Anti-CD38 antibody [EPR4106] (ab108403)
- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameAnti-CD38 antibody [EPR4106]
See all CD38 primary antibodies -
DescriptionRabbit monoclonal [EPR4106] to CD38
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Host speciesRabbit
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SpecificityNot suitable for mouse and rat samples because antibody shows a non-specific cytoplasmic staining in almost all tested mouse and rat tissues.
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Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human CD38 (C terminal). The exact sequence is proprietary.
(Peptide available asab194748) -
Positive control
- WB: HeLa, THP-1, Raji and HuT-78 cell lysates, Human plasma and fetal thymus tissue lysates. IHC-P: Human endometrial adenocarcinoma, tonsil, lymph node, skeletal muscle and normal colon tissues.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
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Dissociation constant (KD)KD = 1.40 x 10 -11 M Learn more about KD
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR4106
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab108403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa). For unpurified use at 1/1000 - 1/10000. |
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| IHC-P | 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
Target
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FunctionSynthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.
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Tissue specificityExpressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.
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Sequence similaritiesBelongs to the ADP-ribosyl cyclase family.
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Developmental stagePreferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E.
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Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 952 Human
- Omim: 107270 Human
- SwissProt: P28907 Human
- Unigene: 479214 Human
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FormThere are 2 isoforms produced by alternative splicing.
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Alternative names
- Acute lymphoblastic leukemia cells antigen CD38 antibody
- ADP ribosyl cyclase 1 antibody
- ADP ribosyl cyclase antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)](https://www.abcam.com/ps/products/108/ab108403/Images/ab108403-9-ab108403IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)](https://www.abcam.com/ps/products/108/ab108403/Images/CD38-Primary-antibodies-ab108403-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.
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![Western blot - Anti-CD38 antibody [EPR4106] (ab108403)](https://www.abcam.com/ps/products/108/ab108403/Images/ab108403-8-ab108403WB1.jpg)
Western blot - Anti-CD38 antibody [EPR4106] (ab108403)All lanes : Anti-CD38 antibody [EPR4106] (ab108403) at 1/2000 dilution (purified)
Lanes 1 & 3 : HuT-78 cell lysate
Lane 2 : Human fetal thymus tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 45 kDa (why is the actual band size different from the predicted?)Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-CD38 antibody [EPR4106] (ab108403)](https://www.abcam.com/ps/products/108/ab108403/Images/CD38-Primary-antibodies-ab108403-1.gif)
Western blot - Anti-CD38 antibody [EPR4106] (ab108403)All lanes : Anti-CD38 antibody [EPR4106] (ab108403) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : Human plasma cell lysate
Lane 3 : THP-1 cell lysate
Lane 4 : HuT-78 cell lysate
Lane 5 : Human fetal thymus cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 34 kDa
Observed band size: 45 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)](https://www.abcam.com/ps/products/108/ab108403/Images/ab108403-10-ab108403IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)](https://www.abcam.com/ps/products/108/ab108403/Images/CD38-Primary-antibodies-ab108403-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial adenocarcinoma tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)](https://www.abcam.com/ps/products/108/ab108403/Images/CD38-Primary-antibodies-ab108403-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling CD38 with unpurified ab108403.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)](https://www.abcam.com/ps/products/108/ab108403/Images/CD38-Primary-antibodies-ab108403-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human colon tissue labelling CD38 with unpurified ab108403.
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![Other - Anti-CD38 antibody [EPR4106] (ab108403)](https://www.abcam.com/ps/products/108/ab108403/Images/CD38-Primary-antibodies-ab108403-7.jpg)
Other - Anti-CD38 antibody [EPR4106] (ab108403)
References
This product has been referenced in:
- Esch MB et al. Multi-cellular 3D human primary liver cell culture elevates metabolic activity under fluidic flow. Lab Chip 15:2269-77 (2015). Read more (PubMed: 25857666) »
- Ceroni F et al. A deletion involving CD38 and BST1 results in a fusion transcript in a patient with autism and asthma. Autism Res 7:254-63 (2014). Read more (PubMed: 24634087) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"