Anti-CD38 antibody (ab244390)
Key features and details
- Rabbit polyclonal to CD38
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD38 antibody
See all CD38 primary antibodies -
Description
Rabbit polyclonal to CD38 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey -
Immunogen
Recombinant fragment corresponding to Human CD38 aa 150 to the C-terminus.
Database link: P28907 -
Positive control
- IHC-P: Human tonsil, rectum and tonsil tissue. WB: Daudi cell lysate. CD38 overexpression HEK-293T cell lysate. ICC/IF: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. -
Tissue specificity
Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. -
Sequence similarities
Belongs to the ADP-ribosyl cyclase family. -
Developmental stage
Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 952 Human
- Omim: 107270 Human
- SwissProt: Q5VAN0 Cynomolgus monkey
- SwissProt: P28907 Human
- Unigene: 479214 Human
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Form
There are 2 isoforms produced by alternative splicing. -
Alternative names
- Acute lymphoblastic leukemia cells antigen CD38 antibody
- ADP ribosyl cyclase 1 antibody
- ADP ribosyl cyclase antibody
see all
Images
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Paraffin-embedded human rectum tissue stained for CD38 using ab244390 at 1/1000 dilution in immunohistochemical analysis.
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Paraffin-embedded human prostate tissue stained for CD38 using ab244390 at 1/1000 dilution in immunohistochemical analysis.
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PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells stained for CD38 (green) using ab244390 at 4 µg/ml in ICC/IF.
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Paraffin-embedded human tonsil tissue stained for CD38 using ab244390 at 1/1000 dilution in immunohistochemical analysis.
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Paraffin-embedded human kidney tissue stained for CD38 using ab244390 at 1/1000 dilution in immunohistochemical analysis.
Low expression as expected.
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Anti-CD38 antibody (ab244390) at 0.4 µg/ml + Daudi (Human Burkitt's lymphoma cell line) cell lysate
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab244390 has not yet been referenced specifically in any publications.