Recombinant Anti-CD38 antibody [HB-7] (ab235118)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CD38 antibody [HB-7]
See all CD38 primary antibodies -
Description
Mouse monoclonal [HB-7] to CD38 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- ICC/IF: Raji cells. Human PBMC. FC: Raji cells. Human PBMC.
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General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HB-7 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab235118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | 1/100. | |
Flow Cyt | 1/500. |
Target
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Function
Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. -
Tissue specificity
Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. -
Sequence similarities
Belongs to the ADP-ribosyl cyclase family. -
Developmental stage
Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 952 Human
- Omim: 107270 Human
- SwissProt: P28907 Human
- Unigene: 479214 Human
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Form
There are 2 isoforms produced by alternative splicing. -
Alternative names
- Acute lymphoblastic leukemia cells antigen CD38 antibody
- ADP ribosyl cyclase 1 antibody
- ADP ribosyl cyclase antibody
see all
Images
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD38 with ab235118 at 1/100 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in Raji cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at a 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (red).The negative controls are as follows:
-ve control 1: ab235118 at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution. -
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMC (human primary peripheral blood mononuclear cell) cells labeling CD38 with ab235118 at 1/100 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in subsets of human PBMC. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at a 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (red).The negative controls are as follows:
-ve control 1: ab235118 at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution. -
Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cell line labeling CD38 with ab235118 at 1/500 dilution (red) compared with a Mouse IgG, monoclonal - Isotype Control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) (right) labeling CD38 with ab235118 at 1/500 dilution compared with Mouse monoclonal IgG Isotype Control (left). Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Datasheets and documents
References
ab235118 has not yet been referenced specifically in any publications.