Recombinant
RabMAb

Recombinant Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)

Overview

  • Product name
    Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free
    See all CD38 primary antibodies
  • Description
    Rabbit monoclonal [EPR4106] to CD38 - Low endotoxin, Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    ab176886 is a PBS-only buffer formulated version of ab108403, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab108403 for information on protocols, dilutions, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab176886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).

Target

  • Function
    Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.
  • Tissue specificity
    Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.
  • Sequence similarities
    Belongs to the ADP-ribosyl cyclase family.
  • Developmental stage
    Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Form
    There are 2 isoforms produced by alternative splicing.
  • Alternative names
    • Acute lymphoblastic leukemia cells antigen CD38 antibody
    • ADP ribosyl cyclase 1 antibody
    • ADP ribosyl cyclase antibody
    • ADP ribosyl cyclase/cyclic ADP-ribose hydrolase antibody
    • ADP-ribosyl cyclase 1 antibody
    • ADPRC 1 antibody
    • ADPRC1 antibody
    • cADPr hydrolase 1 antibody
    • CD 38 antibody
    • CD38 antibody
    • CD38 antigen (p45) antibody
    • CD38 antigen antibody
    • CD38 molecule antibody
    • Cd38-rs1 antibody
    • CD38_HUMAN antibody
    • CD38H antibody
    • Cyclic ADP ribose hydrolase antibody
    • Cyclic ADP ribose hydrolase 1 antibody
    • Cyclic ADP-ribose hydrolase 1 antibody
    • EC 3.2.2.5 antibody
    • Ecto nicotinamide adenine dinucleotide glycohydrolase antibody
    • I-19 antibody
    • I19 (mouse) antibody
    • Lymphocyte differentiation antigen CD38 antibody
    • NAD(+) nucleosidase antibody
    • NIM-R5 antigen antibody
    • NIMR5 antigen (mouse) antibody
    • OTTHUMP00000158633 antibody
    • OTTHUMP00000217743 antibody
    • p45 antibody
    • T10 antibody
    see all

Images

  • This IHC data was generated using the same anti-CD38 antibody clone, EPR4106, in a different buffer formulation (cat# ab108403).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • This IHC data was generated using the same anti-CD38 antibody clone, EPR4106, in a different buffer formulation (cat# ab108403).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial adenocarcinoma tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108403).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108403).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling CD38 with unpurified ab108403.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108403).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human colon tissue labelling CD38 with unpurified ab108403.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108403).

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108403).

References

This product has been referenced in:
See 1 Publication for this product

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