Application
Western blot
Sample
Human Cell lysate - whole cell (H1299 cells)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
25 µg
Specification
H1299 cells
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
21 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Licor Odyssey Blocking Buffer
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: IRDye 680 LT
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Detection
Detection method
Licor Odyssey Clx
Negative control
CD38 KO mouse liver
Exposure
12 minute(s) and 0 second(s)
Positive control
WT mouse brain, heart, liver
Additional data
Additional Notes
Antibody reacts very well with mouse tissue but does not appear to have any cross-reactivity with human cells
Green: CD38 (ab216343)
Red: GAPDH (ab8245)
Lane 1: WT mouse brain
Lane 2: WT mouse heart
Lane 3: WT mouse liver
Lane 4: CD38 KO mouse liver
Lane 5: H1299 cell lysate
PVDF membrane RIPA lysis buffer
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested for cross reactivity with human and we were unsure of what the results would be. The antibody may not be suitable for reactivity with this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Sep 21 2018