Recombinant
RabMAb

Recombinant Anti-CD38 antibody [EPR22691-219] (ab235590)

Overview

  • Product name

    Anti-CD38 antibody [EPR22691-219]
    See all CD38 primary antibodies
  • Description

    Rabbit monoclonal [EPR22691-219] to CD38
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P,IP or WB
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse CD38 aa 1-300. The exact sequence is proprietary.
    Database link: P56528

  • Positive control

    • ICC/IF: Mouse splenocytes. Flow cyt: Mouse splenocytes.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab235590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.
ICC/IF 1/50.
  • Application notes
    Is unsuitable for IHC-P,IP or WB.
  • Target

    • Function

      Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.
    • Tissue specificity

      Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.
    • Sequence similarities

      Belongs to the ADP-ribosyl cyclase family.
    • Developmental stage

      Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Form

      There are 2 isoforms produced by alternative splicing.
    • Alternative names

      • Acute lymphoblastic leukemia cells antigen CD38 antibody
      • ADP ribosyl cyclase 1 antibody
      • ADP ribosyl cyclase antibody
      • ADP ribosyl cyclase/cyclic ADP-ribose hydrolase antibody
      • ADP-ribosyl cyclase 1 antibody
      • ADPRC 1 antibody
      • ADPRC1 antibody
      • cADPr hydrolase 1 antibody
      • CD 38 antibody
      • CD38 antibody
      • CD38 antigen (p45) antibody
      • CD38 antigen antibody
      • CD38 molecule antibody
      • Cd38-rs1 antibody
      • CD38_HUMAN antibody
      • CD38H antibody
      • Cyclic ADP ribose hydrolase antibody
      • Cyclic ADP ribose hydrolase 1 antibody
      • Cyclic ADP-ribose hydrolase 1 antibody
      • EC 3.2.2.5 antibody
      • Ecto nicotinamide adenine dinucleotide glycohydrolase antibody
      • I-19 antibody
      • I19 (mouse) antibody
      • Lymphocyte differentiation antigen CD38 antibody
      • NAD(+) nucleosidase antibody
      • NIM-R5 antigen antibody
      • NIMR5 antigen (mouse) antibody
      • OTTHUMP00000158633 antibody
      • OTTHUMP00000217743 antibody
      • p45 antibody
      • T10 antibody
      see all

    Images

    • Flow cytometric analysis of mouse splenocytes labeling CD38 with ab235590 at 1/500 dilution (Right) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left).

      Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

      Cells were stained with rabbit IgG (Left) or ab235590 (Right). Then stained with anti-CD45R conjugated to Alexa Fluor® 647.

      Gated on viable cells.

    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse splenocytes labeling CD38 with ab235590 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in subsets of mouse splenocytes. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS nstead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    References

    ab235590 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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