Overview

  • Product name
    Anti-CD38 antibody [90] - BSA and Azide free
    See all CD38 primary antibodies
  • Description
    Rat monoclonal [90] to CD38 - BSA and Azide free
  • Host species
    Rat
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse CD38. Mouse pre-B cells.

  • Positive control
    • Flow Cyt: C57/BL6 splenocytes.
  • General notes

    ab239595 is a PBS-only buffer format of ab61400. Please refer to ab61400 for recommended dilutions, protocols, and image data.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    In mouse CD38 is expressed by follicular B cells, is down regulated on germinal centre B cells, and not expressed by mature plasma cells. This pattern of expression contrasts strikingly with that seen in the human.

Applications

Our Abpromise guarantee covers the use of ab239595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Target

  • Function
    Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.
  • Tissue specificity
    Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.
  • Sequence similarities
    Belongs to the ADP-ribosyl cyclase family.
  • Developmental stage
    Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Form
    There are 2 isoforms produced by alternative splicing.
  • Alternative names
    • Acute lymphoblastic leukemia cells antigen CD38 antibody
    • ADP ribosyl cyclase 1 antibody
    • ADP ribosyl cyclase antibody
    • ADP ribosyl cyclase/cyclic ADP-ribose hydrolase antibody
    • ADP-ribosyl cyclase 1 antibody
    • ADPRC 1 antibody
    • ADPRC1 antibody
    • cADPr hydrolase 1 antibody
    • CD 38 antibody
    • CD38 antibody
    • CD38 antigen (p45) antibody
    • CD38 antigen antibody
    • CD38 molecule antibody
    • Cd38-rs1 antibody
    • CD38_HUMAN antibody
    • CD38H antibody
    • Cyclic ADP ribose hydrolase antibody
    • Cyclic ADP ribose hydrolase 1 antibody
    • Cyclic ADP-ribose hydrolase 1 antibody
    • EC 3.2.2.5 antibody
    • Ecto nicotinamide adenine dinucleotide glycohydrolase antibody
    • I-19 antibody
    • I19 (mouse) antibody
    • Lymphocyte differentiation antigen CD38 antibody
    • NAD(+) nucleosidase antibody
    • NIM-R5 antigen antibody
    • NIMR5 antigen (mouse) antibody
    • OTTHUMP00000158633 antibody
    • OTTHUMP00000217743 antibody
    • p45 antibody
    • T10 antibody
    see all

Images

  • C57BL/6 mouse splenocytes stained with ab61400 (right) or Rat IgG2aΚ (monoclonal) (left). C57BL/6 Mouse splenocytes were incubated for 30 min on ice in 1x PBS containing 10 µg/ml anti CD16/32 antibody and 10% normal goat serum to block FC receptors and non-specific protein-protein interactions followed by the antibody (ab61400) or Rat IgG2a Isotype (1x106 in 100µl at 1 µg/ml) (left) for 30 min on ice.

    The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150165) was used at 1/2000 dilution for 30 min at 4°. The cells were simultaneously stained with CD19 APC conjugated antibody.

    Acquisition of >50,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab61400).

References

This product has been referenced in:
  • Oliver AM  et al. Mouse CD38 is down-regulated on germinal center B cells and mature plasma cells. J Immunol 158:1108-15 (1997). Read more (PubMed: 9013949) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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