Recombinant Anti-CD38 antibody [HB-7] (ab235118)
![Flow Cytometry - Anti-CD38 antibody [HB-7] (ab235118)](https://www.abcam.com/ps/products/235/ab235118/Images/ab235118-366395-AntiCD38-antibody-HB7-immunocytochemistry-raji-human.jpg "Flow Cytometry - Anti-CD38 antibody [HB-7] (ab235118)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [HB-7] to CD38
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-CD38 antibody [HB-7]
See all CD38 primary antibodies -
Description
Mouse monoclonal [HB-7] to CD38 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD38. Human BJAB cell line
-
Positive control
- ICC/IF: Raji cells. Human PBMC.Flow Cyt: Raji cells. Human PBMC.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HB-7 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 5.6 µg/ml.
|
|
| Flow Cyt |
Use a concentration of 1.12 µg/ml.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 5.6 µg/ml. |
|
Flow Cyt
Use a concentration of 1.12 µg/ml. |
Target
-
Function
Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. -
Tissue specificity
Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. -
Sequence similarities
Belongs to the ADP-ribosyl cyclase family. -
Developmental stage
Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 952 Human
- Omim: 107270 Human
- SwissProt: P28907 Human
- Unigene: 479214 Human
-
Form
There are 2 isoforms produced by alternative splicing. -
Alternative names
- Acute lymphoblastic leukemia cells antigen CD38 antibody
- ADP ribosyl cyclase 1 antibody
- ADP ribosyl cyclase antibody
see all
Images
-
Flow Cytometry - Anti-CD38 antibody [HB-7] (ab235118)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD38 with ab235118 at 5.6µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in Raji cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at a 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (red).The negative controls are as follows:
-ve control 1: ab235118 at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-CD38 antibody [HB-7] (ab235118)](https://www.abcam.com/ps/products/235/ab235118/Images/ab235118-366394-AntiCD38-antibody-HB7-immunocytochemistry-pbmc-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD38 antibody [HB-7] (ab235118)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMC (human primary peripheral blood mononuclear cell) cells labeling CD38 with ab235118 at 5.6µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in subsets of human PBMC. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at a 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (red).The negative controls are as follows:
-ve control 1: ab235118 at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution. -
![Flow Cytometry - Anti-CD38 antibody [HB-7] (ab235118)](https://www.abcam.com/ps/products/235/ab235118/Images/ab235118-366393-AntiCD38-antibody-HB7-flow-cytometry-raji-human.jpg)
Flow Cytometry - Anti-CD38 antibody [HB-7] (ab235118)Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cell line labeling CD38 with ab235118 at 1.12µg/ml (red) compared with a Mouse IgG, monoclonal - Isotype Control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
-
![Flow Cytometry - Anti-CD38 antibody [HB-7] (ab235118)](https://www.abcam.com/ps/products/235/ab235118/Images/ab235118-366392-AntiCD38-antibody-HB7-flow-cytometry-pbmc-human.jpg)
Flow Cytometry - Anti-CD38 antibody [HB-7] (ab235118)Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) (right) labeling CD38 with ab235118 at 1.12µg/ml compared with Mouse monoclonal IgG Isotype Control (left). Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
-
![Anti-CD38 antibody [HB-7] (ab235118)](https://www.abcam.com/ps/products/235/ab235118/Images/ab235118-5-benefits-of-recombinant-antibodies.png)
Anti-CD38 antibody [HB-7] (ab235118)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab235118 has not yet been referenced specifically in any publications.
