Recombinant Anti-CD38 antibody [HB-7] (ab235118)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [HB-7] to CD38
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CD38 antibody [HB-7]
See all CD38 primary antibodies -
Description
Mouse monoclonal [HB-7] to CD38 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD38. Human BJAB cell line
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Positive control
- ICC/IF: Raji cells. Human PBMC.Flow Cyt: Raji cells. Human PBMC.
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General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HB-7 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab235118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5.6 µg/ml. | |
| Flow Cyt | Use a concentration of 1.12 µg/ml. |
Target
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Function
Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. -
Tissue specificity
Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. -
Sequence similarities
Belongs to the ADP-ribosyl cyclase family. -
Developmental stage
Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 952 Human
- Omim: 107270 Human
- SwissProt: P28907 Human
- Unigene: 479214 Human
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Form
There are 2 isoforms produced by alternative splicing. -
Alternative names
- Acute lymphoblastic leukemia cells antigen CD38 antibody
- ADP ribosyl cyclase 1 antibody
- ADP ribosyl cyclase antibody
see all
Images
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![Flow Cytometry - Anti-CD38 antibody [HB-7] (ab235118)](https://www.abcam.com/ps/products/235/ab235118/Images/ab235118-366395-AntiCD38-antibody-HB7-immunocytochemistry-raji-human.jpg)
Flow Cytometry - Anti-CD38 antibody [HB-7] (ab235118)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD38 with ab235118 at 5.6µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in Raji cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at a 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (red).The negative controls are as follows:
-ve control 1: ab235118 at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-CD38 antibody [HB-7] (ab235118)](https://www.abcam.com/ps/products/235/ab235118/Images/ab235118-366394-AntiCD38-antibody-HB7-immunocytochemistry-pbmc-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD38 antibody [HB-7] (ab235118)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMC (human primary peripheral blood mononuclear cell) cells labeling CD38 with ab235118 at 5.6µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in subsets of human PBMC. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at a 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (red).The negative controls are as follows:
-ve control 1: ab235118 at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution. -
![Flow Cytometry - Anti-CD38 antibody [HB-7] (ab235118)](https://www.abcam.com/ps/products/235/ab235118/Images/ab235118-366393-AntiCD38-antibody-HB7-flow-cytometry-raji-human.jpg)
Flow Cytometry - Anti-CD38 antibody [HB-7] (ab235118)Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cell line labeling CD38 with ab235118 at 1.12µg/ml (red) compared with a Mouse IgG, monoclonal - Isotype Control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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![Flow Cytometry - Anti-CD38 antibody [HB-7] (ab235118)](https://www.abcam.com/ps/products/235/ab235118/Images/ab235118-366392-AntiCD38-antibody-HB7-flow-cytometry-pbmc-human.jpg)
Flow Cytometry - Anti-CD38 antibody [HB-7] (ab235118)Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) (right) labeling CD38 with ab235118 at 1.12µg/ml compared with Mouse monoclonal IgG Isotype Control (left). Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
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![Anti-CD38 antibody [HB-7] (ab235118)](https://www.abcam.com/ps/products/235/ab235118/Images/ab235118-5-benefits-of-recombinant-antibodies.png)
Anti-CD38 antibody [HB-7] (ab235118)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab235118 has not yet been referenced specifically in any publications.
