Recombinant
RabMAb

Recombinant Anti-CD38 antibody [EPR4106] (ab108403)

Submit a review Q&A (1)References (7)

Overview

  • Product name

    Anti-CD38 antibody [EPR4106]
    See all CD38 primary antibodies

  • Description

    Rabbit monoclonal [EPR4106] to CD38

  • Host species

    Rabbit

  • Specificity

    Not suitable for mouse and rat samples because antibody shows a non-specific cytoplasmic staining in almost all tested mouse and rat tissues.

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human CD38 (C terminal). The exact sequence is proprietary.
    (Peptide available as ab194748)

  • Positive control

    • WB: HeLa, THP-1, Raji and HuT-78 cell lysates, Human plasma and fetal thymus tissue lysates. IHC-P: Human endometrial adenocarcinoma, tonsil, lymph node, skeletal muscle and normal colon tissues. Flow Cyt: Raji cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab108403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).

For unpurified use at 1/1000 - 1/10000.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.

    • Tissue specificity

      Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.

    • Sequence similarities

      Belongs to the ADP-ribosyl cyclase family.

    • Developmental stage

      Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Form

        There are 2 isoforms produced by alternative splicing.

      • Alternative names

        • Acute lymphoblastic leukemia cells antigen CD38 antibody
        • ADP ribosyl cyclase 1 antibody
        • ADP ribosyl cyclase antibody
        • ADP ribosyl cyclase/cyclic ADP-ribose hydrolase antibody
        • ADP-ribosyl cyclase 1 antibody
        • ADPRC 1 antibody
        • ADPRC1 antibody
        • cADPr hydrolase 1 antibody
        • CD 38 antibody
        • CD38 antibody
        • CD38 antigen (p45) antibody
        • CD38 antigen antibody
        • CD38 molecule antibody
        • Cd38-rs1 antibody
        • CD38_HUMAN antibody
        • CD38H antibody
        • Cyclic ADP ribose hydrolase antibody
        • Cyclic ADP ribose hydrolase 1 antibody
        • Cyclic ADP-ribose hydrolase 1 antibody
        • EC 3.2.2.5 antibody
        • Ecto nicotinamide adenine dinucleotide glycohydrolase antibody
        • I-19 antibody
        • I19 (mouse) antibody
        • Lymphocyte differentiation antigen CD38 antibody
        • NAD(+) nucleosidase antibody
        • NIM-R5 antigen antibody
        • NIMR5 antigen (mouse) antibody
        • OTTHUMP00000158633 antibody
        • OTTHUMP00000217743 antibody
        • p45 antibody
        • T10 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      • Western blot - Anti-CD38 antibody [EPR4106] (ab108403)
        Western blot - Anti-CD38 antibody [EPR4106] (ab108403)
        All lanes : Anti-CD38 antibody [EPR4106] (ab108403) at 1/2000 dilution (purified)

        Lanes 1 & 3 : HuT-78 cell lysate
        Lane 2 : Human fetal thymus tissue lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

        Predicted band size: 34 kDa
        Observed band size: 45 kDa
        why is the actual band size different from the predicted?



        Blocking and dilution buffer: 5% NFDM/TBST.

      • Flow Cytometry - Anti-CD38 antibody [EPR4106] (ab108403)
        Flow Cytometry - Anti-CD38 antibody [EPR4106] (ab108403)

        Flow cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labelling CD38 with ab108403 at 1/900 dilution (1.03 µg/ml) (Red). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Western blot - Anti-CD38 antibody [EPR4106] (ab108403)
        Western blot - Anti-CD38 antibody [EPR4106] (ab108403)
        All lanes : Anti-CD38 antibody [EPR4106] (ab108403) at 1/1000 dilution (unpurified)

        Lane 1 : HeLa cell lysate
        Lane 2 : Human plasma cell lysate
        Lane 3 : THP-1 cell lysate
        Lane 4 : HuT-78 cell lysate
        Lane 5 : Human fetal thymus cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 34 kDa
        Observed band size: 45 kDa why is the actual band size different from the predicted?

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial adenocarcinoma tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling CD38 with unpurified ab108403.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human colon tissue labelling CD38 with unpurified ab108403.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Other - Anti-CD38 antibody [EPR4106] (ab108403)
        Other - Anti-CD38 antibody [EPR4106] (ab108403)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      This product has been referenced in:

      • Huang YH  et al. Decoration of Anti-CD38 on Nanoparticles Carrying a STAT3 Inhibitor Can Improve the Therapeutic Efficacy Against Myeloma. Cancers (Basel) 11:N/A (2019). Read more (PubMed: 30791634) »
      • Ning L  et al. Quantitative Proteomic Analysis Reveals the Deregulation of Nicotinamide Adenine Dinucleotide Metabolism and CD38 in Inflammatory Bowel Disease. Biomed Res Int 2019:3950628 (2019). Read more (PubMed: 31179321) »
      See all 7 Publications for this product

      Publishing research using ab108403? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Good morning,

      I wish to have specific information for the monoclonal antibodies of human CD38 available on your web site. More specifically, I would like to know the nature of the immunogen used to raise those monoclonal antibodies (e.g. amino acid sequence, or CD38 fragment). In other words, I am trying to identify the region of the CD38 protein where these antibodies bind.

      The following catalogue # are the monoclonal antibodies for CD38 available from your company:

      ab2577
      ab93941
      ab108403
      ab49644
      ab1260
      ab34283
      ab75231
      ab91390
      ab9504

      I truly hope you will be able to assist me or orient me toward someone else who could help answering my questions.

      Read More
      Answer

      Thank you for your enquiry.

      Having contacted the originators of these antibodies, we can let you know the following about the immunogens:

      ab2577: Tissue / cell preparation (Human thymocytes in foetus). According to our information the epitope mapping was not done. Only its location to the extracellular portion of the protein is known.

      ab93941: Purified recombinant fragment of Human CD38 expressed in E. coli.The peptide maps between amino acids 171 and 292.

      ab108403: A synthetic peptide corresponding to residues on the C terminus of Human CD38. The peptide maps between amino acids 275 and 300, on the C-terminus of human CD38 (UniProt: P28907).

      ab49644: Recombinant fusion protein corresponding to the extracellular region of human CD38 molecule. Unfortunately, futher details of this immunogen sequence are not available.

      ab1260: I am sorry but we do know the immunogen sequence or the range of amino acids or the region of the peptide from which it is derived. The producer of the hybridoma did not give us this kind of information.

      ab34283: The details of the immunogen for this antibody are also not available.

      ab75231: The antibody, CD 38 was raised to a prokaryotic recombinant protein corresponding to the extracellular region of the CD38 molecule. This region corresponds to amino acids 45-300. Unfortunately, the antibody has not been epitope mapped in order to determine the exact amino acid sequence to which it binds.

      ab91390: This monoclonal was development using as immunogen whole cell of human acute lymphoblastic B leukaemia. The antibody has also not been epitope mapped.

      ab9504: Nawalma cell line. The antibody has also not been epitope mapped.

      We aim to provide as much information as possible to customers, so I am sorry that this has not entirely been possible on this occasion. I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information, or if we could be of any help in choosing the most suitable antibody for your experimental layout.

      Read More

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