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    cd36-antibody-mf3-low-endotoxin-azide-free-ab80080.pdf

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Cardiovascular Blood Platelets
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Anti-CD36 antibody [MF3] - Low endotoxin, Azide free (ab80080)

  • Datasheet
Reviews (3)Q&A (3)References (19)

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Flow Cytometry - Anti-CD36 antibody [MF3] - Low endotoxin, Azide free (ab80080)

    Key features and details

    • Rat monoclonal [MF3] to CD36 - Low endotoxin, Azide free
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Isotype: IgG2a

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    Overview

    • Product name

      Anti-CD36 antibody [MF3] - Low endotoxin, Azide free
      See all CD36 primary antibodies
    • Description

      Rat monoclonal [MF3] to CD36 - Low endotoxin, Azide free
    • Host species

      Rat
    • Specificity

      Antibodies produced by clone MF3 have been shown to inhibit IL4 induced thioglycollate-elicited peritoneal macrophage fusion and significantly block IL4/GM-CSF-induced bone-marrorw derived macrophage fusion.
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Tissue, cells or virus corresponding to Mouse CD36. IL4 treated murine thioglycollate-elicited peritoneal macrophages.

    • Positive control

      • Mouse peripheral blood platelets.
    • General notes

      Endotoxin Level <0.01EU/ug.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Constituent: PBS
    • Carrier free

      Yes
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      MF3
    • Myeloma

      Y3/Ag1.2.3
    • Isotype

      IgG2a
    • Research areas

      • Cardiovascular
      • Blood
      • Platelets
      • Immunology
      • Cell Type Markers
      • CD
      • Platelets
      • Microbiology
      • Protein
      • Human Protein
      • Defensin
      • Stem Cells
      • Hematopoietic Progenitors
      • Hematopoietic Stem Cells
      • Human Lineage Negative
      • Cardiovascular
      • Atherosclerosis
      • Lipid transport
      • Stem Cells
      • Endothelial Progenitors
      • Endothelial Markers
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of lipids and lipoproteins
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipid metabolism
      • Metabolism
      • Types of disease
      • Cancer
      • Metabolism
      • Types of disease
      • Heart disease
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
    • Recombinant Protein

      • Recombinant Human CD36 protein (His tag) (ab167735)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab80080 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use at an assay dependent concentration.
    Notes
    Flow Cyt
    Use at an assay dependent concentration.

    Target

    • Function

      Multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity) (PubMed:18353783, PubMed:21610069). In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity) (PubMed:18753675). Involved in oral fat perception and preferences (PubMed:22240721, PubMed:25822988). Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellulare calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity) (PubMed:20037584). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity) (PubMed:16880211).
      (Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling.
    • Involvement in disease

      Platelet glycoprotein IV deficiency
      Coronary heart disease 7
    • Sequence similarities

      Belongs to the CD36 family.
    • Post-translational
      modifications

      N-glycosylated and O-glycosylated with a ratio of 2:1.
      Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome (PubMed:21610069, PubMed:18353783). Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids (PubMed:18353783).
    • Cellular localization

      Cell membrane. Membrane raft. Golgi apparatus. Apical cell membrane. Upon ligand-binding, internalized through dynamin-dependent endocytosis.
    • Target information above from: UniProt accession P16671 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 12491 Mouse
      • SwissProt: Q08857 Mouse
      • Unigene: 18628 Mouse
      • Unigene: 406799 Mouse
      • Alternative names

        • Adipocyte membrane protein antibody
        • BDPLT10 antibody
        • CD36 antibody
        • CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody
        • CD36 antigen antibody
        • CD36 molecule (thrombospondin receptor) antibody
        • CD36 molecule antibody
        • CD36_HUMAN antibody
        • CHDS7 antibody
        • Cluster determinant 36 antibody
        • Collagen receptor, platelet antibody
        • FAT antibody
        • Fatty acid translocase antibody
        • Fatty acid transport protein antibody
        • Glycoprotein IIIb antibody
        • GP IIIb antibody
        • GP3B antibody
        • GP4 antibody
        • GPIIIB antibody
        • GPIV antibody
        • Leukocyte differentiation antigen CD36 antibody
        • MGC108510 antibody
        • MGC91634 antibody
        • PAS 4 protein antibody
        • PAS IV antibody
        • PAS-4 antibody
        • PASIV antibody
        • Platelet collagen receptor antibody
        • Platelet glycoprotein 4 antibody
        • Platelet glycoprotein IV antibody
        • scarb3 antibody
        • Scavenger receptor class B member 3 antibody
        • SR-B3 antibody
        • SRB3 antibody
        • Thrombospondin receptor antibody
        see all

      Images

      • Flow Cytometry - Anti-CD36 antibody [MF3] - Low endotoxin, Azide free (ab80080)
        Flow Cytometry - Anti-CD36 antibody [MF3] - Low endotoxin, Azide free (ab80080)

        Figure A. RPE conjugated Rat anti Mouse CD11b and Pacific Blue conjugated Rat IgG2a isotype control. Figure B. RPE conjugated Rat anti Mouse CD11b and Pacific Blue conjugated Rat anti Mouse CD36. All experiments performed on mouse peritoneal macrophages gated on live, single cells in the presence of 10% mouse serum. Data acquired on the ZE5™ Cell Analyzer

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (19)

      Publishing research using ab80080? Please let us know so that we can cite the reference in this datasheet.

      ab80080 has been referenced in 19 publications.

      • Yusuf B  et al. The neurorepellent, Slit2, prevents macrophage lipid loading by inhibiting CD36-dependent binding and internalization of oxidized low-density lipoprotein. Sci Rep 11:3614 (2021). PubMed: 33574432
      • Koronyo-Hamaoui M  et al. Peripherally derived angiotensin converting enzyme-enhanced macrophages alleviate Alzheimer-related disease. Brain 143:336-358 (2020). PubMed: 31794021
      • Yang Q  et al. Prkaa1 Metabolically Regulates Monocyte/Macrophage Recruitment and Viability in Diet-Induced Murine Metabolic Disorders. Front Cell Dev Biol 8:611354 (2020). PubMed: 33511118
      • Xie Z  et al. In vivo assessment of inflammation in carotid atherosclerosis by noninvasive photoacoustic imaging. Theranostics 10:4694-4704 (2020). PubMed: 32292523
      • Li S  et al. Activated Bone Marrow-Derived Macrophages Eradicate Alzheimer's-Related Aß42 Oligomers and Protect Synapses. Front Immunol 11:49 (2020). PubMed: 32082319
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-6 of 6 Abreviews or Q&A

      Immunocytochemistry abreview for Anti-CD36 antibody [MF3] - Low Endotoxin

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Mouse Cell (RAW 264.7)
      Specification
      RAW 264.7
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.1% Triton-X100 in 2% BSA for 15min
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Dr. Mahesh Shivananjappa

      Verified customer

      Submitted May 23 2012

      Western blot abreview for Anti-CD36 antibody [MF3] - Low Endotoxin

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Monocytes from Apheresis)
      Loading amount
      20 µg
      Specification
      Monocytes from Apheresis
      Gel Running Conditions
      Reduced Denaturing (8-16% Biorad Criterion Gel with 18 wells)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Dr. Mahesh Shivananjappa

      Verified customer

      Submitted Dec 12 2011

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD36 antibody [MF3] - Low Endotoxin

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (human coronary atherosclerosis plaques)
      Specification
      human coronary atherosclerosis plaques
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate buffer ph 6
      Permeabilization
      No
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 36°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Dr. Jaykumar Thumar

      Verified customer

      Submitted Dec 31 2010

      Question

      Thanks for your information about mouse CD36 antibody. I want to detect CD36 from c2c12 cell lines, a mouse cell lines. I just request you if you send me a little aliquot 10 microlitre,I will just check 1 exp, then I will order. I will need a good amount to order. Please let me know it is possible or not.

      Read More

      Abcam community

      Verified customer

      Asked on May 25 2012

      Answer

      Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

      We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

      If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

      Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

      To find out more about our Abreview system, please see the following link:

      https://www.abcam.com/abreviews

      I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

      Read More

      Abcam Scientific Support

      Answered on May 25 2012

      Question

      Have you CD36 mouse antibody to detect endogenous expression. Please let me know.

      Read More

      Abcam community

      Verified customer

      Asked on May 21 2012

      Answer

      Thank you for contacting us.

      We have a number of antibodies to CD36 that are guaranteed to detect endogenous expression. For example, we have the mouse monoclonal ab17044 which reacts with human and rat for use in blocking, ICC/IF, WB, IHC-P, IHC-Fr, and Flow Cytometry. We also have the rat monoclonal ab80080 which reacts with mouse and human for use in IHC-P, WB, IP, functional studies, Flow Cytometry, and ICC/IF.

      If you could please let me know what species and application you are working in, I would be happy to make more specific recommendations.

      Read More

      Abcam Scientific Support

      Answered on May 21 2012

      Question

      Please recommend an antibody that will inhibit function of murine CD36.

      Read More

      Abcam community

      Verified customer

      Asked on Dec 15 2011

      Answer

      Thank you for contacting us. The following article describes development of the ab80080 clone, MF3, and how it was used in a neutralization assay. As I mentioned in our conversation, you will want to decide if the neutralization assay has any relevance to what you want to do. Helming L et al. The scavenger receptor CD36 plays a role in cytokine-induced macrophage fusion. J Cell Sci 122:453-9 (2009). PubMed: 19155290 Here is a URL link to the article: http://jcs.biologists.org/content/122/4/453.full I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Dec 15 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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