge, the epitope has not been mapped. I quickly searched the literature for this clone, but was unable to find any information about epitope mapping of this clone. The immunogen for this antibody was the full length protein. Because the antibody does block the activity of the receptor, it can safely be assumed that the epitope is extracellular. I hope this information is helpful. Please feel free to contact me if you have any further questions.

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Answer

Thank you for contacting Abcam. For ab23680, anti-CD36, i would recommend ab37322 mouse IgGA isotype control. If there is anything else I can help you with, please let me know.

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Question
Answer

Thank you for your enquiry. Below is the list of publications using this antibody I promised you. The publications probably will not mention Abcam by name, since they were written before we started carrying the antibody. Unfortunately as I mentioned on the phone, I don't have access to all of the journals to see which publications would be helpful to you. I apologize for that! Greenwalt et al. Blood 90(5), 1105-1115 (1992). Pearce et al. Circ. Res. 90, 844-849 (2002). Febbraio et al. J. Biol. Chem. 274, 19055-19062 (1999). Podrez et al. J. Clin. Invest. 105(8), 1095-1108 (2000). Febbraio et al. J. Clin. Invest. 105(8), 1049-1056 (2000). Febbraio et al. J. Clin. Invest. 108(6), 785-791 (2001). Han et al. J. Biol. Chem. 272(34), 21654-21659 (1997). Tontonoz et al. Cell 93, 241252 (1998). Navazo et al. J. Biol. Chem. 271(26), 15381-15385 (1996). Jimenez et al. Nature Med. 6(1), 41-48 (2000). Rutella et al. Haematologica 84, 419-424 (1999). Also, as I noted over the phone and just wanted to reiterate in writing, since this antibody is tested in Flow on human, mouse, and rat, we will guarantee that it works in this application. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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