Recombinant Anti-CD38 antibody [EPR21079] (ab216343)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21079] to CD38
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-CD38 antibody [EPR21079]
See all CD38 primary antibodies -
Description
Rabbit monoclonal [EPR21079] to CD38 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: P388D1 whole cell lysate; Mouse spleen, lymph node, colon and thymus lysates. IHC-P: Mouse spleen, liver and kidney tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21079 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. -
Tissue specificity
Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. -
Sequence similarities
Belongs to the ADP-ribosyl cyclase family. -
Developmental stage
Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12494 Mouse
- SwissProt: P56528 Mouse
- Unigene: 249873 Mouse
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Form
There are 2 isoforms produced by alternative splicing. -
Alternative names
- Acute lymphoblastic leukemia cells antigen CD38 antibody
- ADP ribosyl cyclase 1 antibody
- ADP ribosyl cyclase antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded (A) Spleen tissue from wild-type C57BL/6JGpt mice (B) Spleen tissue from CD38 knockout mice staining with ab216343 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Spleen tissue from wild-type C57BL/6JGpt mice and no staining on (B) Spleen tissue from CD38 knockout mice. The section was incubated with ab216343 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Cd38-KO homozygous mice (Strain ID: T011481). -
Immunohistochemical analysis of paraffin-embedded (A) Kidney tissue from wild-type C57BL/6JGpt mice (B) Kidney tissue from CD38 knockout mice staining with ab216343 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Kidney tissue from wild-type C57BL/6JGpt mice and no staining on (B) Kidney tissue from CD38 knockout mice. The section was incubated with ab216343 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Cd38-KO homozygous mice (Strain ID: T011481).
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Immunohistochemical analysis of paraffin-embedded (A) Liver tissue from wild-type C57BL/6JGpt mice (B) Liver tissue from CD38 knockout mice staining with ab216343 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Liver tissue from wild-type C57BL/6JGpt mice and no staining on (B) Liver tissue from CD38 knockout mice. The section was incubated with ab216343 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND™ RX instrument
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Cd38-KO homozygous mice (Strain ID: T011481). -
All lanes : Anti-CD38 antibody [EPR21079] (ab216343) at 1/1000 dilution
Lane 1 : Wild-type mouse kidney tissue lysate (male)
Lane 2 : CD38 knockout mouse kidney tissue lysate (male)
Lane 3 : Wild-type mouse liver tissue lysate (male)
Lane 4 : CD38 knockout mouse liver tissue lysate (male)
Lane 5 : Wild-type mouse spleen tissue lysate (male)
Lane 6 : CD38 knockout spleen tissue lysate (male)
Lane 7 : Wild-type mouse kidney tissue lysate (female)
Lane 8 : CD38 knockout mouse kidney tissue lysate (female)
Lane 9 : Wild-type mouse liver tissue lysate (female)
Lane 10 : CD38 knockout mouse liver tissue lysate (female)
Lane 11 : Wild-type mouse spleen tissue lysate (female)
Lane 12 : CD38 knockout mouse spleen tissue lysate (female)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 38 seconds
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and CD38-KO homozygous mice (Strain ID: T011481). -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling CD38 with ab216343 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in endothelial cells of mouse kidney (PMID: 25078456). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD38 with ab216343 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in the sinusoids of mouse liver (PMID: 17200192). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD38 with ab216343 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse spleen (PMID: 10360965). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-CD38 antibody [EPR21079] (ab216343) at 1/1000 dilution
Lane 1 : Mouse spleen lysate
Lane 2 : P388D1 (mouse lymphoma monocyte; macrophage cell line) whole cell lysate
Lane 3 : Mouse lymph node lysate
Lane 4 : Mouse colon lysate
Lane 5 : Mouse thymus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 34 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument. The observed molecular mass is consistent with the literature (PMID: 7774637).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab216343 has been referenced in 4 publications.
- de Zélicourt A et al. CD38-NADase is a new major contributor to Duchenne muscular dystrophic phenotype. EMBO Mol Med 14:e12860 (2022). PubMed: 35298089
- Kim HN et al. A decrease in NAD+ contributes to the loss of osteoprogenitors and bone mass with aging. NPJ Aging Mech Dis 7:8 (2021). PubMed: 33795658
- Gan L et al. CD38 deficiency alleviates Ang II-induced vascular remodeling by inhibiting small extracellular vesicle-mediated vascular smooth muscle cell senescence in mice. Signal Transduct Target Ther 6:223 (2021). PubMed: 34112762
- An Z et al. EGFR Cooperates with EGFRvIII to Recruit Macrophages in Glioblastoma. Cancer Res 78:6785-6794 (2018). PubMed: 30401716