Key features and details
- Rat monoclonal [MF3] to CD36 - Low endotoxin, Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG2a
Product nameAnti-CD36 antibody [MF3] - Low endotoxin, Azide free
See all CD36 primary antibodies
DescriptionRat monoclonal [MF3] to CD36 - Low endotoxin, Azide free
SpecificityAntibodies produced by clone MF3 have been shown to inhibit IL4 induced thioglycollate-elicited peritoneal macrophage fusion and significantly block IL4/GM-CSF-induced bone-marrorw derived macrophage fusion.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse, Human
IL4 treated murine thioglycollate-elicited peritoneal macrophages.
- Mouse peripheral blood platelets.
General notesEndotoxin Level <0.01EU/ug.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Concentration information loading...
PurityProtein G purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab80080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
FunctionMultifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity) (PubMed:18353783, PubMed:21610069). In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity) (PubMed:18753675). Involved in oral fat perception and preferences (PubMed:22240721, PubMed:25822988). Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellulare calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity) (PubMed:20037584). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity) (PubMed:16880211).
(Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling.
Involvement in diseasePlatelet glycoprotein IV deficiency
Coronary heart disease 7
Sequence similaritiesBelongs to the CD36 family.
modificationsN-glycosylated and O-glycosylated with a ratio of 2:1.
Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome (PubMed:21610069, PubMed:18353783). Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids (PubMed:18353783).
Cellular localizationCell membrane. Membrane raft. Golgi apparatus. Apical cell membrane. Upon ligand-binding, internalized through dynamin-dependent endocytosis.
- Information by UniProt
- Adipocyte membrane protein antibody
- BDPLT10 antibody
- CD36 antibody
Figure A. RPE conjugated Rat anti Mouse CD11b and Pacific Blue conjugated Rat IgG2a isotype control. Figure B. RPE conjugated Rat anti Mouse CD11b and Pacific Blue conjugated Rat anti Mouse CD36. All experiments performed on mouse peritoneal macrophages gated on live, single cells in the presence of 10% mouse serum. Data acquired on the ZE5™ Cell Analyzer
ab80080 has been referenced in 13 publications.
- Xie Z et al. In vivo assessment of inflammation in carotid atherosclerosis by noninvasive photoacoustic imaging. Theranostics 10:4694-4704 (2020). PubMed: 32292523
- Cao X et al. Y-box binding protein 1 regulates ox-LDL mediated inflammatory responses and lipid uptake in macrophages. Free Radic Biol Med 141:10-20 (2019). PubMed: 31153975
- Li X et al. Advanced Oxidation Protein Products Promote Lipotoxicity and Tubulointerstitial Fibrosis via CD36/ß-Catenin Pathway in Diabetic Nephropathy. Antioxid Redox Signal 31:521-538 (2019). PubMed: 31084358
- Tan T et al. Bioinspired lipoproteins-mediated photothermia remodels tumor stroma to improve cancer cell accessibility of second nanoparticles. Nat Commun 10:3322 (2019). PubMed: 31346166
- Sugisawa R et al. Independent modes of disease repair by AIM protein distinguished in AIM-felinized mice. Sci Rep 8:13157 (2018). PubMed: 30177770