Recombinant Anti-CD38 antibody [EPR4106] (ab108403)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4106] to CD38
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-CD38 antibody [EPR4106]
See all CD38 primary antibodies
Description

Rabbit monoclonal [EPR4106] to CD38
Host species

Rabbit
Specificity

Not suitable for mouse and rat samples because antibody shows a non-specific cytoplasmic staining in almost all tested mouse and rat tissues.

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Raji and Ramos cell lysates, Human lymph node lysate. IHC-P: Human endometrial adenocarcinoma, tonsil, lymph node, skeletal muscle and normal colon tissues. Flow Cyt: Raji cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 1.40 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4106
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Recombinant Protein
- Recombinant Human CD38 protein (ab174013)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

Application	Abreviews	Notes
WB		1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa). For unpurified use at 1/1000 - 1/10000.
IHC-P		1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250.
Flow Cyt		1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa). For unpurified use at 1/1000 - 1/10000.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250.
Flow Cyt 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes

Is unsuitable for ICC/IF.

Function

Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.
Tissue specificity

Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.
Sequence similarities

Belongs to the ADP-ribosyl cyclase family.
Developmental stage

Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E.
Cellular localization

Membrane.
Target information above from: UniProt accession P28907 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 952 Human
- Omim: 107270 Human
- SwissProt: P28907 Human
- Unigene: 479214 Human
Form

There are 2 isoforms produced by alternative splicing.
Alternative names
- Acute lymphoblastic leukemia cells antigen CD38 antibody
- ADP ribosyl cyclase 1 antibody
- ADP ribosyl cyclase antibody
- ADP ribosyl cyclase/cyclic ADP-ribose hydrolase antibody
- ADP-ribosyl cyclase 1 antibody
- ADPRC 1 antibody
- ADPRC1 antibody
- cADPr hydrolase 1 antibody
- CD 38 antibody
- CD38 antibody
- CD38 antigen (p45) antibody
- CD38 antigen antibody
- CD38 molecule antibody
- Cd38-rs1 antibody
- CD38_HUMAN antibody
- CD38H antibody
- Cyclic ADP ribose hydrolase antibody
- Cyclic ADP ribose hydrolase 1 antibody
- Cyclic ADP-ribose hydrolase 1 antibody
- EC 3.2.2.5 antibody
- Ecto nicotinamide adenine dinucleotide glycohydrolase antibody
- I-19 antibody
- I19 (mouse) antibody
- Lymphocyte differentiation antigen CD38 antibody
- NAD(+) nucleosidase antibody
- NIM-R5 antigen antibody
- NIMR5 antigen (mouse) antibody
- OTTHUMP00000158633 antibody
- OTTHUMP00000217743 antibody
- p45 antibody
- T10 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-CD38 antibody [EPR4106] (ab108403)

Lane 1 : Anti-CD38 antibody [EPR4106] (ab108403) at 1/200 dilution
Lane 2 : Anti-CD38 antibody [EPR4106] (ab108403) at 1/1000 dilution

Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte), Whole cell lysate
Lane 2 : Ramos (Human Burkitt's lymphoma B lymphocyte), Whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

Predicted band size: 34 kDa

Buffer and concentration: 5% NFDM/TBST

Exposure time, Lane 1: 20 seconds, Lane 2: 30 seconds
Western blot - Anti-CD38 antibody [EPR4106] (ab108403)

Anti-CD38 antibody [EPR4106] (ab108403) at 1/3000 dilution + Human lymph node lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 34 kDa

Exposure time: 80 seconds

Buffer and concentration: 5% NFDM/TBST
Western blot - Anti-CD38 antibody [EPR4106] (ab108403)

All lanes : Anti-CD38 antibody [EPR4106] (ab108403) at 1/2000 dilution (purified)

Lane 1 : HuT-78 cell lysate
Lane 2 : Human fetal thymus tissue lysate
Lane 3 : Raji cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 34 kDa
Observed band size: 45 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-CD38 antibody [EPR4106] (ab108403)

Flow cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labelling CD38 with ab108403 at 1/900 dilution (1.03 µg/ml) (Red). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial adenocarcinoma tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling CD38 with unpurified ab108403.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human colon tissue labelling CD38 with unpurified ab108403.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
OI-RD Scanning - Anti-CD38 antibody [EPR4106] (ab108403)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-CD38 antibody [EPR4106] (ab108403)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

Publishing research using ab108403? Please let us know so that we can cite the reference in this datasheet.

ab108403 has been referenced in 11 publications.

Wang YX et al. Curative effect of methotrexate combined with teniposide in the treatment of primary central nervous system lymphoma. Oncol Lett 19:2097-2106 (2020). PubMed: 32194707
Damond N et al. A Map of Human Type 1 Diabetes Progression by Imaging Mass Cytometry. Cell Metab 29:755-768.e5 (2019). PubMed: 30713109
Huang YH et al. Decoration of Anti-CD38 on Nanoparticles Carrying a STAT3 Inhibitor Can Improve the Therapeutic Efficacy Against Myeloma. Cancers (Basel) 11:N/A (2019). PubMed: 30791634
Ning L et al. Quantitative Proteomic Analysis Reveals the Deregulation of Nicotinamide Adenine Dinucleotide Metabolism and CD38 in Inflammatory Bowel Disease. Biomed Res Int 2019:3950628 (2019). PubMed: 31179321
Chen PY et al. Functional Change of Effector Tumor-Infiltrating CCR5+CD38+HLA-DR+CD8+ T Cells in Glioma Microenvironment. Front Immunol 10:2395 (2019). PubMed: 31649684

View all Publications for this product

Customer reviews and Q&As

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Question

Good morning,

I wish to have specific information for the monoclonal antibodies of human CD38 available on your web site. More specifically, I would like to know the nature of the immunogen used to raise those monoclonal antibodies (e.g. amino acid sequence, or CD38 fragment). In other words, I am trying to identify the region of the CD38 protein where these antibodies bind.

The following catalogue # are the monoclonal antibodies for CD38 available from your company:

ab2577
ab93941
ab108403
ab49644
ab1260
ab34283
ab75231
ab91390
ab9504

I truly hope you will be able to assist me or orient me toward someone else who could help answering my questions.

Abcam community

Verified customer

Asked on Mar 12 2012

Answer

Thank you for your enquiry.

Having contacted the originators of these antibodies, we can let you know the following about the immunogens:

ab2577: Tissue / cell preparation (Human thymocytes in foetus). According to our information the epitope mapping was not done. Only its location to the extracellular portion of the protein is known.

ab93941: Purified recombinant fragment of Human CD38 expressed in E. coli.The peptide maps between amino acids 171 and 292.

ab108403: A synthetic peptide corresponding to residues on the C terminus of Human CD38. The peptide maps between amino acids 275 and 300, on the C-terminus of human CD38 (UniProt: P28907).

ab49644: Recombinant fusion protein corresponding to the extracellular region of human CD38 molecule. Unfortunately, futher details of this immunogen sequence are not available.

ab1260: I am sorry but we do know the immunogen sequence or the range of amino acids or the region of the peptide from which it is derived. The producer of the hybridoma did not give us this kind of information.

ab34283: The details of the immunogen for this antibody are also not available.

ab75231: The antibody, CD 38 was raised to a prokaryotic recombinant protein corresponding to the extracellular region of the CD38 molecule. This region corresponds to amino acids 45-300. Unfortunately, the antibody has not been epitope mapped in order to determine the exact amino acid sequence to which it binds.

ab91390: This monoclonal was development using as immunogen whole cell of human acute lymphoblastic B leukaemia. The antibody has also not been epitope mapped.

ab9504: Nawalma cell line. The antibody has also not been epitope mapped.

We aim to provide as much information as possible to customers, so I am sorry that this has not entirely been possible on this occasion. I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information, or if we could be of any help in choosing the most suitable antibody for your experimental layout.

Abcam Scientific Support

Answered on Mar 12 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Overview

Product name

Description

Host species

Specificity

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Developmental stage

Cellular localization

Database links

Form

Alternative names

Images

Protocols

Datasheets and documents

References (11)

Customer reviews and Q&As

Dissociation constant (K_D)